0678 The effect of two microbial inoculants on the aerobic stability of high-moisture corn
Abstract
The objective of this study was to evaluate the effect of two microbial inoculants on the fermentation characteristics and aerobic stability of high-moisture corn (HMC). High-moisture corn (71% DM) was 1) untreated (control) or 2) treated with Lactobacillus hilgardii (LH) (600,000 cfu/g of fresh material), 3) treated with Lactobacillus buchneri 40788 (600,000 cfu/g), or 4) treated with L. hilgardii + L. buchneri 40788 (LH+LB) (300,000 cfu/g). Inoculants were supplied by Lallemand Animal Nutrition, Milwaukee, WI. Five individually replicated lab silos (7.5 L) for each treatment were packed (packing density of 669 kg DM/m3) and ensiled for 10, 30, and 92 d between 21 and 23°C. Data were analyzed using the Fit Model in JMP (SAS Inst. Inc., Cary, NC) as a 4 × 3 factorial arrangement of treatments with the main effects of treatment, day, and their interaction. The numbers of lactic acid bacteria were greater for LH (8.91 log cfu/g) than the control (8.41 log cfu/g) at 30 d, whereas all inoculated treatments had higher numbers (7.91 to 8.03 log cfu/g) than the control (7.18 log cfu/g) at 92 d (P < 0.01). At 10 d, all treated HMC had lower numbers of yeasts (3.53 to 4.18 log cfu/g) than the control (5.68 log cfu/g). The same occurred at 30 d (<2.00 to 2.26 vs. 5.18 log cfu/g) (P < 0.01). At 92 d, LH (4.30 log cfu/g) was numerically lower than the control (4.91 log cfu/g) and LH+LB and LB (both < 2.00 log cfu/g) had lower numbers of yeasts (P < 0.01). There were lower (P < 0.01) concentrations of lactic acid for treated HMC compared with the control at both 30 (0.74 to 0.80 vs. 1.04%) and 92 d (0.68 to 1.15 vs. 1.45%). All treated HMC had higher (P < 0.01) concentrations of acetic acid than the control at 30 (0.47 to 0.57 vs. 0.13%) and 92 d (0.98 to 1.16 vs. 0.23%). Concentrations of ethanol for treated HMC were lower (P < 0.01) than the control at 92 d (0.39 to 0.48 vs. 0.73%). All inoculated HMC were more (P < 0.01) aerobically stable than the control at 10 (42 to 56 vs. 22 h), 30 (>250 vs. 31 h), and 92 d (>250 vs. 49 h). The inoculants used in this study altered the fermentation characteristics and improved the aerobic stability of HMC.
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After 10 d of fermentation, aerobic stability was 22 h for untreated silage, 42 h with Lb. buchneri, and 56 h with Lb. hilgardii.
“After 10 d of fermentation the aerobic stability was 22 h for untreated silage, whereas it increased to 42 h with Lb. buchneri and to 56 h with Lb. hilgardii.”
From Silage review: Recent advances and future uses of silage additives (2018) · cites this paper as Polukis et al., 2016
Both Lb. hilgardii and Lb. buchneri resulted in silages that were stable for more than 250 h after 30 and 90 d of fermentation compared with 31 to 49 h of stability for untreated silage.
“Both organisms resulted in silages that were stable for more than 250 h after 30 and 90 d of fermentation compared with 31 to 49 h of stability for untreated silage.”
From Silage review: Recent advances and future uses of silage additives (2018) · cites this paper as Polukis et al., 2016
Addition of Lb. hilgardii CNCM I-4785 or Lb. buchneri 40788 to high-moisture corn resulted in similar increases in production of acetic acid and 1,2-propanediol and lower numbers of yeasts compared with untreated corn silage.
“Polukis et al. (2016) added Lb. hilgardii CNCM I-4785 or Lb.buchneri 40788 to high-moisture corn at a final application rate of 6 × 10 5 cfu/g of fresh high-moisture corn.They reported that both organisms resulted in similar increases in production of acetic acid and 1,2-propanediol and lower numbers of yeasts when compared with untreated corn silage.”
From Silage review: Recent advances and future uses of silage additives (2018) · cites this paper as Polukis et al., 2016
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Silage review: Recent advances and future uses of silage additives
Muck, R. E., Nadeau, E. M. G., McAllister, T. A., Contreras-Govea, F. E., Santos, M. C., & Kung, L. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-13839 · Cited by 1,038 · see citing works
Self-citation
- Authors
- Polukis, S. A., Smith, M. L., Savage, R. M., Benjamim da Silva, E., Laubach, A. E., Gray, A. M., & Kung, L.
- Affiliations
- University of Delaware (US)
- Venue
- Journal of Animal Science
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-10-01
- Volume
- 94
- Issue
- suppl_5
- Pages
- 324-324
- ISSN
- 0021-8812 (ISSN-L)
- 1525-3163
- OpenAlex
- W2555376394
- Cited by
- 3
- References
- 0
- Citations by year
- 2020: 2 · 2018: 1
- Type
- conference abstract
- Language
- en
- Open access
- closed
- Locations
- Journal of Animal Science (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- high-moisture corn, microbial inoculants, aerobic stability, Lactobacillus hilgardii, Lactobacillus buchneri, silage fermentation, lactic acid bacteria, yeast count, silage quality, corn silage, volatile fatty acids, ammonia nitrogen, lactic acid, acetic acid
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