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Cited by 45Traumatic Brain Injury ResearchTraumatic Brain Injury and Neurovascular DisturbancesSleep and related disorders

Diffuse brain injury does not affect chronic sleep patterns in the mouse

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Rowe, Rachel K., Harrison, Jordan L., O’Hara, Bruce F., Lifshitz, Jonathan·2014·Brain Injury
DOI 10.3109/02699052.2014.888768PMC PMC7482552
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Abstract

PRIMARY OBJECTIVE: To test if the current model of diffuse brain injury produces chronic sleep disturbances similar to those reported by TBI patients. METHODS AND PROCEDURES: Adult male C57BL/6 mice were subjected to moderate midline fluid percussion injury (n = 7; 1.4 atm; 6-10 minutes righting reflex time) or sham injury (n = 5). Sleep-wake activity was measured post-injury using a non-invasive, piezoelectric cage system. Chronic sleep patterns were analysed weekly for increases or decreases in percentage sleep (hypersomnia or insomnia) and changes in bout length (fragmentation). MAIN OUTCOMES AND RESULTS: During the first week after diffuse TBI, brain-injured mice exhibited increased mean percentage sleep and mean bout length compared to sham-injured mice. Further analysis indicated the increase in mean percentage sleep occurred during the dark cycle. Injury-induced changes in sleep, however, did not extend beyond the first week post-injury and were not present in weeks 2-5 post-injury. CONCLUSIONS: Previously, it has been shown that the midline fluid percussion model used in this study immediately increased post-traumatic sleep. The current study extended the timeline of investigation to show that sleep disturbances extended into the first week post-injury, but did not develop into chronic sleep disturbances. However, the clinical prevalence of TBI-related sleep-wake disturbances warrants further experimental investigation.

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  • Some studies show no significant differences in orexin neurons between injured and sham-injured animals.

    “Some studies show no significant differences in orexin neurons between injured and sham-injured animals.44,67,69,71,74”

    From Effects of Traumatic Brain Injury on the Orexin/Hypocretin System (2025) · cites this paper as [69]

    The Methods and Results (page not available in our copy) compare sleep patterns in injured and sham-injured mice, not orexin neurons. The Discussion mentions the orexin signaling system as a possible contributor to post-injury sleepiness, but reports no comparison of orexin neurons between the groups (searched: orexin neurons, hypocretin, cell counts). (LLM, conf=0.76)

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Authors
Rowe, R. K., Harrison, J. L., O’Hara, B. F., & Lifshitz, J.
Affiliations
  • Barrow Neurological Institute (US)
  • University of Kentucky (US)
Corresponding author
Lifshitz, Jonathan
ORCID
Venue
Brain Injury
Publisher
Informa UK Limited
Published
2014-04-01
Volume
28
Issue
4
Pages
504-510
ISSN
  • 0269-9052 (ISSN-L)
  • 1362-301X
DOI
10.3109/02699052.2014.888768
PMC
PMC7482552
OpenAlex
W2088655509
Cited by
45
References
3
Citations by year
2026: 3 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 6 · 2017: 2 · 2016: 5 · 2015: 5 · 2014: 1 · 2013: 2
FWCI
3.28
Type
journal article
Language
en
Open access
Locations
  • Brain Injury (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Neurological Disorders and Stroke (R01NS065052, R01 NS065052-S, R21NS072611)
Topics
  • Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
  • Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and related disorders
Keywords
traumatic brain injury, sleep-wake disturbances, fluid percussion injury, hypersomnia, insomnia, sleep fragmentation, mouse model
MeSH terms
Animals, Brain Injuries, Male, Mice, Inbred C57BL, Movement, Sleep Wake Disorders, Time Factors, Wakefulness, Mice, Interleukin-1beta

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