Dietary modulation of the expression of genes involved in short-chain fatty acid absorption in the rumen epithelium is related to short-chain fatty acid concentration and pH in the rumen of goats
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We have tested the hypothesis that increased concentrate intake induces mRNA abundance alterations of genes involved in short-chain fatty acid (SCFA) absorption in the rumen epithelium of goats and these changes of mRNA abundance are probably related to ruminal SCFA concentration and ruminal pH. Goats (n=12) were randomly allocated to 2 groups and fed either a low-concentrate (LC) diet (10% concentrate; n=6) or a medium-concentrate (MC) diet (35% concentrate; n=6) in 2 equal portions daily. Goats were fed separately with their respective diet for 3 wk. The goats were slaughtered 6h after the morning feeding on d 22. In vivo, goats in the MC treatment exhibited a greater ruminal SCFA concentration (81.9mM) compared with those in the LC treatment (58.0mM), and the pH decreased from 7.03 to 6.63. Correspondingly, mRNA expression of candidates for SCFA(-)/HCO3(-) exchangers, namely downregulated in adenoma (DRA), putative anion transporter 1 (PAT1), and anion exchanger 2 (AE2) were increased in the MC group. Further, upregulation in monocarboxylate transporter 1 (MCT1) and monocarboxylate transporter 4 (MCT4) mRNA abundances was observed in the MC group. The expression of genes that help the rumen epithelial cells to maintain intracellular pH, including Na(+)/H(+) exchanger 1 (NHE1), NHE2, NHE3, vacuolar H(+) ATPase subunit B (vH(+)ATPase), and Na(+)/K(+) ATPase pump subunit α1 (Na(+)/K(+)ATPase) were also enhanced in the MC group relative to the LC group. During in vitro studies…
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Proteins involved in VFA absorption and intracellular pH regulation (HMGCS, HMGCL, ATPeV1B, BDH) were expressed at a higher level in the rumen than in the cecum.
“proteins involved in VFA absorption and intracellular pH regulation, including 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS), 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL), vacuolar H+ ATPase subunit B (ATPeV1B), and β-hydroxybutyrate dehydrogenase (BDH) [26–28], were expressed at a higher level in the rumen than in the cecum (p < 0.001, Fig. 1I).”
From Reductive acetogenesis is a dominant process in the ruminant hindgut (2025) · cites this paper as [26]
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- Authors
- Yan, L., Zhang, B., & Shen, Z.
- Affiliations
- Nanjing Agricultural University (CN)
- Corresponding author
- Shen, Zanming
- ORCID
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2014-09
- Volume
- 97
- Issue
- 9
- Pages
- 5668-5675
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2082371548
- Cited by
- 77
- References
- 27
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 6 · 2017: 10 · 2016: 8 · 2015: 1
- FWCI
- 12.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Key Basic Research Program (2011CB100801)
- National Natural Science Foundation of China (30771568)
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- short-chain fatty acids, rumen epithelium, goats, concentrate intake, rumen pH, gene expression, monocarboxylate transporters, anion exchanger, intracellular pH regulation, rumen epithelial cells, rumen fermentation, rumen acidosis
- MeSH terms
- Animals, Cells, Cultured, Diet, Epithelial Cells, Epithelium, Fatty Acids, Volatile, Fermentation, Gene Expression Regulation, Goats, Hydrogen-Ion Concentration, RNA, Messenger, Rumen, Chloride-Bicarbonate Antiporters
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