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A high‐performance liquid chromatography method for hemoglobin A1c
Abstract
Hemoglobin A1c (HbA1c) is a glycosylated derivative of hemoglobin and is one of a family of derivatives whose concentrations are elevated in patients with diabetes mellitus. Published methods for the measurement of HbA1c are relatively tedious and require modest amounts of blood. A high-performance liquid chromatographic (HPLC) method for the determination of HbA1c is presented. The method is rapid (20 minutes), precise (coefficient of variation of 5-10 per cent), uses small amounts of sample (3 microliter.), can be automated. A sample preparation technique using filtration was developed that shortened and simplified preparation of venous blood and allowed use of capillary samples. HbA1c was measured by this method in three age-stratified groups of controls and a group of insulin-requiring juvenile diabetics. There was clear separation of HbA1c values between all normals (5.9 +/- 1.3, 5.6 +/- 0.7, 7.1 +/- 0.9 per cent) and the diabetics (12.1 +/- 2.4 per cent). Use of this method can facilitate large-scale clinical investigations and permit biochemical investigations of the metabolism and formation of hemoglobin A1c where small sample sizes are necessary.
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“The coefficient of variation of this reference method was < 3%.”
From Twenty‐four‐hour profiles of plasma glucose, insulin, C‐peptide and free fatty acid in subjects with varying degrees of glucose tolerance following short‐term, medium‐dose prednisone (20 mg/day) treatment: evidence for differing effects on insulin secretion and action (2012) · cites this paper as [19]
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DeFronzo, R. A., Ratner, R. E., Han, J., Kim, D. D., Fineman, M. S., & Baron, A. D. (2005) · Diabetes Care · doi:10.2337/diacare.28.5.1092 · Cited by 1,559
Buse, J. B., Henry, R. R., Han, J., Kim, D. D., Fineman, M. S., Baron, A. D., & for the Exenatide-113 Clinical Study Group (2004) · Diabetes Care · doi:10.2337/diacare.27.11.2628 · Cited by 1,346
“HbA1c was measured using a highperformance liquid chromatography methodology (21,22).”
MethodKendall, D. M., Riddle, M. C., Rosenstock, J., Zhuang, D., Kim, D. D., Fineman, M. S., & Baron, A. D. (2005) · Diabetes Care · doi:10.2337/diacare.28.5.1083 · Cited by 1,177
“A1C was measured using a highperformance liquid chromatography methodology (25,26).”
MethodDrucker, D. J., Buse, J. B., Taylor, K., Kendall, D. M., Trautmann, M., Zhuang, D., & Porter, L. (2008) · The Lancet · doi:10.1016/s0140-6736(08)61206-4 · Cited by 1,070
The Glycosylation of Hemoglobin: Relevance to Diabetes Mellitus
Bunn, H. F., Gabbay, K. H., & Gallop, P. M. (1978) · Science · doi:10.1126/science.635569 · Cited by 872
Metadata
- Authors
- Davis, J. E., Mcdonald, J. M., & Jarett, L.
- Affiliations
- Washington University in St. Louis (US)
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1978-02-01
- Volume
- 27
- Issue
- 2
- Pages
- 102-107
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2013055957
- Cited by
- 203
- References
- 0
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 10.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Chromatography
- Keywords
- HbA1c, HPLC assay, diabetes mellitus, type 1 diabetes, capillary blood samples, sample preparation, small sample volumes, automated assay
- MeSH terms
- Adolescent, Adult, Age Factors, Child, Child, Preschool, Chromatography, High Pressure Liquid, Diabetes Mellitus, Diabetes Mellitus, Type 1, Glycosides, Hemoglobins, Humans, Middle Aged
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