Identification and Localization of a Possible Rhodopsin in the Echinoderms Asterias forbesi (Asteroidea) and Ophioderma brevispinum (Ophiuroidea)
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Protein extracts of optic cushion tissue from the asteroid Asterias forbesi and arm tissue from the ophiuroid Ophioderma brevispinum were subjected to Western blot analysis. Both tissues contain a membrane-associated protein that reacts with two monoclonal antibodies raised against bovine rhodopsin. This protein migrates slightly behind bovine rhodopsin during sodium-dodecyl-sulfate polyacrylamide gel electrophoresis, suggesting that its molecular weight is slightly larger. Immunohistochemical examination of the optic cushions of A. forbesi revealed a substance that reacts with both monoclonal antibodies; moreover, this substance is more abundant in dark-adapted animals than in light-adapted animals. The arms and central disk of O. brevispinum were also examined immunohistochemically. The tips of the arm spines contain a substance that reacts with both monoclonal antibodies, and at higher magnification this immunoreactive material is localized to small regions within the stroma of the ossicles. Taken together, the biochemical and immunochemical evidence suggests that the cross-reacting protein is homologous to other known rhodopsins and is serving as a visual pigment in echinoderms.
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Experiments using antibodies against bovine rhodopsin indicated the presence of a ciliary-type Opsin in asteroids and ophiuroids.
“experiments using antibodies directed against bovine rhodopsin indicated the presence of a ciliary-type Opsin in asteroids and ophiuroids (Johnsen, 1997).”
From Opsins and clusters of sensory G-protein-coupled receptors in the sea urchin genome (2006) · cites this paper as Johnsen, 1997
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Extraocular Sensitivity to Polarized Light in an Echinoderm
Johnsen, S. (1994) · Journal of Experimental Biology · doi:10.1242/jeb.195.1.281 · Cited by 22 · see citing works
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Opsins and clusters of sensory G-protein-coupled receptors in the sea urchin genome
Raible, F., Tessmar-Raible, K., Arboleda, E., Kaller, T., Bork, P., Arendt, D., & Arnone, M. I. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.08.070 · Cited by 170 · see citing works
- Authors
- Johnsen, S.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Johnsen, S.
- ORCID
- Johnsen, S.
- Venue
- The Biological Bulletin
- Publisher
- University of Chicago Press
- Published
- 1997-08
- Volume
- 193
- Issue
- 1
- Pages
- 97-105
- ISSN
- 0006-3185 (ISSN-L)
- 1939-8697
- OpenAlex
- W2395721109
- Cited by
- 25
- References
- 3
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 1 · 2020: 2 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 3
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-sa
- Locations
- Biological Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Biodiversity Heritage Library (Smithsonian Institution) (submittedVersion, cc-by-nc-sa)
- Funders
- National Science Foundation (9219495)
- University of North Carolina at Chapel Hill
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Keywords
- rhodopsin, Asterias forbesi, arm spines, ossicles, visual pigments, monoclonal antibodies, dark adaptation, protein homology
- MeSH terms
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Epitopes, Cattle, Echinodermata, Immunohistochemistry, Molecular Sequence Data, Rhodopsin, Species Specificity, Photoreceptor Cells, Invertebrate
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