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Cited by 101Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyAnimal health and immunology

Effects of feeding a calf starter on molecular adaptations in the ruminal epithelium and liver of Holstein dairy calves

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Laarman, A.H., Ruiz-Sanchez, A.L., Sugino, T., Guan, L.L., Oba, M.·2012·Journal of Dairy Science
DOI 10.3168/jds.2011-4788
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Abstract

The objective of this study was to elucidate the effect of feeding a calf starter on the volatile fatty acid (VFA) profile in the rumen and on expression of genes involved in epithelial intracellular pH regulation, butyrate metabolism, and hepatic urea cycle during the weaning transition. Twenty Holstein bull calves were fed either milk replacer and hay (MR) or milk replacer, hay, and a commercial texturized calf starter (MR+S) in a randomized complete block design. All calves were fed 750 g/d of milk replacer as the basal diet. Calves on the MR+S treatment were also fed starter ad libitum, and the energy intake of calves within blocks was maintained by supplementing the MR group with extra milk replacer that was equivalent to the energy intake from calf starter. Calves were killed 3 d after they consumed 680 g/d of calf starter for 3 consecutive days. Calves fed MR+S had higher VFA concentrations in the rumen (99.1±8.1 vs. 64.6±8.6 mM) and a higher molar proportion of butyrate (15.6±1.7 vs. 7.9±1.9%) than calves fed MR. Relative abundance of mRNA for monocarboxylate transporter isoform 1 was higher (1.45 vs. 0.53), and that of Na(+)/H(+) exchanger isoform 3 (0.37 vs. 0.82) and 3-hydroxy-3-methylglutaryl synthase isoform 1 (0.40 vs. 0.94) lower for the MR+S treatment compared with the MR treatment. In the liver, relative mRNA abundances of argininosuccinate synthetase isoform 1 (2.67 vs. 1.56), argininosuccinate lyase (1.44 vs. 0.99), and arginase isoform 1 (3.21 vs. 1.74) we…

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  • Proteins involved in VFA absorption and intracellular pH regulation (HMGCS, HMGCL, ATPeV1B, BDH) were expressed at a higher level in the rumen than in the cecum.

    “proteins involved in VFA absorption and intracellular pH regulation, including 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS), 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL), vacuolar H+ ATPase subunit B (ATPeV1B), and β-hydroxybutyrate dehydrogenase (BDH) [26–28], were expressed at a higher level in the rumen than in the cecum (p < 0.001, Fig. 1I).”

    From Reductive acetogenesis is a dominant process in the ruminant hindgut (2025) · cites this paper as [27]

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Authors
Laarman, A. H., Ruiz-Sanchez, A. L., Sugino, T., Guan, L. L., & Oba, M.
Affiliations
  • University of Alberta (CA)
  • Hiroshima University (JP)
Corresponding author
Oba, M.
ORCID
Venue
Journal of Dairy Science
Publisher
American Dairy Science Association
Published
2012-05
Volume
95
Issue
5
Pages
2585-2594
ISSN
0022-0302 (ISSN-L)
DOI
10.3168/jds.2011-4788
OpenAlex
W1975203172
Cited by
101
References
36
Citations by year
2026: 9 · 2025: 6 · 2024: 8 · 2023: 7 · 2022: 7 · 2021: 5 · 2020: 11 · 2019: 9 · 2018: 9 · 2017: 5 · 2016: 7 · 2015: 8 · 2014: 4 · 2013: 4 · 2012: 2
FWCI
11.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Sciences and Engineering Research Council
  • Japanese Federation of Dairy Cooperative Associations
  • Canadian Dairy Commission
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Health Sciences › Veterinary › Small Animals › Animal health and immunology
Keywords
calf starter, weaning transition, rumen volatile fatty acids, butyrate metabolism, rumen epithelium, intracellular pH regulation, MCT1, Na+/H+ exchanger 3, HMG-CoA synthase, argininosuccinate synthetase, arginase, cholesterol biosynthesis, gene expression, Holstein bull calves, milk replacer
MeSH terms
Animals, Animal Feed, Animals, Newborn, Blood Glucose, Energy Intake, Cattle, Diet, Epithelium, Fatty Acids, Volatile, Insulin, Liver, Rumen, Gene Expression, 3-Hydroxybutyric Acid, Real-Time Polymerase Chain Reaction

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