The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis
Open accessAbstract
Suberin and cutin are fatty acid– and glycerol-based plant polymers that act as pathogen barriers and function in the control of water and solute transport. However, despite important physiological roles, their biosynthetic pathways, including the acyl transfer reactions, remain hypothetical. We report the characterization of two suberin mutants (gpat5-1 and gpat5-2) of Arabidopsis thaliana GPAT5, encoding a protein with acyl-CoA:glycerol-3-phosphate acyltransferase activity. RT-PCR and β-glucuronidase–promoter fusion analyses demonstrated GPAT5 expression in seed coat, root, hypocotyl, and anther. The gpat5 plants showed a 50% decrease in aliphatic suberin in young roots and produced seed coats with a severalfold reduction in very long chain dicarboxylic acid and ω-hydroxy fatty acids typical of suberin but no change in the composition or content of membrane or storage glycerolipids or surface waxes. Consistent with their altered suberin, seed coats of gpat5 mutants had a steep increase in permeability to tetrazolium salts compared with wild-type seed coats. Furthermore, the germination rate of gpat5 seeds under high salt was reduced, and gpat5 seedlings had lower tolerance to salt stress. These results provide evidence for a critical role of GPAT5 in polyester biogenesis in seed coats and roots and for the importance of lipid polymer structures in the normal function of these organs.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
Murashige, T., & Skoog, F. (1962) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1962.tb08052.x · Cited by 67,392
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Alonso, J. M., Stepanova, A. N., Leisse, T. J., Kim, C. J., Chen, H., Shinn, P., Stevenson, D. K., Zimmerman, J., Barajas, P., Cheuk, R., et al. (2003) · Science · doi:10.1126/science.1086391 · Cited by 5,102
Assaying chimeric genes in plants: The GUS gene fusion system
Jefferson, R. A. (1987) · Plant Molecular Biology Reporter · doi:10.1007/bf02667740 · Cited by 4,486
Seed Germination and Dormancy.
Bewley, J. D. (1997) · The Plant Cell · doi:10.1105/tpc.9.7.1055 · Cited by 3,137
A gene expression map of Arabidopsis thaliana development
Schmid, M., Davison, T. S., Henz, S. R., Pape, U. J., Demar, M., Vingron, M., Schölkopf, B., Weigel, D., & Lohmann, J. U. (2005) · Nature Genetics · doi:10.1038/ng1543 · Cited by 2,523
In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants
Bechtold, N., Baquero, M., Bonhomme, S., Ferault, M., Horlow, C., Laroche, L., Migdal, M., Mollier, P., Montoro, P., Roux, D., et al. (1993) · HAL · Cited by 1,774
A Gene Expression Map of the Arabidopsis Root
Birnbaum, K., Shasha, D. E., Wang, J. Y., Jung, J. W., Lambert, G. M., Galbraith, D. W., & Benfey, P. N. (2003) · Science · doi:10.1126/science.1090022 · Cited by 1,258
GENETICS AND BIOCHEMISTRY OF SEED FLAVONOIDS
Lepiniec, L., Debeaujon, I., Routaboul, J.-M., Baudry, A., Pourcel, L., Nesi, N., & Caboche, M. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.57.032905.105252 · Cited by 1,242
Koornneef, M., Bentsink, L., & Hilhorst, H. (2002) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(01)00219-9 · Cited by 1,180
Di Laurenzio, L., Wysocka-Diller, J., Malamy, J. E., Pysh, L., Helariutta, Y., Freshour, G., Hahn, M. G., Feldmann, K. A., & Benfey, P. N. (1996) · Cell · doi:10.1016/s0092-8674(00)80115-4 · Cited by 1,124
Cited by
Baxter, I., Hosmani, P. S., Rus, A., Lahner, B., Borevitz, J. O., Muthukumar, B., Mickelbart, M. V., Schreiber, L., Franke, R. B., & Salt, D. E. (2009) · PLoS Genetics · doi:10.1371/journal.pgen.1000492 · Cited by 342
The role of the testa during development and in establishment of dormancy of the legume seed.
Smýkal, P., Vernoud, V., Blair, M. W., Soukup, A., & Thompson, R. D. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00351 · Cited by 331
Fukuda, S., Hirasawa, E., Takemura, T., Takahashi, S., Chokshi, K., Pancha, I., Tanaka, K., & Imamura, S. (2018) · Scientific Reports · doi:10.1038/s41598-018-30809-8 · Cited by 78
Metadata
- Authors
- Beisson, F., Li, Y., Bonaventure, G., Pollard, M., & Ohlrogge, J. B.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-01-01
- Volume
- 19
- Issue
- 1
- Pages
- 351-368
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2130269975
- Cited by
- 448
- References
- 60
- FWCI
- 23.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis. https://app.cochat.ai/papers/10.1105/tpc.106.048033
- The paper
- Beisson, F., Li, Y., Bonaventure, G., Pollard, M., & Ohlrogge, J. B. (2007). The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis. The Plant Cell. https://doi.org/10.1105/tpc.106.048033
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
