The Central Effects of a Nitric Oxide Synthase Inhibitor (Nω- Nitro - L - Arginine) on Blood Pressure and Plasma Renin
Abstract
An endothelium-derived relaxing factor has been identified as nitric oxide (NO). Peripheral and central administration of nitric oxide synthase inhibitors result in an increase in renal sympathetic nerve activity and an increase in blood pressure. The goal of our study was to determine if the increase in blood pressure following central NO synthase inhibition with N omega-nitro-L-arginine (L-NNA) is caused by the release of renin. Six groups of Sprague-Dawley rats were used. Group I (control) received intracerebroventricular (i.c.v.) artificial cerebrospinal fluid. Groups II & III received i.c.v. L-NNA, 5 & 15 micrograms/min. respectively. Group IV was treated with intravenous L-NNA, 15 micrograms/min. Group V, after bilateral nephrectomy, received i.c.v. L-NNA, 15 micrograms/min. Group VI received i.c.v. L-arginine, 60 micrograms/min. and i.c.v. L-NNA, 15 micrograms/min., simultaneously. Plasma renin concentration was measured in groups I, III, IV & V. Mean arterial blood pressure was significantly increased in groups II, III & V, i.e., following i.c.v. infusion of L-NNA. The increase in mean arterial blood pressure was significantly greater when the dose was increased from 5 to 15 micrograms/min. and it was eliminated when L-arginine was added to the infusion. The increase in blood pressure was attended by no change in heart rate. While the plasma renin concentration increased significantly in group III, this could not explain the increase in blood pressure since the nephrectomized group (V) showed no increase in renin concentration but an equivalent increase in blood pressure. The results show that acute central administration of a low dose of L-NNA increases blood pressure in rats and this increase can be prevented by central administration of L-arginine. However, intravenous infusion of the same dose (15 micrograms/min.) of L-NNA does not change blood pressure. We conclude that L-NNA acts directly within the central nervous system to increase blood pressure by a renin-independent mechanism. These results imply that central nitric oxide plays an important role in the regulation of blood pressure.
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NOS1 activity in the medulla and hypothalamus is important for systemic regulation of blood pressure.
“NOS1 activity in the medulla and hypothalamus is important for systemic regulation of blood pressure [104-107]”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [106]
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Nitric oxide synthases: regulation and function
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- Authors
- El karib, A. O., Sheng, J., Betz, A. L., & Malvin, R. L.
- Affiliations
- University of Michigan (US)
- Venue
- Clinical and Experimental Hypertension
- Publisher
- Informa UK Limited
- Published
- 1993-01
- Volume
- 15
- Issue
- 5
- Pages
- 819-832
- ISSN
- 0148-3927 (ISSN-L)
- 1064-1963
- 1525-6006
- OpenAlex
- W2122696877
- Cited by
- 48
- References
- 29
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical and Experimental Hypertension (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (P01HL018575, HL 18575)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- nitric oxide synthase inhibition, L-NNA, nitric oxide, central nervous system, blood pressure regulation, plasma renin, L-arginine, renal sympathetic nerve activity, intracerebroventricular administration, bilateral nephrectomy
- MeSH terms
- Amino Acid Oxidoreductases, Animals, Arginine, Blood Pressure, Injections, Intravenous, Injections, Intraventricular, Kidney, Male, Nephrectomy, Renin, Sympathetic Nervous System, Rats, Sprague-Dawley, Nitric Oxide Synthase, Nitroarginine, Rats
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