Stimulation of Adenylate Cyclase by Ca2+ and Calmodulin in Rat Islets of Langerhans: Explanation for the Glucose-Induced Increase in Cyclic AMP Levels
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The effect of Ca2+ and calmodulin has been studied on adenylate cyclase activity in homogenates of rat islets of Langerhans. EGTA had a stimulatory effect on the enzyme in accord with the known inhibitory effect of Ca2+. In contrast, the addition of Ca2+ together with calmodulin is stimulatory and demonstrates the existence of a Ca2+-dependent adenylate cyclase in islets of Langerhans. It is suggested that the glucose-induced increase in cyclic AMP concentrations in intact islets is a secondary consequence of the glucose-induced increase in cytosol free-Ca2+ concentrations which, with calmodulin, causes an increase in the activity of adenylate cyclase.
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Enhanced AC activity activates protein kinase A (PKA), leading to smooth muscle relaxation.
“Enhanced AC activity activates protein kinase A (PKA), leading to SM relaxation.”
From Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles (2024) · cites this paper as [64]
A relationship exists between increasing cytosolic Ca2+ levels and adenylate cyclase (AC) activity, which involves a Ca2+-dependent enzyme.
“A relationship exists between increasing cytosolic Ca2+ levels and adenylate cyclase (AC) activity, which involves a Ca2+-dependent enzyme [64].”
From Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles (2024) · cites this paper as [64]
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Research works printed but not linked: 4
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“RINm5F cells (40) and rat islets (249) have a calmodulin-sensitive adenylate cyclase, but it is not known if this represents all or just a fraction of the population.”
Self-citation
- Authors
- Sharp, G. W. G., Wiedenkeller, D. E., Kaelin, D., Siegel, E. G., & Wollheim, C. B.
- Affiliations
- University of Geneva (CH)
- ORCID
- Wollheim, Claes B
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1980-01-01
- Volume
- 29
- Issue
- 1
- Pages
- 74-77
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2137242115
- Cited by
- 85
- References
- 0
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2012: 1
- FWCI
- 17.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phosphodiesterase function and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- adenylate cyclase, calcium, calmodulin, pancreatic islets, cyclic AMP, glucose, free calcium, EGTA, enzyme regulation
- MeSH terms
- Cyclic AMP, Adenylyl Cyclases, Animals, Calcium, Calcium-Binding Proteins, Calmodulin, Glucose, Islets of Langerhans, Male, Stimulation, Chemical, Rats
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