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Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013
Open accessAbstract
We describe a novel spike pseudoparticle neutralisation assay (ppNT) for seroepidemiological studies on Middle East respiratory syndrome coronavirus (MERS-CoV) and apply this assay together with conventional microneutralisation (MN) tests to investigate 1,343 human and 625 animal sera. The sera were collected in Egypt as a region adjacent to areas where MERS has been described, and in Hong Kong, China as a control region. Sera from dromedary camels had a high prevalence of antibody reactive to MERS-CoV by MERS NT (93.6%) and MERS ppNT (98.2%) assay. The antibody titres ranged up to 1,280 and higher in MN assays and 10,240 and higher in ppNT assays. No other investigated species had any antibody reactivity to MERS-CoV. While seropositivity does not exclude the possibility of infection with a closely related virus, our data highlight the need to attempt detection of MERS-CoV or related coronaviruses in dromedary camels. The data show excellent correlation between the conventional MN assay and the novel ppNT assay. The newly developed ppNT assay does not require Biosafety Level 3 containment and is thus a relatively high-throughput assay, well suited for large-scale seroepidemiology studies which are needed to better understand the ecology and epidemiology of MERS-CoV.
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Cited by
Tang, X.-C., Agnihothram, S. S., Jiao, Y., Stanhope, J., Graham, R. L., Peterson, E. C., Avnir, Y., Tallarico, A. S. C., Sheehan, J., Zhu, Q., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1402074111 · Cited by 232
MERS-CoV Antibody Responses 1 Year after Symptom Onset, South Korea, 2015
Choe, P. G., Perera, R. A. P. M., Park, W. B., Song, K.-H., Bang, J. H., Kim, E. S., Kim, H. B., Ko, L. W. R., Park, S. W., Kim, N.-J., et al. (2017) · Emerging Infectious Diseases · doi:10.3201/eid2307.170310 · Cited by 227
Self-citationZhao, J., Perera, R. A. P. M., Kayali, G., Meyerholz, D., Perlman, S., & Peiris, M. (2015) · Journal of Virology · doi:10.1128/jvi.00446-15 · Cited by 68
Self-citationGlobal Diversification and Distribution of Coronaviruses With Furin Cleavage Sites
Liu, X., Wu, Q., & Zhang, Z. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.649314 · Cited by 32
Metadata
- Authors
- Perera, R. A., Wang, P., Gomaa, M. R., El-Shesheny, R., Kandeil, A., Bagato, O., Siu, L. Y., Shehata, M. M., Kayed, A. S., Moatasim, Y., Li, M., Poon, L. L., Guan, Y., Webby, R. J., Ali, M. A., Peiris, J. S., & Kayali, G.
- Venue
- Eurosurveillance
- Published
- 2013-09-05
- Volume
- 18
- Issue
- 36
- Pages
- pii=20574-pii=20574
- ISSN
- 1025-496X (ISSN-L)
- 1560-7917
- OpenAlex
- W2022439931
- Cited by
- 323
- References
- 0
- FWCI
- 35.45
- Type
- journal article
- Language
- en
- Open access
- diamond
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- The paper
- Perera, R. A., Wang, P., Gomaa, M. R., El-Shesheny, R., Kandeil, A., Bagato, O., Siu, L. Y., Shehata, M. M., Kayed, A. S., Moatasim, Y., Li, M., Poon, L. L., Guan, Y., Webby, R. J., Ali, M. A., Peiris, J. S., & Kayali, G. (2013). Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013. Eurosurveillance. https://doi.org/10.2807/1560-7917.es2013.18.36.20574
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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