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Cited by 600

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Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol

Brundrett, Mark C., Kendrick, Bryce, Peterson, Carol A.·1991·Biotechnic & Histochemistry

Abstract

Polyethylene glycol (400) with 90% glycerol (aqueous) is introduced as an efficient solvent system for lipid stains. Various lipid-soluble dyes were dissolved in this solvent system and tested for their intensity, contrast, and specificity of staining of suberin lamellae in plant tissue. The stability (i.e., lack of precipitation) of the various staining solutions in the presence of fresh tissue was also tested. When dissolved in polyethylene glycol-glycerol, Sudan red 7B (fat red) was the best nonfluorescent stain and fluorol yellow 088 (solvent green 4) was an excellent fluorochrome. These two dyes formed stable staining solutions which efficiently stained lipids in fresh sections without forming precipitates. Estimations of the solubilities of these dyes in the solvent compared with their solubilities in lipids of various chemical types indicated that they should both be effective stains for lipids in general.

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Metadata

Authors
Brundrett, M. C., Kendrick, B., & Peterson, C. A.
Venue
Biotechnic & Histochemistry
Published
1991-01
Volume
66
Issue
3
Pages
111-116
ISSN
  • 1052-0295 (ISSN-L)
  • 1473-7760
OpenAlex
W2089904284
Cited by
600
References
0
FWCI
3.87
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol. https://app.cochat.ai/papers/10.3109/10520299109110562
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Brundrett, M. C., Kendrick, B., & Peterson, C. A. (1991). Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol. Biotechnic & Histochemistry. https://doi.org/10.3109/10520299109110562

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.