Liposomal Encapsulation Enzymes: From Medical Applications to Kinetic Characteristics
Abstract
Liposomes and nanoliposomes as small vesicles composed of phospholipid bilayer (entrapping one or more hydrophilic or lipophilic components) have recently found several potential applications in medicine and food industry. These vesicles may protect the core materials from moisture, heat and other extreme conditions. They may also provide controlled release of various bioactive agents, including food ingredients at the right place and time. Potential applications of enzyme-loaded liposomes are in the medical or biomedical field, particularly for the enzymereplacement therapy, as well as cheese industry for production of functional foods with improved health beneficial impacts on the consumer. Encapsulation process has a recondite impact on enzymes. In fact, liposome preparation techniques may alter the pH and temperature optima, affinity of the enzyme to substrate (Km), and maximum rate of reaction (Vmax). In addition, in this paper, the impact of process variables on the kinetic characteristics of enzymes encapsulated in liposomes was investigated. Also, the effects of enzyme entrapment in liposomes, prepared by different methods, on the catalytic efficiency of enzyme, as well as its kinetic properties and stability compared to native (free) enzymes has been reviewed.
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The SN% results are in agreement with studies by Hannon et al. (2003), Jahadi and Khosravi-Darani (2017), Kaminaries et al. (2019), and Nuñez et al. (1991).
“Moreover, it resulted is in agreement with the study of other scientists such as Hannon et al. (2003); Jahadi and Khosravi-Darani (2017); Kaminaries et al. (2019); Nuñez et al. (1991).”
From Optimizing the acceleration of Cheddar cheese ripening using response surface methodology by microbial protease without altering its quality features (2021) · cites this paper as Jahadi and Khosravi-Darani (2017)
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- Authors
- Jahadi, M., & Khosravi-Darani, K.
- ORCID
- Jahadi, M.
- Venue
- Mini-Reviews in Medicinal Chemistry
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2017-01-17
- Volume
- 17
- Issue
- 4
- Pages
- 366-370
- ISSN
- 1389-5575 (ISSN-L)
- OpenAlex
- W2489959234
- Cited by
- 38
- References
- 37
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 6 · 2018: 3 · 2017: 2
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mini-Reviews in Medicinal Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Agricultural and Biological Sciences › Food Science › Microencapsulation and Drying Processes
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- liposomes, nanoliposomes, enzyme encapsulation, enzyme kinetics, enzyme replacement therapy, cheese industry, functional foods, controlled release, enzyme stability, encapsulation methods, catalytic efficiency, food enzymes
- MeSH terms
- Enzymes, Hydrogen-Ion Concentration, Kinetics, Liposomes, Medicine, Temperature, Biocatalysis, Hydrophobic and Hydrophilic Interactions
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