The onset of lipid peroxidation in rheumatoid arthritis: consequences and monitoring
Abstract
Several epidemiological studies propose the association of rheumatoid arthritis (RA) with oxidative stress. The aim of this study was to estimate the possible onset of systemic lipid peroxidation in RA patients and its relevance for pathophysiology and monitoring of RA. Seventy-three patients with RA and 73 healthy subjects were included in the study. Lipid peroxidation was estimated by the measurement of 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal, malondialdehyde, acrolein, crotonaldehyde, 4-oxononenal, and isoprostanes (8-isoPGF(2α)) levels. Cytosolic phospholipase A(2) (cPLA(2)), platelet-activating factor acetylhydrolase (PAF-AH) and glutathione peroxidase (GSH-Px) activities and vitamin E levels were also determined. In parallel, the plasma levels of phospholipid arachidonic acid (AA), linoleic acid (LA), and 4-HNE-protein adducts were monitored. Plasma of RA patients had increased vitamin E levels, but decreased GSH-Px activity and phospholipid AA and LA levels when compared to levels of the healthy subjects. The levels of aldehydes were significantly increased in the plasma of the RA patients and even more in urine. Significant increases in HNE-modified protein adducts was observed for the first time in plasma of RA patients, while the activities of PAF-AH and cPLA(2) were decreased. The 8-isoPGF(2α) levels were 9-fold higher in plasma and 3-fold higher in urine of RA patients and were related to the severity of disease. The levels of lipid peroxidation products in pla…
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RA patients have higher levels of free and protein-bound 4-HNE in urine and plasma than control subjects.
“For instance, RA patients consistently revealed higher levels of free and protein-bound 4-HNE in urine and plasma compared to control subjects [177].”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [177]
Plasma and urine MDA levels are elevated in RA patients (n = 73 per group) compared with healthy subjects, as measured by GC-MS.
“| RA | Plasma, urine | Healthy subjects vs. RA patients (n = 73 per group) | GC-MS | Elevated levels of MDA in plasma and urine of RA patients | [177] |”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [177]
Plasma and urine levels of free and protein-bound 4-HNE are elevated in RA patients (n = 73 per group) compared with healthy subjects, as measured by GC-MS and ELISA.
“| RA | Plasma, urine | Healthy subjects vs. RA patients (n = 73 per group) | GC-MS, ELISA | Elevated levels of free and protein-bound 4-HNE in RA patients | [177] |”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [177]
Luczaj et al. found elevated MDA levels in different specimens of RA and tick-borne encephalitis patients compared to controls using GC-MS.
“The GC-MS approach in the study by Luczaj and colleagues as mentioned above, has been also used for the measurement of MDA, revealing elevated levels in different specimens of RA and thick-born encephalitis patients compared to controls [177,178].”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [177]
Cites
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Thiol-based redox switches in eukaryotic proteins
Brandes, N., Schmitt, S., & Jakob, U. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2285 · Cited by 373 · see citing works
Two Distinct Pathways of Formation of 4-Hydroxynonenal
Schneider, C., Tallman, K. A., Porter, N. A., & Brash, A. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m101821200 · Cited by 313 · see citing works
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Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases
Kehm, R., Baldensperger, T., Raupbach, J., & Höhn, A. (2021) · Redox Biology · doi:10.1016/j.redox.2021.101901 · Cited by 348 · see citing works
“The GC-MS approach in the study by Luczaj and colleagues as mentioned above, has been also used for the measurement of MDA, revealing elevated levels in different specimens of RA and thick-born encephalitis patients compared to controls [177,178].”
BackgroundMethodResultInfluential
- Authors
- Łuczaj, W., Gindzienska-Sieskiewicz, E., Jarocka-Karpowicz, I., Andrisic, L., Sierakowski, S., Zarkovic, N., Waeg, G., & Skrzydlewska, E.
- Affiliations
- Medical University of Białystok (PL)
- University of Graz (AT)
- Corresponding author
- Łuczaj, Wojciech
- Venue
- Free Radical Research
- Publisher
- Informa UK Limited
- Published
- 2016-01-14
- Volume
- 50
- Issue
- 3
- Pages
- 304-313
- ISSN
- 1029-2470 (ISSN-L)
- 1071-5762
- OpenAlex
- W2284471954
- Cited by
- 95
- References
- 3
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 13 · 2023: 8 · 2022: 12 · 2021: 10 · 2020: 11 · 2019: 4 · 2018: 16 · 2017: 10 · 2016: 1
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- rheumatoid arthritis, lipid peroxidation, oxidative stress, 4-hydroxynonenal, isoprostanes, malondialdehyde, aldehydes, vitamin E, glutathione peroxidase, phospholipase A2, lipoprotein-associated phospholipase A2, arachidonic acid, linoleic acid, disease severity, biomarkers, disease surveillance
- MeSH terms
- Adult, Aged, Aged, 80 and over, Aldehydes, Antioxidants, Arthritis, Rheumatoid, Fatty Acids, Unsaturated, Female, Humans, Male, Middle Aged, Lipid Peroxidation, Biomarkers, Young Adult
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