Using hair growth activity, physical stability, and safety tests to study hair tonics containing ethanol extract of licorice (Glycyrrhiza glabra Linn.)
Open accessAbstract
Objective: The purpose of this study was to determine the safety, physical stability, and hair growth activity of ethanol extract of licorice.Methods: In this study, 2.5%, 5%, and 10% licorice extract was formulated into a hair tonic as a tonic is easier to use and is not sticky like a semisoliddosage. The hair growth activity test was conducted by rubbing the hair tonic preparations on rabbit’s backs; subsequently, the hair length, hairthickness, hair weight, and hair density were measured. Quantitative analysis of glycyrrhizic acid from the licorice ethanol extract with a ultravioletspectrophotometer showed a level of about 156.65 mg/g or 15.665%. The physical stability test was performed on samples of the tonic stored at low(4±2°C), room (25±2°C), and high (40±2°C) temperature, and a cycling test was also performed. The safety test was performed using an eye irritationtest that employed the Hen’s egg test–chorioallantoic membrane (HET-CAM) method and a skin irritation test that employed the patch test method.Results: The hair tonics containing 5% and 10% licorice extract had an equivalent activity of hair growth and even better than the positive controlcontaining 2% minoxidil. The physical stability test showed that the licorice extract hair tonic has good physical stability. The results of the safety testshowed no skin irritation, whereas the HET-CAM test showed that the hair tonic containing licorice extract showed mild eye irritation.Conclusions: Licorice ethanol extract hair tonic solutions in concentrations of 2.5%, 5%, and 10% had hair growth activity similar to that of thepositive control (minoxidil). They have a good physical and chemical stability, also safe for topical use, except the 2.5% licorice ethanol extract hairtonic solution which caused mild eye irritation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Hair tonic solutions containing ethanol extract of licorice (G. glabra) showed safe topical use.
“The hair tonic solutions containing this extract showed hair growth activity similar to that of the positive control (minoxidil), good physical and chemical stability, and safe topical use [85].”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [85]
The Conclusion states that the 10% licorice-extract hair tonic was safe for topical use, and the Discussion reports no skin irritation in a test involving 20 volunteers. The claim generalizes that finding to the hair tonic solutions without specifying the tested concentration; the Conclusion also notes that the 2.5% solution irritated the eye. (LLM, conf=0.86)
Experimental — accuracy not yet measuredHair tonic solutions containing ethanol extract of licorice (G. glabra) showed hair growth activity similar to minoxidil.
“The hair tonic solutions containing this extract showed hair growth activity similar to that of the positive control (minoxidil), good physical and chemical stability, and safe topical use [85].”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [85]
Hair tonic solutions containing ethanol extract of licorice (G. glabra) showed good physical and chemical stability.
“The hair tonic solutions containing this extract showed hair growth activity similar to that of the positive control (minoxidil), good physical and chemical stability, and safe topical use [85].”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [85]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 12
- Mitsui T. New Cosmetic Science. Amsterdam: Elsevier Science B.V. Nakayama Shoten Publishers; 1992.
- Wasiaatmadja SM. Penuntun Ilmu Kosmetik Medik. 1 st ed. Jakarta: Universitas Indonesia Press; 1997.
- Purwal L, Gupta S, Pande M. Development and evaluation of herbal formulations for hair growth. EJ Chem 2008;5(1):34-8.
- Lourith N, Kanlayavattanakul M. Hair loss and herbs for treatment. J Cosmet Dermatol 2013;12(3):210-22.
- Upadhyay S, Ghosh AK, Singh V. Hair growth promotant activity of petroleum ether root extract of Glycyrrhiza glabra L (Fabaceae) in female rats. Trop J Pharm 2012;11(5):753-8.
- Tanaka S, Saito M, Tabasa M. Bioassay of crude drug for hair growth promoting activity in mice by a new simple methods. Plant Med Suppl 1980;40:84-90.
- Raja MS, Khan I, Perumal P, Srikakolapu SR, Gotteti SD. Quantitative analysis of glycyrrhizic acid in crude drug and its herbal. Arch Appl Sci Res 2010;3(3):703-6.
- Kang JI, Kim EJ, Kim MK, Jeon YJ, Kang SM, Koh YS, et al. The promoting effect of Ishige sinicola on hair growth. Mar Drugs 2013;11(6):1783-99.
- Nam YJ, Tran LS, Kojima M, Sakakibara H, Nishiyama R, Shin R. Regulatory roles of cytokinins and cytokinin signaling in response to potassium deficiency in Arabidopsis. PLoS One 2012;7(10):e47797.
- Allayie SA, Hemalatha S, Elanohezhiyan C, Manoharan V, Balasubramanian K, Sheikh BA. In vivo evaluation of hair growth potential of fresh leaf extracts of Naringi crenulata. J Clin Exp Dermatol Res 2012;3(2):1-4.
- Sigit H. Pengaruh Ekstrak Etanol Daun Mangkokan (Nothopanx scutellarium L.) Terhadap Kecepatan Pertumbuhan Rambut Kelinci Jantan dan Profil Kromatogram lapis Tipisnya. Thesis. Surakarta: Universitas Muhammadiyah Surakarta; 2005.
- Sadiah S, Herlina N, Indriati D. Effectivity of Ethanol 70% Extract of Mangkokan (Nothopanax scutellarius (Burm. f) Merr) Leaves in Emulsion Form as Hair Growth Stimulant. Fitofarmaka 2014;4(1):10-7.
Cited by
Sorted by citations
Cerulli, A., Masullo, M., Montoro, P., & Piacente, S. (2022) · Cosmetics · doi:10.3390/cosmetics9010007 · Cited by 83 · see citing works
- Authors
- Utami, S. M., Djajadisastra, J., & Saputri, F. C.
- Affiliations
- University of Indonesia (ID)
- Venue
- International Journal of Applied Pharmaceutics
- Publisher
- Innovare Academic Sciences Pvt Ltd
- Published
- 2017-10-30
- Volume
- 9
- Pages
- 44
- ISSN
- 0975-7058 (ISSN-L)
- OpenAlex
- W2767063675
- Cited by
- 12
- References
- 0
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 2
- FWCI
- 0.59
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- International Journal of Applied Pharmaceutics (publishedVersion, cc-by-nc)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacological Effects of Natural Compounds
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- licorice, ethanol extract, hair tonic, physical stability, eye irritation, skin irritation, glycyrrhizin, minoxidil, cosmetic formulation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
