The DOI matches its Crossref record; 213 of the 214 references it cites were found in a registry or an index.
Whole-cell biochips for bio-sensing: integration of live cells and inanimate surfaces
Abstract
Recent advances in the convergence of the biological, chemical, physical, and engineering sciences have opened new avenues of research into the interfacing of diverse biological moieties with inanimate platforms. A main aspect of this field, the integration of live cells with micro-machined platforms for high throughput and bio-sensing applications, is the subject of the present review. These unique hybrid systems are configured in a manner that ensures positioning of the cells in designated patterns, and enables cellular viability maintenance, and monitoring of cellular functionality. Here we review both animate and inanimate surface properties and how they affect cellular attachment, describe relevant modifications of both types of surfaces, list technologies for platform engineering and for cell deposition in the desired configurations, and discuss the influence of various deposition and immobilization methods on the viability and performance of the immobilized cells.
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“Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer. 83,84”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
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“Silicon offers advantageous electrical and mechanical properties but lacks optical transparency, thus limiting the utility of luminescent and fluorescent analytical techniques. 83”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
In the citing paper
“Plastics, although inexpensive, are autofluorescent and have a tendency to take up water and other solvents-attributes that can skew fluorescence signals from the sensing element. 83”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
Citations
Cites
Hynes (2002) · doi:10.1016/s0092-8674(02)00971-6 · Cited by 8,606
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382
Iler, K. (1979) · Medical Entomology and Zoology · Cited by 5,030
Geometric Control of Cell Life and Death
Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., & Ingber, D. E. (1997) · Science · doi:10.1126/science.276.5317.1425 · Cited by 4,710
Younan Xia, A., & Whitesides, G. M. (1998) · Annual Review of Materials Science · doi:10.1146/annurev.matsci.28.1.153 · Cited by 4,610
Silicon as a mechanical material
Petersen, K. (1982) · Proceedings of the IEEE · doi:10.1109/proc.1982.12331 · Cited by 2,925
Jones, T. B. (1995) · doi:10.1017/cbo9780511574498 · Cited by 2,786
A review on fundamentals and applications of electrophoretic deposition (EPD)
Besra, L., & Liu, M. (2006) · Progress in Materials Science · doi:10.1016/j.pmatsci.2006.07.001 · Cited by 2,310
Transmembrane crosstalk between the extracellular matrix and the cytoskeleton
Geiger, B., Bershadsky, A. D., Pankov, R. G., & Yamada, K. M. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35099066 · Cited by 2,213
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,105
Research works printed but not linked: 11
- Brandenburg A, 2009, Adv Biomed Clin Diagn Syst VII
- Colpo P, 2010, Adv Biochem Engin/Biotechnol, 117, 109
- Date A, 2010, Adv Biochem Eng / Biotechnol, 117, 57
- Kintzios SE., 2006, Front Drug Des Discov, 2, 225
- Melamed S, 2010, Lab on a Chip
- Muller R, 2007, J Biomed Mater Res A, 84, 817
- Parviz M, 2007, Neuro Toxicology, 28, 520
- Shimomura-Shimizu M, 2010, Adv Biochem Eng/Biotechnol, 118, 1
- Sze SM. (1988). VLSI technology. New York, McGraw-Hill.
- Van der Meer JR, 2010, Annu Rev Microbiol, 8
- Yasuda H., 1981, J Polym Sci: Macromol Rev, 16, 199
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Metadata
- Authors
- Ben-Yoav, H., Melamed, S., Freeman, A., Shacham-Diamand, Y., & Belkin, S.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Tel Aviv University (IL)
- Venue
- Critical Reviews in Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2010-12-30
- Volume
- 31
- Issue
- 4
- Pages
- 337-353
- ISSN
- 0738-8551 (ISSN-L)
- 1549-7801
- OpenAlex
- W1969803714
- Cited by
- 52
- References
- 214
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 6 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 7 · 2012: 7
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Critical Reviews in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- biosensing, cell patterning, cell-surface interactions, surface modification, cell viability
- MeSH terms
- Bacteria, Cell Adhesion, Cell Survival, Surface Properties, Biosensing Techniques, Cells, Immobilized, Microfluidic Analytical Techniques, Microarray Analysis
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