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Cited by 22Neonatal Respiratory Health ResearchTissue Engineering and Regenerative MedicineTransplantation: Methods and Outcomes

The Effect of Culture Conditions on Cytodifferentiation of Fetal Mouse Lung Respiratory Passageways

Hilfer, S. Robert, Schneck, Stephanie L., Brown, Joyce W.·1986·Experimental Lung Research
DOI 10.3109/01902148609061488
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Abstract

Differentiation of the respiratory region of fetal mouse lungs was investigated in serum-free medium supplemented with growth factors and hormones. Terminal buds from the margins of a lobe were removed from 16-day fetuses and organ cultures prepared either in submersion culture or at the air-medium interface. It was found that glycyl-L-histidyl-L-lysine, transferrin, and somatostatin were sufficient to promote branching in the absence of serum. However, type II pneumocytes containing lamellar bodies formed only in the presence of thyroxine or dexamethasone. At concentrations of these hormones slightly above the physiological range most of the cells became cuboidal and contained lamellar bodies; at lower concentrations regions of flattened cells appeared. In submersion culture a large, central cavity surrounded by saccules was formed rather than a branched tree. Thus, the pattern of differentiation is significantly influenced by culture conditions.

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Crossref · 10.3109/01902148609061488

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26 of 26 references verified0 citation errors · 0 not checkable

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  • A mixture of GHK, transferrin, and somatostatin was sufficient to promote branching in serum-free organ culture.

    “The mixture of GHK, transferrin, and somatostatin was sufficient to promote branching in the absence of serum in organ culture, all of which could be added to the misting solution [64].”

    From GHK and DNA: Resetting the human genome to health (2014) · cites this paper as [64]

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Authors
Hilfer, S. R., Schneck, S. L., & Brown, J. W.
Affiliations
Temple University (US)
Venue
Experimental Lung Research
Publisher
Informa UK Limited
Published
1986-01
Volume
10
Issue
2
Pages
115-136
ISSN
  • 0190-2148 (ISSN-L)
  • 1521-0499
DOI
10.3109/01902148609061488
OpenAlex
W1965602311
Cited by
22
References
26
Citations by year
2024: 1 · 2023: 1 · 2021: 1 · 2014: 1
FWCI
3.46
Type
journal article
Language
en
Open access
closed
Locations
  • Experimental Lung Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL 28330)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Neonatal Respiratory Health Research
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Surgery › Transplantation: Methods and Outcomes
Keywords
fetal lung, airway branching, organ culture, alveolar type II cells, lamellar bodies, thyroxine, dexamethasone, somatostatin, transferrin, growth factors, culture conditions
MeSH terms
Animals, Cell Differentiation, Inclusion Bodies, Culture Media, Dexamethasone, Epithelial Cells, Epithelium, Fetus, Fibronectins, Growth Substances, Hormones, Immersion, Insulin, Lung, Oligopeptides, Organ Culture Techniques, Selenium, Somatostatin, Thyroxine, Transferrin, Mice

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