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Cited by 53Bariatric Surgery and OutcomesGut microbiota and healthAdipokines, Inflammation, and Metabolic Diseases

Insulin Resistance, Microbiota, and Fat Distribution Changes by a New Model of Vertical Sleeve Gastrectomy in Obese Rats

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Basso, Nicola, Soricelli, Emanuele, Castagneto-Gissey, Lidia, Casella, Giovanni, Albanese, Davide, Fava, Francesca, Donati, Claudio, Tuohy, Kieran +8 more·2016·Diabetes
DOI 10.2337/db16-0039
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Abstract

Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella. These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.

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  • A different dietary pattern influences β diversity of gut microbiota composition but not α diversity.

    “A different dietary pattern has been shown to influence β diversity of the gut microbiota composition, but not α diversity (Basso et al., 2016), which was quite divergent in individuals on an animal-based diet (David et al., 2014).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Basso et al., 2016

    The Gut Microbiota section reports significant differences in both β-diversity and α-diversity between gastrectomy and sham-operated rats, contrary to the claim that α-diversity was unaffected. The Animals section says both groups received the same high-fat diet, so this was not a comparison of dietary patterns. (LLM, conf=0.87)

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Authors
Basso, N., Soricelli, E., Castagneto-Gissey, L., Casella, G., Albanese, D., Fava, F., Donati, C., Tuohy, K., Angelini, G., La Neve, F., Severino, A., Kamvissi-Lorenz, V., Birkenfeld, A. L., Bornstein, S., Manco, M., & Mingrone, G.
Affiliations
  • Policlinico Umberto I (IT)
  • Surgical-Medical Department for Digestive Diseases, Policlinico Umberto I, University of Rome Sapienza, Rome, Italy
  • Fondazione Edmund Mach (IT)
  • Institute of Cardiology, Catholic University, Rome, Italy
  • Department of Medicine III, Universitätsklinikum Carl Gustav Carus an der Technischen Universität Dresden, Dresden, Germany
  • King's College London (GB)
  • Bambino Gesù Children's Hospital (IT)
Corresponding author
Mingrone, Geltrude
ORCID
Venue
Diabetes
Publisher
American Diabetes Association
Published
2016-07-18
Volume
65
Issue
10
Pages
2990-3001
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
DOI
10.2337/db16-0039
OpenAlex
W2488594835
Cited by
53
References
6
Citations by year
2025: 8 · 2024: 3 · 2023: 3 · 2022: 6 · 2021: 3 · 2020: 13 · 2019: 6 · 2018: 7 · 2017: 4
FWCI
6.28
Type
journal article
Language
en
Open access
Licence
www.diabetesjournals.org/content/license (vor, 66 d)
Locations
  • Diabetes (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
  • Research Portal (King's College London) (submittedVersion)
  • CINECA IRIS Institutional Research Information System (Fondazione Edmund Mach) (submittedVersion, other-oa)
  • Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Bariatric Surgery and Outcomes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
insulin resistance, sleeve gastrectomy, obesity, gut microbiota, body fat distribution, ectopic fat deposition, liver glycogen, ghrelin, glucagon-like peptide-1, Ruminococcus, Lactobacillus, Collinsella, Akt, glycogen synthase kinase-3, AMPK, metabolically healthy obesity, rat model
MeSH terms
Animals, Blood Glucose, Fats, Gastrectomy, Glucose Tolerance Test, Insulin, Insulin Resistance, Lactobacillus, Male, Obesity, Postoperative Care, RNA, Ribosomal, 16S, Blotting, Western, Rats, Wistar, Ruminococcus, Rats, Microbiota

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