Biotization of tissue culture banana plantlets with Methylobacterium salsuginis to enhance the survival and growth under greenhouse and open environment condition
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Aim: This study aimed to investigate the impact of M. salsuginis TNMB03 biotization on tissue culture banana cv. Grande Naine plantlets growth and survival under greenhouse and open environmental condition (exposed to direct sunlight). Methodology: Banana plantlets were transferred from culture flasks to protray and maintained under the greenhouse and open environmental condition for 30 days with or without M. salsuginis TNMB03 treatment. After 30 days, plant growth parameters like pseudostem height, girth, number of leaves, leaf area, fresh and dry biomass, root parameters, plantlet survival, chlorophyll a and b, total chlorophyll, carotenoids and soluble protein and Methylobacterium population in upper and lower surface of leaf, as well as endophytic population were assessed. Results: This study showed that the plantlets biotized with M. salsuginis TNMB03 had better acclimatization response under both the experimental condition than that of uninoculated plantlets. Positive influence on the survival and growth of M. salsuginis TNMB03 biotized plantlets was observed when transferred directly to greenhouse and open environmental condition. Inoculation of M. salsuginis TNMB03 increased the plant height, girth and number of leaves, root length, lateral root and biomass in comparison to the uninoculated plantlets in greenhouse and open environment. Uninoculated plantlets kept under open environment had lower chlorophyll content and sun scorching damages compared to M. salsuginis TNMB03 inoculated plants, which had dark green leaves and increased chlorophyll content. Interpretation: This study shows a new potential technique of using M. salsuginis TNMB03 in tissue culture plantlets, which can help in enhancing the growth of plantlets transferred from culture vessel to greenhouse or open environmental condition without undergoing the routine acclimatization procedure.
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A significant percentage of in vitro-derived plantlets do not survive when moved from a controlled in vitro environment to ambient glasshouse or outdoor conditions.
“A significant percentage of in vitro-derived plantlets do not survive when moved from a controlled in vitro environment to an ambient glasshouse or outdoor conditions [39,42]”
From Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets (2022) · cites this paper as [42]
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Delayed leaf senescence induces extreme drought tolerance in a flowering plant
Rivero, R. M., Kojima, M., Gepstein, A., Sakakibara, H., Mittler, R., Gepstein, S., & Blumwald, E. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709453104 · Cited by 896 · see citing works
Pirttilä, A. M., Laukkanen, H., Pospiech, H., Myllylä, R., & Hohtola, A. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.7.3073-3077.2000 · Cited by 181 · see citing works
Meena, K. K., Kumar, M., Kalyuzhnaya, M., Yandigeri, M. S., Singh, D. P., Saxena, A. K., & Arora, D. K. (2011) · Antonie van Leeuwenhoek · doi:10.1007/s10482-011-9692-9 · Cited by 173 · see citing works
Knief, C., Francès, L., & Vorholt, J. A. (2010) · Microbial Ecology · doi:10.1007/s00248-010-9725-3 · Cited by 119 · see citing works
Priming for transplant stress resistance in In vitro propagation
Nowak, J., & Shulaev, V. (2003) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1079/ivp2002403 · Cited by 118 · see citing works
The Light Reactions of Photosynthesis
Arnon, D. I. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.11.2883 · Cited by 113 · see citing works
Senthilkumar, M., Madhaiyan, M., Sundaram, S., et al. (2007) · Microbiological Research · doi:10.1016/j.micres.2006.10.007 · Cited by 82 · see citing works
Self-citationKargapolova, K. Y., Burygin, G. L., Tkachenko, O. V., Evseeva, N. V., Pukhalskiy, Y. V., & Belimov, A. A. (2020) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-020-01791-9 · Cited by 51 · see citing works
Cervantes-Martínez, J., López-Díaz, S., & Rodríguez-Garay, B. (2004) · doi:10.1016/j.plantsci.2003.11.029 · Cited by 34
Chernyad’ev, I. I. (2009) · Applied Biochemistry and Microbiology · doi:10.1134/s0003683809040012
A method for detecting methylotrophic bacteria on solid surfaces
Corpe, W. A. (1985) · Journal of Microbiological Methods · doi:10.1016/0167-7012(85)90049-1
An Efficient In vitro Hardening Technique of Tissue Culture Raised Plants
Deb, C. R., & Imchen, T. (2009) · Biotechnology(Faisalabad) · doi:10.3923/biotech.2010.79.83
Elbeltagy, A., Nishioka, K., Suzuki, H., Sato, T., Sato, Y.-I., Morisaki, H., Mitsui, H., & Minamisawa, K. (2000) · Soil Science and Plant Nutrition · doi:10.1080/00380768.2000.10409127
Jie, L., Zifeng, W., Lixiang, C., Hongming, T., Patrik, I., Zide, J., & Shining, Z. (2009) · Biological Control · doi:10.1016/j.biocontrol.2009.08.002
Kalyaeva, M. A., Ivanova, E. G., Doronina, N. V., Zakharchenko, N. S., Trotsenko, Y. A., & Buryanov, Y. I. (2003) · Russian Journal of Plant Physiology · doi:10.1023/a:1023861918193
Krishnamoorthy, R., Kwon, S.-W., Kumutha, K., Senthilkumar, M., Ahmed, S., Sa, T., & Anandham, R. (2018) · 3 Biotech · doi:10.1007/s13205-018-1291-2
Self-citationPlant regeneration from callus and cell suspension cultures of Gerbera jamesonii cv. 'Diablo'
Kumar, S., & Kanwar, J. K. (2005) · European Journal of Horticultural Science · doi:10.1079/ejhs.2005/36485
In vitro Regeneration of Gerbera jamesonii Bolus from Leaf and Petiole Explants
Kumar, S., Kanwar, J. K., & Sharma, D. R. (2004) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/bf03263196
Li, D., Mensah, R. A., Liu, F., Tian, N., Qi, Q., Yeh, K., Xuhan, X., Cheng, C., & Lai, Z. (2019) · Scientia Horticulturae · doi:10.1016/j.scienta.2019.108649
Madhaiyan, M., Reddy, B. V. S., Anandham, R., Senthilkumar, M., Poonguzhali, S., Sundaram, S. P., & Sa, T. (2006) · Current Microbiology · doi:10.1007/s00284-005-0452-9
Self-citationIndole-3-acetic acid production by pink-pigmented facultative methylotrophic bacteria
Omer, Z. S., Tombolini, R., Broberg, A., & Gerhardson, B. (2004) · Plant Growth Regulation · doi:10.1023/b:grow.0000038360.09079.ad
Panigrahi, S., Lakshmi, K. A., Madhuri, V., & Soumya, A. N. (2016) · SpringerBriefs in Applied Sciences and Technology · doi:10.1007/978-981-287-670-6_12
Histological and Biochemical Changes in Aegle marmelos Corr. before and after Acclimatization
Pati, R., Mishra, M., Chandra, R., & Muthukumar, M. (2013) · Tree Genetics and Molecular Breeding · doi:10.5376/tgmb.2013.03.0003
Seasonal variations in location and population structure of endophytes in buds of Scots pine
Pirttila, A. M., Pospiech, H., Laukkanen, H., Myllyla, R., & Hohtola, A. (2005) · Tree Physiology · doi:10.1093/treephys/25.3.289
Poorniammal, R., Senthilkumar, M., Prabhu, S., & Anandhi, K. (2020) · Journal of Pharmacognosy and Phytochemistry · doi:10.22271/phyto.2020.v9.i2ab.11098
Self-citationSenthilkumar, M., Madhaiyan, M., Sundaram, S. P., Sangeetha, H., & Kannaiyan, S. (2008) · Biotechnology Letters · doi:10.1007/s10529-008-9693-6
Self-citationEndophytic microorganisms as potential growth promoters of banana
Ting, A. S. Y., Meon, S., Kadir, J., Radu, S., & Singh, G. (2008) · BioControl · doi:10.1007/s10526-007-9093-1
Huylenbroeck, J. M. V., & Debergh, P. C. (1996) · Physiologia Plantarum · doi:10.1034/j.1399-3054.1996.960220.x
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Research works printed but not linked: 3
- Gamez, R.M., F. Rodríguez, N.M. Vidal, S. Ramirez, R.V. Alvarez, D. Landsman and L. Mariño-Ramírez: Banana (Musa acuminata) transcriptome profiling in response to rhizobacteria: Bacillus amyloliquefaciens Bs006 and Pseudomonas fluorescens Ps006. BMC Genomics, 20, 378. https://doi.org/10.1186/s12864-019- 5763-5 (2019). · DOI
- Madhaiyan, M., S. Poonguzhali, M. Senthilkumar, H. Chung, J. Yang, S.P. Sundaram and T Sa: Growth promotion and induction of systemic resistance in rice cultivar Co-47 (Oryza sativa L.) by Methylobacterium spp. Botanical Studies (Bulletin Acadamia Sinica)., 45, 315-324 (2004).
- O n l i n e C o p y ¨Journal of Environmental Biology, November 2021¨ Madhaiyan, M., S. Poonguzhali, M. Senthilkumar, S.P. Sundaram and T. Sa: Nodulation and plant-growth promotion by methylotrophic bacteria isolated from tropical legumes. Microbiol. Res., 164, 114- 120 (2009).
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Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets
Mohammed, M., Munir, M., & Ghazzawy, H. S. (2022) · Agronomy · doi:10.3390/agronomy13010078 · Cited by 35 · see citing works
- Authors
- Department of Agricultural Microbiology, T. N. A. U., Pushpakanth, P., Krishnamoorthy, R., Department of Crop Management, V. I. O. A., Anandham, R., Department of Agricultural Microbiology, T. N. A. U., Senthilkumar, M., & Department of Agricultural Microbiology, T. N. A. U.
- Affiliations
- Tamil Nadu Agricultural University (IN)
- Corresponding author
- Senthilkumar, M.
- Venue
- Journal of Environmental Biology
- Publisher
- Triveni Enterprises
- Published
- 2021-11-15
- Volume
- 42
- Issue
- 6
- Pages
- 1452-1460
- ISSN
- 0254-8704 (ISSN-L)
- 2394-0379
- OpenAlex
- W3213507692
- Cited by
- 6
- References
- 29
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Environmental Biology (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Banana Cultivation and Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- banana, Grand Naine, plantlet acclimatization, plant growth-promoting bacteria, plantlet survival, chlorophyll content, endophytic bacteria
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