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Identification of vacuolar phosphate influx transporters in Brassica napus
Open accessPreprint — published as 10.1111/pce.14423 · Paper page
Abstract
Recent progress has shown that vacuolar Pi transporters (VPTs) are important for cellular Pi homeostasis against external Pi variations in Arabidopsis and rice, while it is poorly understood for the identity and regulatory mechanism of VPTs in Brassica napus ( B. napus ). Here, we identified two vacuolar Pi influx transporters BnA09PHT5;1b and BnCnPHT5;1b in B. napus and uncovered their necessity for cellular Pi homeostasis through functional analysis. BnPHT5;1bs are the homologs of Arabidopsis AtPHT5;1 with the similar sequence, structure, tonoplast localization, and VPT activity. BnPHT5;1b double mutants had smaller shoot growth and higher shoot cellular Pi than the wild-type B. napus , which are largely different from the report in At PHT5;1 mutant, suggesting PHT5;1-VPTs play a distinct mechanism of cellular Pi homeostasis in seedlings of B. napus and Arabidopsis . By contrast, disruption of BnPHT5;1b genes slowed vegetative growth accompanied by Pi toxicity in floral organs, reduced seed yield and impacted seed traits, agreeing with the role of AtPHT5;1 in floral Pi homeostasis. Taken together, our studies identified two vacuolar Pi influx transporters in B. napus and revealed the distinct and conserved regulatory mechanisms of BnPHT5;1bs in cellular Pi homeostasis in this plant species.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Metadata
- Authors
- Han, B., Wang, C., Wu, T., Yan, J., Jiang, A., Luo, Y., Cai, H., Ding, G., White, P., Liu, Y., Dong, X., XU, F., Wang, S., & Shi, L.
- Affiliations
- Huazhong Agricultural University (CN)
- James Hutton Institute (GB)
- Zhejiang University (CN)
- Innovation Academy for Precision Measurement Science and Technology, CAS (CN)
- Corresponding author
- Wang, Sheliang
- Shi, Lei
- ORCID
- Venue
- Plant Cell & Environment
- Publisher
- Wiley
- Published
- 2022-04-20
- Volume
- 45
- Issue
- 11
- Pages
- 3338-3353
- ISSN
- 0140-7791 (ISSN-L)
- OpenAlex
- W4292543007
- Cited by
- 21
- References
- 1
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 7 · 2023: 5 · 2022: 1
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Plant Cell & Environment (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31972498, 32172662)
- Chinese Academy of Agricultural Sciences
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Nitrogen and Sulfur Effects on Brassica
- Keywords
- Brassica napus, tonoplast, phosphate toxicity, floral organs, seed yield, seed traits, double mutants, Arabidopsis thaliana homologs
- MeSH terms
- Brassica, Phosphates, Plant Proteins, Vacuoles, Arabidopsis, Gene Expression Regulation, Plant, Phosphate Transport Proteins, Brassica napus
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