miR-25 Mediates Retinal Degeneration Via Inhibiting ITGAV and PEDF in Rat
Abstract
Background Age-related macular degeneration (AMD) is the main cause of irreversible blindness in the elderly. Oxidative stress in retinal pigment epithelium (RPE) is deemed to play a pivotal role in the pathogenesis of AMD. miR-25 functions as an essential modulator in response to oxidative-stress in several cell types, but its function in RPE cells is poorly understood. Objective To explore the roles of miR-25 in RPE cells and in the development of AMD. Methods A rat model of retinal degeneration was induced by sodium iodate (SI). Subretinal injection of antagomiR-25 was performed for the intervention while the scramble as control. Visual responses were recorded with Electroretinogram (ERG). TUNEL assay was performed to detect apoptosis. Phagosome quantification in vivo was performed to evaluate RPE cell function. Oxygen-glucose deprivation treatment was performed to mimic in vitro oxidative stress. Gene expression at mRNA level and protein level were performed by quantitative polymerase chain reaction (qPCR) and Western Blot, respectively. The pigment epithelium derived factor (PEDF) level in the cultured medium was measured by Enzyme-linked immunosorbent assay (ELISA). The interaction between miR-25 and integrin αV (IGTAV) / PEDF 3'UTR was examined by dual luciferase assay. Chromatin immunoprecipitation (ChIP) assay was performed to examine its transcriptional regulation of miR-25. Results Oxidative stress up-regulated miR-25 in RPE cells in very early stage, accompanied b…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
miR-25 contributes to vision loss in a way similar to that observed in AMD.
“As such, miR-25 contributes to vision loss in a way similar to that observed in AMD”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [265]
In a rat model, sodium iodate treatment upregulates miR-25 via STAT3, which downregulates IGTAV and PEDF, impairing phagocytosis.
“In a rat model, sodium iodate treatment leads to upregulation of miR-25 (via STAT3), which subsequently downregulates IGTAV and PEDF, and consequently impairs phagocytosis.”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [265]
Cited by
Sorted by citations
MicroRNAs as Regulators of Phagocytosis
Gierlikowski, W., & Gierlikowska, B. (2022) · Cells · doi:10.3390/cells11091380 · Cited by 25 · see citing works
Oltra, M., Vidal-Gil, L., Maisto, R., Oltra, S. S., Romero, F. J., Sancho-Pelluz, J., & Barcia, J. M. (2019) · Scientific Reports · doi:10.1038/s41598-019-54373-x · Cited by 24 · see citing works
- Authors
- Zhang, J., Wang, J., Zheng, L., Wang, M., Lu, Y., Li, Z., Lian, C., Mao, S., Hou, X., Li, S., Xu, J., Tian, H., Jin, C., Gao, F., Zhang, J., Wang, F., Li, W., Lu, L., & Xu, G.-T.
- Affiliations
- Tongji University (CN)
- Drexel University (US)
- Laboratory of Clinical Visual Science, Department of Regenerative Medicine, and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
- Corresponding author
- Xu, G.-T.
- Venue
- Current Molecular Medicine
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2018-01-05
- Volume
- 17
- Issue
- 5
- Pages
- 359-374
- ISSN
- 1566-5240 (ISSN-L)
- OpenAlex
- W2774694006
- Cited by
- 14
- References
- 0
- Citations by year
- 2024: 1 · 2023: 3 · 2022: 2 · 2021: 6 · 2020: 1 · 2019: 1
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- miR-25, age-related macular degeneration, retinal pigment epithelium, retinal degeneration, oxidative stress, ITGAV, pigment epithelium-derived factor, STAT3 signaling, sodium iodate, microRNA regulation, 3' UTR target genes, visual impairment
- MeSH terms
- Pigment Epithelium-Derived Factor, Animals, Eye Proteins, Macular Degeneration, Nerve Growth Factors, Signal Transduction, Serpins, Rats, Sprague-Dawley, Apoptosis, Oxidative Stress, 3' Untranslated Regions, MicroRNAs, Integrin alpha5, STAT3 Transcription Factor, Rats, Retinal Pigment Epithelium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
