No adverse effects of clodronate on fracture healing in rats.
Open accessAbstract
Clodronate was administered daily 28 days before and after an experimental tibial fracture in 35 male rats, and the effect on fracture healing and posttraumatic bone loss was studied. 5 groups were tested. The clodronate/clodronate group received clodronate in daily doses of 10 mg/kg body weight for 28 days before being subjected to a standardized fracture of the right tibia, and during the fracture healing period of 28 days. The clodronate/saline group received clodronate before fracture and saline during the healing period. The saline/clodronate group received saline before and clodronate after fracture. The saline/saline group received saline only, while the control group served as unfractured, untreated controls. After 28 days of fracture healing, the tibias were evaluated with dual energy x-ray absorptiometry, and tested mechanically in a 3-point ventral bending test. Bone mineral content and bone mineral density were approximately 30% higher in the groups receiving clodronate during the experiment, compared to the untreated groups. The weight and cross-sectional area of the fracture callus were equal in all groups. Whether clodronate was administered before the fracture, after the fracture or both, did not affect the bone mineral. Ultimate bending moment, energy absorption, stiffness and deflection were not significantly different between the groups. Our findings suggest that clodronate increases bone mineral both when given before and after a tibial shaft fracture, without affecting fracture healing at 28 days.
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The larger calluses at week 8 are consistent with delayed callus remodeling, a phenomenon typically associated with bisphosphonates and other antiresorptive therapies.
“These findings are consistent with a delay in callus remodeling, a phenomenon that is typically associated with bisphosphonates and other antiresorptive therapies in similar models (364 -366).”
From Receptor Activator of Nuclear Factor κB Ligand and Osteoprotegerin Regulation of Bone Remodeling in Health and Disease (2008) · cites this paper as [366]
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Cites
Liberman, U. A., Weiss, S. R., Bröll, J., Minne, H. W., Quan, H., Bell, N. H., Rodriguez-Portales, J. A., Downs, R. W., Dequeker, J., Favus, M. J., et al. (1995) · New England Journal of Medicine · doi:10.1056/nejm199511303332201 · Cited by 2,276 · see citing works
Intermittent Cyclical Etidronate Treatment of Postmenopausal Osteoporosis
Watts, N. B., Harris, S. T., Genant, H. K., Wasnich, R. D., Miller, P. D., Jackson, R. D., Licata, A. A., Ross, P. D., Woodson, G. C., Yanover, M., et al. (1990) · New England Journal of Medicine · doi:10.1056/nejm199007123230201 · Cited by 947 · see citing works
Storm, T., Thamsborg, G., Steiniche, T., Genant, H. K., & Sørensen, O. H. (1990) · New England Journal of Medicine · doi:10.1056/nejm199005033221803 · Cited by 932 · see citing works
The biology of fracture healing. An overview for clinicians. Part I.
Frost, H. M. (1989) · PubMed · Cited by 600 · see citing works
Cellular Biology and Biochemical Mechanism of Bone Resorption
Vaes, G. (1988) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-198806000-00033 · Cited by 508 · see citing works
The ultimate properties of bone tissue: The effects of yielding
Burstein, A. H., Currey, J. D., Frankel, V. H., & Reilly, D. T. (1972) · Journal of Biomechanics · doi:10.1016/0021-9290(72)90017-6 · Cited by 254 · see citing works
Outcome of bone mineral density in anorexia nervosa patients 11.7 years after first admission
Herzog, W., Minne, H. W., Deter, H., Leidig, G., Schellberg, D., Wüster, C. C., Gronwald, R. G., Sarembe, E., Kröger, F., Bergmann, G., et al. (1993) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650080511 · Cited by 191 · see citing works
Intermittent Cyclical Etidronate Treatment of Postmenopausal Osteoporosis
Watts, N. B., Harris, S. T., Genant, H. K., WASNICH, E. D., Miller, P. D., Jackson, R. D., Licata, A. A., Ross, P. D., Woodson, G. C., Yanover, M., et al. (1991) · Obstetrical & Gynecological Survey · doi:10.1097/00006254-199101000-00023 · Cited by 190 · see citing works
A standard test for laboratory animal bone
Burstein, A. H., & Frankel, V. H. (1971) · Journal of Biomechanics · doi:10.1016/0021-9290(71)90026-1 · Cited by 181 · see citing works
Ekeland, A., Engesaeter, L. B., & Langeland, N. (1981) · Acta Orthopaedica Scandinavica · doi:10.3109/17453678108992155 · Cited by 110 · see citing works
36 Holding Power of Orthopedic Screws in Bone
Koranyi, E., Bowman, C., Knecht, C. D., et al. (1970) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-197009000-00037 · Cited by 109 · see citing works
Wronski, T. J., Yen, C. ‐., & Scott, K. S. (1991) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650060410 · Cited by 106 · see citing works
The long-term skeletal effects of EHDP in dogs
Flora, L., Hassing, G. S., Cloyd, G. G., et al. (1981) · Metabolic Bone Disease and Related Research · doi:10.1016/0221-8747(81)90045-x · Cited by 100 · see citing works
Effect of EHDP on fracture healing in dogs
Lenehan, T. M., Balligand, M. H., Nunamaker, D. M., & Wood, F. E. (1985) · Journal of Orthopaedic Research® · doi:10.1002/jor.1100030413 · Cited by 85 · see citing works
The neuronal regulation of fracture healing: Effects of sciatic nerve resection in rat tibia
Nordsletten, L., Madsen, J. E., Almaas, R., et al. (1994) · Acta Orthopaedica Scandinavica · doi:10.3109/17453679408995457 · Cited by 74 · see citing works
Self-citationUse of bisphosphonates in the prevention of bone loss and fractures
PARFITT, A. M. (1991) · The American Journal of Medicine · doi:10.1016/0002-9343(91)90246-t · Cited by 54 · see citing works
Nordsletten, L., Kaastad, T. S., Madsen, J. E., et al. (1994) · Calcified Tissue International · doi:10.1007/bf00298557 · Cited by 49 · see citing works
Self-citationA One Year Follow‐Up Study of the Distribution of 14C‐Clodronate in Mice and Rats
Mönkkönen, J. (1988) · Pharmacology & Toxicology · doi:10.1111/j.1600-0773.1988.tb01843.x · Cited by 48 · see citing works
Clodronate increases the calcium content in fracture callus
Nyman, M. T., Paavolainen, P., & Lindholm, T. S. (1993) · Archives of Orthopaedic and Trauma Surgery · doi:10.1007/bf00451880 · Cited by 43 · see citing works
Bisphosphonates in osteoporosis: An introduction
Fleisch, H. A. (1993) · Osteoporosis International · doi:10.1007/bf01623000 · Cited by 43 · see citing works
Journal of Bone and Mineral Research
Ott, S. M. (1993) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650081329 · Cited by 42 · see citing works
Effect of clodronate on fracture healing in denervated rats
Tarvainen, R., Olkkonen, H., Nevalainen, T., Hyvönen, P. M., Arnala, I., & Alhava, E. M. (1994) · Bone · doi:10.1016/8756-3282(94)90320-4 · Cited by 40 · see citing works
Muscle contraction increases the structural capacity of the lower leg: An in vivo study in the rat
Nordsletten, L., & Ekeland, A. (1993) · Journal of Orthopaedic Research® · doi:10.1002/jor.1100110218 · Cited by 35 · see citing works
Self-citationEpidemiology and prevention of osteoporosis in urbanized Asian populations
Lau, E. M. C., & Cooper, C. C. (1993) · Osteoporosis International · doi:10.1007/bf01621856 · Cited by 33 · see citing works
Rationale for the use of clodronate in osteoporosis
Kanis, J. А., McCloskey, E. V., Sirtori, P., Khan, S., Fern, D. L., EYRES, K. S., Aaron, J., & Beneton, M. N. C. (1993) · Osteoporosis International · doi:10.1007/bf01623223 · Cited by 28 · see citing works
Hyvönen, P. M., Karhi, T., Kosma, V., Liimola‐Luoma, L., & Hanhijärvi, H. (1994) · Pharmacology & Toxicology · doi:10.1111/j.1600-0773.1994.tb00379.x · Cited by 26 · see citing works
Lepola, V., Jalovaara, P., & Väänänen, K. (1994) · Bone · doi:10.1016/8756-3282(94)90302-6 · Cited by 17 · see citing works
Rosen, H. N., Middlebrooks, V. L., Sullivan, E., et al. (1994) · Calcified Tissue International · doi:10.1007/bf00425871 · Cited by 9 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Frost H M. The biology of fracture healing. An overview for cli- nicians. 1 and 11. Clin Orthop 1989; 248: 283-309.
- Koranyi E, Bowman E, Knecht C D, Janssen M. Holding power of orthopaedic screws in bone. Clin Orthop 1970; 72: 283-6.
- Madsen J E, Almaas R, Nordsletten L. Strength reduction of the femoral shaft and neck after ipsilateral tibia1 fracture in the rat. Eur J Exp Musculoskel Res 1995; 4: 65-8.
- Ott S M. Clinical effects of hisphosphonates in involutional os- teoporosis. J Bone Miner Res (Suppl) 1993; 8: 597-606.
Cited by
Sorted by citations
Kearns, A. E., Khosla, S., & Kostenuik, P. J. (2008) · Endocrine Reviews · doi:10.1210/er.2007-0014 · Cited by 824 · see citing works
Cao, Y., Mori, S., Mashiba, T., Westmore, M. S., Ma, L., Sato, M., Akiyama, T., Shi, L., Komatsubara, S., Miyamoto, K., et al. (2002) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2002.17.12.2237 · Cited by 248 · see citing works
Ulrich-Vinther, M., & Andreassen, T. T. (2005) · Calcified Tissue International · doi:10.1007/s00223-004-0126-9 · Cited by 46 · see citing works
- Authors
- Madsen, J. E., Berg-larsen, T., Kirkeby, O. J., Falch, J. A., & Nordsletten, L.
- Affiliations
- Oslo University Hospital (NO)
- University of Oslo (NO)
- Venue
- Acta Orthopaedica Scandinavica
- Published
- 1998-01
- Volume
- 69
- Issue
- 5
- Pages
- 532-536
- ISSN
- 0001-6470 (ISSN-L)
- OpenAlex
- W2107521881
- Cited by
- 61
- References
- 29
- Citations by year
- 2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Acta Orthopaedica Scandinavica (publishedVersion)
- Acta Orthopaedica (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
- Health Sciences › Medicine › Oncology › Bone health and treatments
- Keywords
- clodronate, bisphosphonates, fracture healing, tibial fractures, bone mineral density, bone mineral content, fracture callus, bone mechanical properties
- MeSH terms
- Animals, Biomechanical Phenomena, Bone Resorption, Clodronic Acid, Disease Models, Animal, Drug Evaluation, Preclinical, Male, Premedication, Radionuclide Imaging, Tibial Fractures, Absorptiometry, Photon, Bone Density, Fracture Healing, Rats, Wistar, Rats
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