IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils-fibroblasts interaction in psoriasis
Abstract
Purpose Psoriasis is a common skin disease affecting about 1-3% of the world population. Many types of cells, including lymphocytes, dendritics APCs (antigen presenting cells), NKT (natural killer T) cells, neutrophils, keratinocytes and fibroblasts are involved in the pathogenesis of psoriasis.The aim of our study was to assess in psoriatic patients the production of IL-8, IL-10 and IL-12 cytokines by neutrophils, fibroblasts and fibroblasts - neutrophils interaction.Material/methods The production of IL-8, IL-10 and IL-12 cytokines was evaluated in supernatants after cells incubation for 21 h in culture medium alone and in medium in the presence of IL-8 or TNF-α. Concentrations of IL-8, IL-10, IL-12 were measured by enzyme-linked immunosorbent assay (ELISA) method using commercially available kits.Results Our results demonstrate that fibroblasts are not able to produce IL-10 and IL-12 but they generate IL-8. The amount of IL-8 depends on the site of derivation of fibroblasts and on the stimuli. Neutrophils released IL-8, IL-12 (at a lower level in psoriatic patients) and IL-10 but only in the case of healthy donors and at a very low concentration. Moreover, we observed higher concentrations of IL-12 and IL-8 in supernatants as a result of fibroblasts-neutrophils interaction in psoriatic patients.Conclusions Our results suggest that fibroblasts take part in the inflammatory reaction in psoriasis via cytokines or direct interaction with neutrophils. Fibroblasts probably do not exert any anti-inflammatory effect.
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TGF-β and IL-8 have been reported to be secreted by activated neutrophils.
“Both of these have also been reported to be secreted by activated neutrophils [82, 83].”
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The Immunology of Psoriasis-Current Concepts in Pathogenesis
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- Authors
- Glowacka, E., Lewkowicz, P., Rotsztejn, H., & Zalewska, A.
- Affiliations
- Polish Mother’s Memorial Hospital Research Institute (PL)
- Medical University of Lodz (PL)
- Corresponding author
- Rotsztejn, H
- Venue
- Advances in Medical Sciences
- Publisher
- Elsevier BV
- Published
- 2010
- Volume
- 55
- Issue
- 2
- Pages
- 254-260
- ISSN
- 0067-6489 (ISSN-L)
- 1896-1126
- OpenAlex
- W2014613937
- Cited by
- 60
- References
- 37
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 9 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 6 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 7 · 2012: 5
- FWCI
- 1.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Advances in Medical Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Scientific Council in Warsaw (no 4PO5A 07918)
- Medical University of Lodz (no 502-11-726 and no 503-1019-1)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Rheumatology › Spondyloarthritis Studies and Treatments
- Keywords
- psoriasis, interleukin-8, IL-12, IL-10, neutrophils, fibroblasts, cytokine production, TNF-α, skin inflammation
- MeSH terms
- Adult, Female, Fibroblasts, Humans, Male, Middle Aged, Neutrophils, Psoriasis, Interleukin-8, Interleukin-10, Interleukin-12
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