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Automated Method for Isolation of Human Pancreatic Islets
Abstract
We describe an automated method for the isolation of human pancreatic islets. The procedure meets the following requirements: 1) minimal traumatic action on the islets, 2) continuous digestion in which the islets that are progressively liberated can be saved from further enzymatic action, 3) minimal human intervention in the digestion process, and 4) high yield and purity of the isolated islets. After purification on Ficoll gradients, an average of 164,600 islets/pancreas was obtained (2279 islets/g), with an average purity of 78.5% islets. The average volume and average insulin content of the final islet preparation were 348 mm3 and 93.4 U, respectively. The islets were morphologically intact with a normal degree of β-granulation and responded to glucose stimulation with a fivefold increase of insulin secretion over basal levels. The procedure is now being used for the initiation of the second phase of clinical trials on human islet transplants.
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Cited by
The unique cytoarchitecture of human pancreatic islets has implications for islet cell function
Cabrera, O., Berman, D. M., Kenyon, N. S., Ricordi, C., Berggren, P.-O., & Caicedo, A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510790103 · Cited by 1,312
Self-citationLong-Term Survival of Xenogeneic Pancreatic Islet Grafts Induced by CTLA4lg
Lenschow, D. J., Zeng, Y., Thistlethwaite, J. R., Montag, A., Brady, W., Gibson, M. G., Linsley, P. S., & Bluestone, J. A. (1992) · Science · doi:10.1126/science.1323143 · Cited by 1,121
Clinical pancreatic islet transplantation
Shapiro, A. M. J., Pokrywczynska, M., & Ricordi, C. (2017) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.178 · Cited by 747
Self-citationDe Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data
Grün, D., Muraro, M. J., Boisset, J.-C., Wiebrands, K., Lyubimova, A., Dharmadhikari, G., van den Born, M., van Es, J., Jansen, E., Clevers, H., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.05.010 · Cited by 586
Moberg, L., Johansson, H., Lukinius, A., Berne, C., Foss, A., Källen, R., Østraat, Ø., Salmela, K., Tibell, A., Tufveson, G., et al. (2002) · The Lancet · doi:10.1016/s0140-6736(02)12020-4 · Cited by 568
Metadata
- Authors
- Ricordi, C., Lacy, P. E., Finke, E. H., Olack, B. J., & Scharp, D. W.
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1988-04-01
- Volume
- 37
- Issue
- 4
- Pages
- 413-420
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2171678230
- Cited by
- 1,310
- References
- 0
- FWCI
- 23.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Automated Method for Isolation of Human Pancreatic Islets. https://app.cochat.ai/papers/10.2337/diab.37.4.413
- The paper
- Ricordi, C., Lacy, P. E., Finke, E. H., Olack, B. J., & Scharp, D. W. (1988). Automated Method for Isolation of Human Pancreatic Islets. Diabetes. https://doi.org/10.2337/diab.37.4.413
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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