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Cited by 8RNA Research and SplicingDevelopmental Biology and Gene RegulationRNA and protein synthesis mechanisms

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Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26

Open access
Salerno-Kochan, Anna, Horn, Andreas, Ghosh, Pritha, Nithin, Chandran, Kościelniak, Anna, Meindl, Andreas, Strauss, Daniela, Krutyhołowa, Rościsław, Rossbach, Oliver, Bujnicki, Janusz M, Gaik, Monika, Medenbach, Jan, Glatt, Sebastian·2022·Life Science Alliance
DOI 10.26508/lsa.202201418PMC PMC9070667

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Abstract

The TRIM-NHL protein Meiotic P26 (Mei-P26) acts as a regulator of cell fate in Drosophila . Its activity is critical for ovarian germline stem cell maintenance, differentiation of oocytes, and spermatogenesis. Mei-P26 functions as a post-transcriptional regulator of gene expression; however, the molecular details of how its NHL domain selectively recognizes and regulates its mRNA targets have remained elusive. Here, we present the crystal structure of the Mei-P26 NHL domain at 1.6 Å resolution and identify key amino acids that confer substrate specificity and distinguish Mei-P26 from closely related TRIM-NHL proteins. Furthermore, we identify mRNA targets of Mei-P26 in cultured Drosophila cells and show that Mei-P26 can act as either a repressor or activator of gene expression on different RNA targets. Our work reveals the molecular basis of RNA recognition by Mei-P26 and the fundamental functional differences between otherwise very similar TRIM-NHL proteins.

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  1. 10.1016/j.molcel.2020.03.011 · DOI
  2. 10.1016/j.cell.2012.04.031 · DOI · Paper page
  3. 10.1038/s41467-018-03920-7 · DOI
  4. 10.1038/nsmb.2638 · DOI
  5. 10.1093/nar/gks1032 · DOI
  6. 10.1016/j.celrep.2015.09.068 · DOI
  7. 10.1139/bcb-76-2-3-351 · DOI
  8. 10.1016/0968-0004(92)90308-V · DOI
  9. 10.1093/emboj/20.9.2140 · DOI · Paper page
  10. 10.1016/S0968-0004(98)01299-7 · DOI · Paper page
  11. 10.1515/hsz-2019-0158 · DOI
  12. 10.1101/gad.291377.116 · DOI
  13. 10.1016/j.tibs.2010.04.003 · DOI
  14. 10.1016/j.semcdb.2015.10.017 · DOI
  15. 10.1371/journal.pgen.1004797 · DOI
  16. 10.1016/j.stem.2012.08.012 · DOI
  17. 10.1242/dev.077412 · DOI
  18. 10.1371/journal.pone.0058301 · DOI
  19. 10.1093/genetics/155.4.1757 · DOI
  20. 10.1083/jcb.201407102 · DOI
  21. 10.1038/nature07014 · DOI
  22. 10.1038/sj.onc.1203706 · DOI · Paper page
  23. 10.1101/gad.870801 · DOI · Paper page
  24. 10.1016/j.cub.2006.08.093 · DOI
  25. 10.1016/j.devcel.2010.11.019 · DOI
  26. 10.1016/j.devcel.2006.09.009 · DOI · Paper page
  27. 10.1523/JNEUROSCI.5092-07.2008 · DOI
  28. 10.1101/gad.1119403 · DOI
  29. 10.1093/nar/gkab635 · DOI
  30. 10.1073/pnas.1120473109 · DOI
  31. 10.1242/dev.182063 · DOI
  32. 10.1101/gad.236513.113 · DOI
  33. 10.1091/mbc.e20-07-0453 · DOI
  34. 10.7554/eLife.52358 · DOI
  35. 10.1016/j.ymeth.2016.12.003 · DOI
  36. 10.1016/j.sbi.2016.05.005 · DOI
  37. 10.1016/j.ymeth.2019.10.003 · DOI
  38. 10.1016/j.cell.2011.05.005 · DOI · Paper page
  39. 10.1093/nar/gky1284 · DOI
  40. 10.1073/pnas.1603715113 · DOI
  41. 10.1186/s13059-015-0687-0 · DOI
  42. 10.1101/gr.112961.110 · DOI
  43. 10.1101/2021.10.04.463034 · DOI · Paper page
  44. 10.1371/journal.pone.0030445 · DOI
  45. 10.1016/j.cell.2008.12.024 · DOI
  46. 10.1111/j.1742-4658.2005.04653.x · DOI
  47. 10.1002/bies.201500033 · DOI
  48. 10.1073/pnas.0901452106 · DOI
  49. 10.1534/genetics.109.100693 · DOI
  50. 10.1093/genetics/109.3.529 · DOI
  51. 10.1126/science.1208146 · DOI
  52. 10.1093/genetics/117.2.221 · DOI
  53. 10.3389/fmicb.2016.00496 · DOI
  54. 10.1242/dev.124.21.4361 · DOI
  55. 10.1016/j.celrep.2015.10.017 · DOI
  56. 10.1093/emboj/cdg539 · DOI
  57. 10.1101/gad.371906 · DOI
  58. 10.1101/gad.371406 · DOI
  59. 10.1038/nature13693 · DOI
  60. 10.1016/j.cell.2006.01.038 · DOI
  61. 10.1534/genetics.114.167502 · DOI
  62. 10.1242/dev.129.2.399 · DOI
  63. 10.1038/ncb1223 · DOI
  64. 10.1107/S0907444909047337 · DOI · Paper page
  65. 10.1107/S0021889807021206 · DOI · Paper page
  66. 10.1107/S0907444904019158 · DOI · Paper page
  67. 10.1107/S0907444912001308 · DOI · Paper page
  68. DeLano, 2002, Pymol: An open-source molecular graphics tool, CCP4 Newsl Protein Crystallogr, 40, 82
  69. 10.1002/jcc.20084 · DOI
  70. 10.1002/wcms.1121 · DOI
  71. 10.1063/1.445869 · DOI
  72. 10.1016/j.physbeh.2017.03.040 · DOI · Paper page
  73. 10.1021/ct100481h · DOI
  74. 10.1021/ct200162x · DOI
  75. 10.1063/1.470117 · DOI
  76. 10.1021/ct400314y · DOI
  77. 10.1021/ct200909j · DOI
  78. 10.1016/j.cpc.2012.09.022 · DOI
  79. 10.1093/protein/9.11.1063 · DOI
  80. 10.1093/bioinformatics/bts243 · DOI
  81. 10.1089/adt.2011.0380 · DOI
  82. 10.1101/2021.08.15.456425 · DOI · Paper page
  83. 10.1093/bioinformatics/btab184 · DOI
  84. 10.1002/prot.21123 · DOI

Metadata

Authors
Salerno-Kochan, A., Horn, A., Ghosh, P., Nithin, C., Kościelniak, A., Meindl, A., Strauss, D., Krutyhołowa, R., Rossbach, O., Bujnicki, J. M., Gaik, M., Medenbach, J., & Glatt, S.
Affiliations
  • Jagiellonian University (PL)
  • University of Regensburg (DE)
  • International Institute of Molecular and Cell Biology (PL)
  • Justus-Liebig-Universität Gießen (DE)
Corresponding author
  • Gaik, Monika
  • Medenbach, Jan
  • Glatt, Sebastian
ORCID
Venue
Life Science Alliance
Publisher
Life Science Alliance, LLC
Published
2022-05-05
Volume
5
Issue
8
Pages
e202201418
ISSN
2575-1077 (ISSN-L)
DOI
10.26508/lsa.202201418
PMC
PMC9070667
OpenAlex
W4229075545
Cited by
8
References
1
Citations by year
2026: 4 · 2025: 1 · 2024: 1 · 2022: 2
FWCI
0.33
Type
journal article
Language
en
Open access
Licence
Locations
  • Life Science Alliance (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Jagiellonian University Repository (Jagiellonian University) (submittedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • European Union’s Horizon 2020 research and innovation programme (665778)
  • National Science Centre (UMO-2015/19/P/NZ1/02514, UMO-2019/32/T/NZ1/00420, 2017/26/A/NZ1/01083)
  • German Research Foundation (SFB960 TP B11, ME4238/1-1, RTG2355 325443116)
  • German Federal Ministry of Education and Research (01ZX1401D)
  • Foundation for Polish Science (TECH CORE FACILITY/2017-4/6, TEAM/2016-3/18)
  • IIMCB Statutory Funds
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
RNA recognition, post-transcriptional regulation, mRNA targets, substrate specificity, crystal structure, Drosophila, germline stem cells, oocyte differentiation, spermatogenesis, cell fate regulation, RNA-binding proteins
MeSH terms
Tripartite Motif Proteins, Animals, Drosophila, Male, RNA, RNA, Messenger, Drosophila Proteins

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