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Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol
Abstract
Polyethylene glycol (400) with 90% glycerol (aqueous) is introduced as an efficient solvent system for lipid stains. Various lipid-soluble dyes were dissolved in this solvent system and tested for their intensity, contrast, and specificity of staining of suberin lamellae in plant tissue. The stability (i.e., lack of precipitation) of the various staining solutions in the presence of fresh tissue was also tested. When dissolved in polyethylene glycol-glycerol, Sudan red 7B (fat red) was the best nonfluorescent stain and fluorol yellow 088 (solvent green 4) was an excellent fluorochrome. These two dyes formed stable staining solutions which efficiently stained lipids in fresh sections without forming precipitates. Estimations of the solubilities of these dyes in the solvent compared with their solubilities in lipids of various chemical types indicated that they should both be effective stains for lipids in general.
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Cited by
Root responses to cadmium in the rhizosphere: a review
Lux, A., Martinka, M., Vaculik, M., & White, P. J. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/erq281 · Cited by 1,100
Beisson, F., Li, Y., Bonaventure, G., Pollard, M., & Ohlrogge, J. B. (2007) · The Plant Cell · doi:10.1105/tpc.106.048033 · Cited by 448
Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M. X., Arondel, V., Bates, P. D., Baud, S., Bird, D., DeBono, A., Durrett, T. P., et al. (2010) · The Arabidopsis Book · doi:10.1199/tab.0133 · Cited by 367
ABCG Transporters Are Required for Suberin and Pollen Wall Extracellular Barriers in Arabidopsis
Yadav, V., Molina, I., Ranathunge, K., Castillo, I. Q., Rothstein, S. J., & Reed, J. W. (2014) · The Plant Cell · doi:10.1105/tpc.114.129049 · Cited by 306
Höfer, R., Briesen, I., Beck, M., Pinot, F., Schreiber, L., & Franke, R. (2008) · Journal of Experimental Botany · doi:10.1093/jxb/ern101 · Cited by 304
Metadata
- Authors
- Brundrett, M. C., Kendrick, B., & Peterson, C. A.
- Venue
- Biotechnic & Histochemistry
- Publisher
- Informa UK Limited
- Published
- 1991-01
- Volume
- 66
- Issue
- 3
- Pages
- 111-116
- ISSN
- 1052-0295 (ISSN-L)
- 1473-7760
- OpenAlex
- W2089904284
- Cited by
- 600
- References
- 0
- FWCI
- 3.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol. https://app.cochat.ai/papers/10.3109/10520299109110562
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- Brundrett, M. C., Kendrick, B., & Peterson, C. A. (1991). Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol. Biotechnic & Histochemistry. https://doi.org/10.3109/10520299109110562
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