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Cited by 5Plant tissue culture and regenerationFlowering Plant Growth and CultivationGrowth and nutrition in plants

In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method

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Radomir, Ana-Maria, Stan, Ramona, Pandelea, Mariana Letiția, Vizitiu, Diana Elena·2022·Acta Scientiarum Polonorum Hortorum Cultus
DOI 10.24326/asphc.2022.4.5
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Abstract

The aim of this study was to elaborate an efficient in vitro multiplication protocol for Mentha piperita L. (peppermint) and to perform a comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method. The use of a plain Murashige and Skoog (MS) basal medium favored the induction of regenerative processes, the percentage of explants that started to grow four weeks after inoculation being 92%. The highest multiplication rate (7.12 shoots/explant) and the highest average shoot length (8.11 cm) were obtained on the MS medium supplemented with 1 mg/L benzylaminopurine, when nodal fragments were used as explants. The rooting phase was not necessary, the shoots developing roots on the multiplication medium. The acclimatization rate of in vitro regenerated plants to ex vitro conditions was 96%. Although biochemical investigations revealed some differences between in vitro regenerated plants and those obtained by conventional methods, the results obtained show that micropropagation can be used successfully to obtain high-quality peppermint biological material, a potential source of bioactive compounds with therapeutic effect.

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  • In Mentha piperita, the content of photosynthetic pigments in plants regenerated in vitro was higher than in seed-generated plants, while total phenol content was higher in plants obtained by classical methods than in in vitro ones.

    “Radomir et al., 2022 [143], compared a number of biochemical indicators in in vitro- and seed-generated Mentha piperita L. plants and identified some differences between the two types of regenerants: the content of photosynthetic pigments in the plants regenerated in vitro was higher than in the ones obtained from seeds, while the total phenol content in the plants obtained by classical methods”

    From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as Radomir et al., 2022 [143]

    The Abstract (page not provided) says the study compared biochemical compounds in micropropagated and conventionally obtained peppermint and found some differences. The available passages do not say that the conventional plants were seed-generated or establish which group had higher photosynthetic pigments or total phenols; searches for those comparisons found no results passage. (LLM, conf=0.78)

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Cited by

Sorted by citations

Authors
Radomir, A.-M., Stan, R., Pandelea, M. L., & Vizitiu, D. E.
ORCID
Venue
Acta Scientiarum Polonorum Hortorum Cultus
Publisher
Uniwersytet Przyrodniczy w Lublinie
Published
2022-08-31
Volume
21
Issue
4
Pages
45-52
ISSN
  • 1644-0692 (ISSN-L)
  • 2545-1405
DOI
10.24326/asphc.2022.4.5
OpenAlex
W4293570611
Cited by
5
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2
FWCI
0.33
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta Scientiarum Polonorum Hortorum Cultus (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
Keywords
peppermint, micropropagation, benzylaminopurine, shoot multiplication, acclimatization, biochemicals, bioactive compounds, nodal explants

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