Cited by 50Metabolism, Diabetes, and CancerPancreatic function and diabetesGlycogen Storage Diseases and Myoclonus

Human Hexokinase II mRNA and Gene Structure

Printz, Richard L, Ardehali, Hossein, Koch, Stephen, Granner, Daryl K·1995·Diabetes

Abstract

This study reports the isolation and characterization of the human hexokinase II (HKII) gene. This gene is ∼ 50 kilobases in length and contains 18 exons, ranging in size from 96 to 2,536 base pairs, that are exactly the same size as the corresponding exons in the rat HKII gene. A cDNA representing the entire open reading frame for HKII was synthesized using a series of polymerase chain reactions with human skeletal muscle RNA as the template, and this allowed us to deduce the complete structure of the HKII mRNA. The human HKII mRNA has 431 nucleotides (nt) of 5′ noncoding sequence, 2,751 nt of coding sequence, and 2,394 nt of 3′ noncoding sequence. The open reading frame encodes a protein of 917 amino acids with an estimated molecular mass of 102.4 kDa. There is a high degree of similarity in the amino acid and nt sequences of the rat and human glucokinase and HKII proteins and genes. This, coupled with the observation that the exon sizes are conserved, suggests a common evolutionary origin of the these two genes.

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Crossref · 10.2337/diab.44.3.290

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  • Hexokinase isoenzymes HK-I-IV catalyze the first committed step of glucose metabolism, converting free glucose to glucose-6-phosphate.

    “Hexokinase isoenzymes (HK-I–IV) catalyze the first committed step of glucose metabolism, the intracellular conversion of free glucose to glucose-6-phosphate (Glu-6-P) [183, 184, 186, 198].”

    From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [198]

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Authors
Printz, R. L., Ardehali, H., Koch, S., & Granner, D. K.
Affiliations
Vanderbilt University (US)
Venue
Diabetes
Published
1995-03-01
Volume
44
Issue
3
Pages
290-294
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
OpenAlex
W2102167739
Cited by
50
References
0
Citations by year
2022: 1 · 2021: 1 · 2019: 1 · 2018: 2 · 2016: 1 · 2015: 2 · 2012: 1
FWCI
2.73
Type
journal article
Language
en
Open access
closed
Locations
  • Diabetes (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01dk046867-09)
  • Vanderbilt Diabetes Research and Training Center, Vanderbilt University Medical Center
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-46867, DK42502, DK35107)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Health Sciences › Medicine › Rheumatology › Glycogen Storage Diseases and Myoclonus
Keywords
hexokinase 2, mRNA structure, exon organization, cDNA cloning, glucokinase, gene evolution
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Glucokinase, Hexokinase, Humans, Introns, Isoenzymes, Molecular Sequence Data, Muscles, RNA, Messenger, Templates, Genetic, Restriction Mapping, Hominidae, Polymerase Chain Reaction, Open Reading Frames, Sequence Homology, Amino Acid, Rats

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