The effects of 20-hydroxyecdysone and juvenile hormone III on tick cells
Abstract
Two cell lines isolated from Rhipicephalus appendiculatus ( RAE 25) and Anocentor (= Dermacentor) nitens (ANE 58) responded to the invertebrate hormones 20-hydroxyecdysone (20-HE) and juvenile hormone III (JH III) in vitro. In the presence of 0.2 or 2 nMolar 20-HE, the cells of the continuous line RAE 25 attached to the culture substrate at a rate of 9% per hr for the first 8 hr, as did cells in growth medium. Twenty or 200 nMolar of 20-HE reduced the rate of cell attachment to 6% per hr, and in the higher hormone concentration the cells ceased to attach after 4 hr. Low concentrations (0.2 and 2 nMolar ) of 20-HE stimulated the growth of the RAE 25 line (P less than 0.02), but 200 nMolar or more inhibited growth (P less than 0.001). Twenty-HE suppressed the growth of the young line ANE 58 in a dose-dependent manner, but the decrease in cell growth was less pronounced than in RAE 25. Ten to 100 times more (2 and 20 mu Molar) 20-HE was needed to achieve significant growth suppression (P less than 0.025 and less than 0.005). The growth of both lines declined (P less than 0.01) by 20% ( RAE 25) or 30% (ANE 58) when the medium contained 38 mu Molar of JH III. The bimodal growth response of line RAE 25 to 20-HE also occurred in the presence of 3.8 and 38 mu Molar JH III, and 2 nMolar 20-HE counteracted the suppressive effect of 38 mu Molar JH III.(ABSTRACT TRUNCATED AT 250 WORDS)
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The same phenomenon (lesser effects at high concentration) has been reported by other authors in different biological systems.
“The same phenomenon has been reported by other authors in different biological systems [88,89].”
From 20-Hydroxyecdysone Confers Antioxidant and Antineoplastic Properties in Human Non-Small Cell Lung Cancer Cells (2023) · cites this paper as [88]
Low 20E concentration enhanced the growth of insect cells and protein synthesis in mice myotubes, whereas high concentration inhibited these processes.
“For instance, it was reported that low 20E concentration enhanced the growth of insect cells [88] and protein synthesis in mice myotubes [89], whereas a high concentration inhibited these processes.”
From 20-Hydroxyecdysone Confers Antioxidant and Antineoplastic Properties in Human Non-Small Cell Lung Cancer Cells (2023) · cites this paper as [88]
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Shuvalov, O., Kirdeeva, Y., Fefilova, E., Netsvetay, S., Zorin, M., Vlasova, Y., Fedorova, O., Daks, A., Parfenyev, S., & Barlev, N. (2023) · Metabolites · doi:10.3390/metabo13050656 · Cited by 21 · see citing works
- Authors
- Kurtti, T. J., & Munderloh, U. G.
- Venue
- The Journal of Parasitology
- Publisher
- JSTOR
- Published
- 1983-12
- Volume
- 69
- Issue
- 6
- Pages
- 1072
- ISSN
- 0022-3395 (ISSN-L)
- OpenAlex
- W2322840621
- Cited by
- 7
- References
- 25
- Citations by year
- 2023: 1 · 2022: 1 · 2015: 1
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Keywords
- 20-hydroxyecdysone, juvenile hormone III, tick cells, Rhipicephalus appendiculatus, Anocentor nitens, cell attachment, cell growth inhibition, dose-response relationship, tick cell lines
- MeSH terms
- Animals, Cell Adhesion, Cell Division, Cell Line, Ecdysterone, Juvenile Hormones, Kinetics, Sesquiterpenes, Ticks
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