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A Simple Methylene Blue-Azure Ii-Basic Fuchsin Stain for Epoxy-Embedded Tissue Sections
Abstract
One micron-thick sections of tissues fixed in glutaraldehyde, or in glutaraldehyde followed by osmium tetroxide, and embedded in a variety of plastic resins were stained in a methylene blue-azure II solution at 65 C, then counterstained in 0.05% basic fuchsin in 2.5% ethanol at room temperature (24 C). Considerable variation was found in methylene blue-azure II staining times for different embedding media. Aged Epon-812 required less staining time than freshly polymerized blocks of Epon-812. The procedure is a simple, rapid staining technique suitable for photomicrography and tissue orientation for electron microscopy.
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Cited by
Drummond, I. A., Majumdar, A., Hentschel, H., Elger, M., Solnica-Krezel, L., Schier, A. F., Neuhauss, S. C. F., Stemple, D. L., Zwartkruis, F., Rangini, Z., et al. (1998) · Development · doi:10.1242/dev.125.23.4655 · Cited by 486
Unique and conserved aspects of gut development in zebrafish
Wallace, K. N., & Pack, M. (2003) · Developmental Biology · doi:10.1016/s0012-1606(02)00034-9 · Cited by 349
Halpern, M. E., Thisse, C., Ho, R. K., Thisse, B., Riggleman, B., Trevarrow, B., Weinberg, E. S., Postlethwait, J. H., & Kimmel, C. B. (1995) · Development · doi:10.1242/dev.121.12.4257 · Cited by 178
Characterization of endosteal bone‐lining cells from fatty marrow bone sites in adult beagles
Miller, S. C., Bowman, B. M., Smith, J. M., & Jee, W. S. S. (1980) · The Anatomical Record · doi:10.1002/ar.1091980204 · Cited by 98
Metadata
- Authors
- Humphrey, C. D., & Pittman, F. E.
- Venue
- Stain Technology
- Publisher
- Informa UK Limited
- Published
- 1974-01
- Volume
- 49
- Issue
- 1
- Pages
- 9-14
- ISSN
- 0038-9153 (ISSN-L)
- OpenAlex
- W2221233133
- Cited by
- 505
- References
- 0
- FWCI
- 5.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Humphrey, C. D., & Pittman, F. E. (1974). A Simple Methylene Blue-Azure Ii-Basic Fuchsin Stain for Epoxy-Embedded Tissue Sections. Stain Technology. https://doi.org/10.3109/10520297409116929
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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