Cardiac Oxidative Stress and Inflammation Are Similar in SAMP8 and SAMR1 Mice and Unaltered by Curcumin and Ginkgo Biloba Extract Intake
Abstract
Chronic inflammation and oxidative stress increase with advancing age and appear to be involved in the pathogenesis of coronary heart disease, the leading cause of death worldwide. There is a need for animal models that reflect the increases in pro-inflammatory cytokines and oxidative damage observed during aging in humans. We therefore aimed to investigate the suitability of the fast-aging senescence-accelerated mouse-prone 8 (SAMP8) strain and its normally aging control senescence-accelerated mouse-resistant 1 (SAMR1) to study the age-dependent changes in cytokines, oxidative damage and antioxidants in the heart. To this end, 2-months-old male SAMR1 and SAMP8 mice were fed a Western type diet (control groups) for 5 months. Two groups of SAMP8 mice were simultaneously fed identical diets fortified with 0.5 g curcumin or 1.0 g Ginkgo biloba extract EGb 761® per kg diet. Heart tissue homogenates were analysed for protein carbonyls, glutathione, glutathione disulfide, methionine, cysteine and uric acid as well as the cytokines tumor-necrosis factor- α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1. Neither the strain (SAMR1 or SAMP8) nor antioxidant intake (curcumin or EGb 761®) affected the concentrations of the measured parameters. In conclusion, our data do not support the suitability of the SAMP8 and SAMR1 strains as a model to study age-related changes in proinflammatory cytokines and oxidative stress parameters in the heart. Keywords: Aging, antioxidants, curcumin, Ginkgo biloba extract, heart health, inflammation, oxidative stress, senescenceaccelerated mice, cytokines, senescence-accelerated mouse-prone 8 (SAMP8), senescence-accelerated mouse-resistant 1 (SAMR1), protein carbonyls, glutathione, glutathione disulfide, methionine, cysteine, uric acid, tumor-necrosis factor-a, interleukin-1b, interleukin-6, Chronic inflammation, coronary heart disease (CHD), Curcuma longa, terpenelactones, flavonols quercetin, isorhamnetin, kaempferol, phytomedicine, Tissue Homogenates, phosphate-buffered saline, Protein Carbonyls, sulfuric acid, hemocyanin, streptavidin-biotinylated, Enzyme-Linked Immunosorbent Assay (ELISA), Glutathione disulfide, metaphosphoric acid, acetonitrile, isocratic elution, orthophosphoric acid, hydrophilic polypropylene, pathogenesis, atherosclerosis, Thiobarbituric acid reactive substances (TBARS)
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Conflicting studies are present in literature regarding CUR's inability to counteract OS.
“Although an antioxidant action in aging has been widely demonstrated, conflicting studies are present in literature regarding its inability to counteract OS [401], therefore more research is needed to better understand its antioxidant role.”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [401]
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- Authors
- Schiborr, C., P. Eckert, G., Weissenberger, J., E. Muller, W., Schwamm, D., Grune, T., Rimbach, G., & Frank, J.
- Affiliations
- Christian-Albrechts-Universität zu Kiel (DE)
- University of Hohenheim (DE)
- Corresponding author
- Frank, Jan
- Venue
- Current Pharmaceutical Biotechnology
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2010-12-01
- Volume
- 11
- Issue
- 8
- Pages
- 861-867
- ISSN
- 1389-2010 (ISSN-L)
- OpenAlex
- W2126537616
- Cited by
- 14
- References
- 0
- Citations by year
- 2025: 1 · 2022: 1 · 2015: 1 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Pharmaceutical Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- cardiac oxidative stress, cardiac inflammation, SAMP8 mice, SAMR1 mice, senescence-accelerated mice, curcumin, Ginkgo biloba extract EGb 761, inflammatory cytokines, protein carbonyls, glutathione, methionine, cysteine, uric acid, Western diet, coronary artery disease, aging, antioxidants
- MeSH terms
- Ginkgo Extract, Age Factors, Aging, Animals, Antioxidants, Coronary Disease, Curcumin, Female, Heart, Humans, Inflammation, Male, Mice, Inbred C57BL, Mice, Inbred Strains, Plant Extracts, Interleukin-6, Cytokines, Oxidative Stress, Ginkgo biloba, Models, Animal, Protein Carbonylation, Atherosclerosis, Mice, Interleukin-1beta
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