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Cited by 212Adipose Tissue and MetabolismLipid metabolism and biosynthesisPeroxisome Proliferator-Activated Receptors

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

Mice with Targeted Disruption of the Dio2 Gene Have Cold-Induced Overexpression of the Uncoupling Protein 1 Gene but Fail to Increase Brown Adipose Tissue Lipogenesis and Adaptive Thermogenesis

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Christoffolete, Marcelo A., Linardi, Camila C.G., de Jesus, Lucia, Ebina, Katia Naomi, Carvalho, Suzy D., Ribeiro, Miriam O., Rabelo, Rogerio, Curcio, Cyntia, Martins, Luciane, Kimura, Edna T., Bianco, Antonio C.·2004·Diabetes
DOI 10.2337/diabetes.53.3.577
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Abstract

The Dio2 gene encodes the type 2 deiodinase (D2) that activates thyroxine (T4) to 3,3′,5-triiodothyronine (T3), the disruption of which (Dio2−/−) results in brown adipose tissue (BAT)-specific hypothyroidism in an otherwise euthyroid animal. In the present studies, cold exposure increased Dio2−/− BAT sympathetic stimulation ∼10-fold (normal ∼4-fold); as a result, lipolysis, as well as the mRNA levels of uncoupling protein 1, guanosine monophosphate reductase, and peroxisome proliferator–activated receptor γ coactivator 1, increased well above the levels detected in the cold-exposed wild-type animals. The sustained Dio2−/− BAT adrenergic hyperresponse suppressed the three- to fourfold stimulation of BAT lipogenesis normally seen after 24–48 h in the cold. Pharmacological suppression of lipogenesis with ββ′-methyl-substituted α-ω-dicarboxylic acids of C14–C18 in wild-type animals also impaired adaptive thermogenesis in the BAT. These data constitute the first evidence that reduced adrenergic responsiveness does not limit cold-induced adaptive thermogenesis. Instead, the resulting compensatory hyperadrenergic stimulation prevents the otherwise normal stimulation in BAT lipogenesis during cold exposure, rapidly exhausting the availability of fatty acids. The latter is the preponderant determinant of the impaired adaptive thermogenesis and hypothermia in cold-exposed Dio2−/− mice.

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  1. Relative quantitation of gene expression. In User Bulletin #2. Foster City, CA, Applied Biosystems/Perkin-Elmer Co.,1997, p.1–36

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Metadata

Authors
Christoffolete, M. A., Linardi, C. C. G., de Jesus, L., Ebina, K. N., Carvalho, S. D., Ribeiro, M. O., Rabelo, R., Curcio, C., Martins, L., Kimura, E. T., & Bianco, A. C.
Affiliations
  • Brigham and Women's Hospital (US)
  • Universidade de São Paulo (BR)
  • Department of Medicine, Thyroid Section, Division of Endocrinology, Diabetes and Hypertension, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts
  • Department of Histology & Embryology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
  • Department of Physiology & Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
  • Universidade Presbiteriana Mackenzie (BR)
ORCID
Venue
Diabetes
Publisher
American Diabetes Association
Published
2004-03-01
Volume
53
Issue
3
Pages
577-584
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
DOI
10.2337/diabetes.53.3.577
OpenAlex
W2155560632
Cited by
212
References
40
Citations by year
2026: 1 · 2025: 6 · 2024: 7 · 2023: 10 · 2022: 13 · 2021: 11 · 2020: 11 · 2019: 11 · 2018: 9 · 2017: 13 · 2016: 11 · 2015: 13 · 2014: 19 · 2013: 12 · 2012: 6
FWCI
5.15
Type
journal article
Language
en
Open access
Locations
  • Diabetes (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Thyroid Association
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (97/13888-5)
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK36256, R01DK036256)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
DIO2, type 2 deiodinase, brown adipose tissue, adaptive thermogenesis, UCP1, lipogenesis, cold exposure, sympathetic nervous system, hypothyroidism, thyroxine, fatty acid availability, hypothermia
MeSH terms
Uncoupling Protein 1, Iodothyronine Deiodinase Type II, Animals, Base Sequence, Body Temperature Regulation, Adipose Tissue, Brown, Carrier Proteins, Gene Expression Regulation, Hypothyroidism, Iodide Peroxidase, Ion Channels, Lipids, Membrane Proteins, Transcription Factors, Polymerase Chain Reaction, DNA Primers, Receptors, Cytoplasmic and Nuclear, Mice, Knockout, Mitochondrial Proteins, Mice

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