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Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay
Abstract
The comet assay, a sensitive method of measuring DNA strand breaks in individual cells, is also capable of identifying apoptotic cells which contain highly fragmented DNA. This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is dependent on both time after irradiation and radiation dose. While some TK6 cells undergo apoptosis within 2 h after irradiation, the fragmentation rate increases approximately 10 h after exposure to radiation doses of 2.5 to 15 Gy. Results confirm that apoptosis is a very rapid event since few cells with intermediate amounts of DNA damage were detected. The comet assay detected apoptotic TK6 cells much earlier than a flow cytometry method.
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Cited by
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.-C., & Sasaki, Y. F. (2000) · Environmental and Molecular Mutagenesis · doi:10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j · Cited by 4,860
An overview of apoptosis assays detecting DNA fragmentation
Majtnerová, P., & Roušar, T. (2018) · Molecular Biology Reports · doi:10.1007/s11033-018-4258-9 · Cited by 330
UVA-induced DNA double-strand breaks result from the repair of clustered oxidative DNA damages
Greinert, R., Volkmer, B., Henning, S., Breitbart, E. W., Greulich, K. O., Cardoso, M. C., & Rapp, A. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks824 · Cited by 146
Wischermann, K., Popp, S., Moshir, S., Scharfetter-Kochanek, K., Wlaschek, M., de Gruijl, F., Hartschuh, W., Greinert, R., Volkmer, B., Faust, A., et al. (2008) · Oncogene · doi:10.1038/onc.2008.70 · Cited by 99
Analysis of DNA damage in individual cells
Olive, P. L., Durand, R. E., Banath, J. P., & Johnston, P. J. (2001) · Methods in cell biology · doi:10.1016/s0091-679x(01)64016-0 · Cited by 77
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Metadata
- Authors
- Olive, P. L., Frazer, G., Banáth, J. P., & Banath, J. P.
- Venue
- Radiation Research
- Publisher
- JSTOR
- Published
- 1993-10
- Volume
- 136
- Issue
- 1
- Pages
- 130
- ISSN
- 0033-7587 (ISSN-L)
- OpenAlex
- W1965281971
- Cited by
- 261
- References
- 0
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay. https://app.cochat.ai/papers/10.2307/3578650
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- Olive, P. L., Frazer, G., Banáth, J. P., & Banath, J. P. (1993). Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay. Radiation Research. https://doi.org/10.2307/3578650
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