The DOI matches its Crossref record; 62 of the 63 references it cites were found in a registry or an index.
Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching.
Open accessAbstract
The photosynthetic apparatus in plants is protected against oxidative damage by processes that dissipate excess absorbed light energy as heat within the light-harvesting complexes. This dissipation of excitation energy is measured as nonphotochemical quenching of chlorophyll fluorescence. Nonphotochemical quenching depends primarily on the [delta]pH that is generated by photosynthetic electron transport, and it is also correlated with the amounts of zeaxanthin and antheraxanthin that are formed from violaxanthin by the operation of the xanthophyll cycle. To perform a genetic dissection of nonphotochemical quenching, we have isolated npq mutants of Chlamydomonas by using a digital video-imaging system. In excessive light, the npq1 mutant is unable to convert violaxanthin to antheraxanthin and zeaxanthin; this reaction is catalyzed by violaxanthin de-epoxidase. The npq2 mutant appears to be defective in zeaxanthin epoxidase activity, because it accumulates zeaxanthin and completely lacks antheraxanthin and violaxanthin under all light conditions. Characterization of these mutants demonstrates that a component of nonphotochemical quenching that develops in vivo in Chlamydomonas depends on the accumulation of zeaxanthin and antheraxanthin via the xanthophyll cycle. However, observation of substantial, rapid, [delta]pH-dependent nonphotochemical quenching in the npq1 mutant demonstrates that the formation of zeaxanthin and antheraxanthin via violaxanthin de-epoxidase activity is not required for all [delta]pH-dependent nonphotochemical quenching in this alga. Furthermore, the xanthophyll cycle is not required for survival of Chlamydomonas in excessive light.
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“The xanthophyll cycle, particularly the de-epoxidation of violaxanthin to zeaxanthin through antheraxanthin, was reported to play an important role in energy dissipation at high light intensity (Demmig-Adams and Adams 1992, Misra et al. 2006).”
From Photosynthesis under stressful environments: An overview (2013) · cites this paper as Niyogi et al. 1997
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Cites
Jeffrey, S. W., & Humphrey, G. F. (1975) · Biochemie und Physiologie der Pflanzen · doi:10.1016/s0015-3796(17)30778-3 · Cited by 5,221
Chlorophyll Fluorescence and Photosynthesis: The Basics
Krause, G. H., & Weis, E. (1991) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.pp.42.060191.001525 · Cited by 4,179
Photoprotection and Other Responses of Plants to High Light Stress
Demmig-Adams, B., & Adams, W. W. (1992) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.pp.43.060192.003123 · Cited by 2,405
Photoinhibition of Photosystem II. Inactivation, protein damage and turnover
Aro, E.-M., Virgin, I., & Andersson, B. (1993) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(93)90134-2 · Cited by 2,366
The use of chlorophyll fluorescence nomenclature in plant stress physiology
van Kooten, O., & Snel, J. F. H. (1990) · Photosynthesis Research · doi:10.1007/bf00033156 · Cited by 2,329
REGULATION OF LIGHT HARVESTING IN GREEN PLANTS
Horton, P., Ruban, A. V., & Walters, R. G. (1996) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.47.1.655 · Cited by 1,768
The role of xanthophyll cycle carotenoids in the protection of photosynthesis
Demmig-Adams, B., & Adams, W. W. (1996) · Trends in Plant Science · doi:10.1016/s1360-1385(96)80019-7 · Cited by 1,673
Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin
Demmig-Adams, B. (1990) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(90)90088-l · Cited by 1,572
Bilger, W., & Bj�rkman, O. (1990) · Photosynthesis Research · doi:10.1007/bf00033159 · Cited by 1,546
Too much of a good thing: light can be bad for photosynthesis
Barber, J., & Andersson, B. (1992) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(92)90503-2 · Cited by 1,014
Cited by
Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo
Baker, N. R. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092759 · Cited by 4,639
“Fluorescence imaging can be used in screening procedures to identify organisms with modified photosynthetic performance, as has been done for algae (11, 88) and Arabidopsis (89) mutants.”
MethodNon-Photochemical Quenching. A Response to Excess Light Energy
Müller, P., Li, X.-P., & Niyogi, K. K. (2001) · Plant Physiology · doi:10.1104/pp.125.4.1558 · Cited by 2,840
“These suggestions were supported by studies on the Chlamydomonas lor1 mutant, which only lacks lutein and loroxanthin, but shows lower qE than the wild type (Niyogi et al., 1997).”
BackgroundResultSelf-citationPhotosynthesis under stressful environments: An overview
Ashraf, M., & Harris, P. J. C. (2013) · Photosynthetica · doi:10.1007/s11099-013-0021-6 · Cited by 2,028
“The dissipation of excitation energy is determined as nonphotochemical quenching (NPQ) of Chl fluorescence during photosynthetic electron transport, which significantly correlates with contents of zeaxanthin and antheraxanthin produced during the xanthophyll cycle (Niyogi et al. 1997).”
BackgroundPHOTOPROTECTION REVISITED:Genetic and Molecular Approaches
Niyogi, K. K. (1999) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.50.1.333 · Cited by 1,933
“For example, Chlamydomonas mutants lacking lutein and loroxanthin ( lor1), antheraxanthin, violaxanthin, and neoxanthin ( npq2), or zeaxanthin ( npq1) are all able to grow as well as wild type in high light (109, 110).”
BackgroundInfluentialSelf-citationThe role of the xanthophyll cycle and of lutein in photoprotection of photosystem II
Jahns, P., & Holzwarth, A. R. (2012) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2011.04.012 · Cited by 1,084
“Other xanthophylls than Zx might thus be involved in NPQ in green algae [36].”
Background
Metadata
- Authors
- Niyogi, K. K., Bjorkman, O., & Grossman, A. R.
- Affiliations
- Carnegie Institution for Science (US)
- Carnegie Institution of Washington, Department of Plant Biology, 290 Panama Street, Stanford, California 94305.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-08-01
- Volume
- 9
- Issue
- 8
- Pages
- 1369-1380
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- PMC
- PMC157004
- OpenAlex
- W1987833932
- Cited by
- 326
- References
- 63
- Citations by year
- 2025: 6 · 2024: 8 · 2023: 7 · 2022: 6 · 2021: 11 · 2020: 14 · 2019: 7 · 2018: 9 · 2017: 8 · 2016: 17 · 2015: 13 · 2014: 14 · 2013: 16 · 2012: 10
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Plant Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Keywords
- Chlamydomonas, xanthophyll cycle, non-photochemical quenching, violaxanthin de-epoxidase, zeaxanthin epoxidase, violaxanthin, antheraxanthin, zeaxanthin, chlorophyll fluorescence quenching, video imaging, photosynthetic electron transport, pH gradient, photoprotection
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- The paper
- Niyogi, K. K., Bjorkman, O., & Grossman, A. R. (1997). Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching. The Plant Cell. https://doi.org/10.1105/tpc.9.8.1369
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