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A New Method Using Hexamethyldisilazane for Preparation of Soft Insect Tissues for Scanning Electron Microscopy
Abstract
A new rapid procedure for preparing soft internal tissues from insects that allows air drying was found to compare favorably with tissues prepared by critical point drying. In the new procedure, tissues were fixed in 1% glutaraldehyde, dehydrated through a graded ethanol series, immersed in hexamethyldisilazane (HMDS) for 5 minutes, and air dried. Tissues prepared by both the HMDS treatment and by critical point drying were coated with gold for scanning electron microscopy. Tissues prepared by the HMDS treatment did not shrink or distort upon air drying and excellent surface detail was preserved. The HMDS treatment required about 5 minutes, whereas the critical point drying procedure required about 1.5 hours.
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Cites
ANDERSON, T. F. (1951) · Transactions of the New York Academy of Sciences · doi:10.1111/j.2164-0947.1951.tb01007.x · Cited by 1,482
Preparation of animal tissues for surface‐scanning electron microscopy
Boyde, A., & Wood, C. (1969) · Journal of Microscopy · doi:10.1111/j.1365-2818.1969.tb00709.x · Cited by 345
Cited by
Gates, R. D., Baghdasarian, G., & Muscatine, L. (1992) · The Biological Bulletin · doi:10.2307/1542252 · Cited by 516
Mineral stimulation of subsurface microorganisms: release of limiting nutrients from silicates
Rogers, J. R., & Bennett, P. C. (2004) · Chemical Geology · doi:10.1016/j.chemgeo.2003.09.001 · Cited by 296
Bray, D. F., Bagu, J., & Koegler, P. (1993) · Microscopy Research and Technique · doi:10.1002/jemt.1070260603 · Cited by 285
Fossilization of Soft Tissue in the Laboratory
Briggs, D. E. G., & Kear, A. J. (1993) · Science · doi:10.1126/science.259.5100.1439 · Cited by 268
Decay and mineralization of shrimps
Briggs, D. E. G., & Kear, A. J. (1994) · PALAIOS · doi:10.2307/3515135 · Cited by 260
Metadata
- Authors
- Nation, J. L.
- Venue
- Stain Technology
- Publisher
- Informa UK Limited
- Published
- 1983-01
- Volume
- 58
- Issue
- 6
- Pages
- 347-351
- ISSN
- 0038-9153 (ISSN-L)
- OpenAlex
- W2001512934
- Cited by
- 771
- References
- 2
- FWCI
- 3.70
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nation, J. L. (1983). A New Method Using Hexamethyldisilazane for Preparation of Soft Insect Tissues for Scanning Electron Microscopy. Stain Technology. https://doi.org/10.3109/10520298309066811
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
