Cited by 77Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyGut microbiota and health

Dietary modulation of the expression of genes involved in short-chain fatty acid absorption in the rumen epithelium is related to short-chain fatty acid concentration and pH in the rumen of goats

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Yan, Lei, Zhang, Bei, Shen, Zanming·2014·Journal of Dairy Science

Abstract

We have tested the hypothesis that increased concentrate intake induces mRNA abundance alterations of genes involved in short-chain fatty acid (SCFA) absorption in the rumen epithelium of goats and these changes of mRNA abundance are probably related to ruminal SCFA concentration and ruminal pH. Goats (n=12) were randomly allocated to 2 groups and fed either a low-concentrate (LC) diet (10% concentrate; n=6) or a medium-concentrate (MC) diet (35% concentrate; n=6) in 2 equal portions daily. Goats were fed separately with their respective diet for 3 wk. The goats were slaughtered 6h after the morning feeding on d 22. In vivo, goats in the MC treatment exhibited a greater ruminal SCFA concentration (81.9mM) compared with those in the LC treatment (58.0mM), and the pH decreased from 7.03 to 6.63. Correspondingly, mRNA expression of candidates for SCFA(-)/HCO3(-) exchangers, namely downregulated in adenoma (DRA), putative anion transporter 1 (PAT1), and anion exchanger 2 (AE2) were increased in the MC group. Further, upregulation in monocarboxylate transporter 1 (MCT1) and monocarboxylate transporter 4 (MCT4) mRNA abundances was observed in the MC group. The expression of genes that help the rumen epithelial cells to maintain intracellular pH, including Na(+)/H(+) exchanger 1 (NHE1), NHE2, NHE3, vacuolar H(+) ATPase subunit B (vH(+)ATPase), and Na(+)/K(+) ATPase pump subunit α1 (Na(+)/K(+)ATPase) were also enhanced in the MC group relative to the LC group. During in vitro studies…

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Crossref · 10.3168/jds.2013-7807

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  • Proteins involved in VFA absorption and intracellular pH regulation (HMGCS, HMGCL, ATPeV1B, BDH) were expressed at a higher level in the rumen than in the cecum.

    “proteins involved in VFA absorption and intracellular pH regulation, including 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS), 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL), vacuolar H+ ATPase subunit B (ATPeV1B), and β-hydroxybutyrate dehydrogenase (BDH) [26–28], were expressed at a higher level in the rumen than in the cecum (p < 0.001, Fig. 1I).”

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Authors
Yan, L., Zhang, B., & Shen, Z.
Affiliations
Nanjing Agricultural University (CN)
Corresponding author
Shen, Zanming
Venue
Journal of Dairy Science
Published
2014-09
Volume
97
Issue
9
Pages
5668-5675
ISSN
0022-0302 (ISSN-L)
OpenAlex
W2082371548
Cited by
77
References
27
Citations by year
2026: 5 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 6 · 2017: 10 · 2016: 8 · 2015: 1
FWCI
12.62
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Key Basic Research Program (2011CB100801)
  • National Natural Science Foundation of China (30771568)
  • Priority Academic Program Development of Jiangsu Higher Education Institutions
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
short-chain fatty acids, rumen epithelium, goats, concentrate intake, rumen pH, gene expression, monocarboxylate transporters, anion exchanger, intracellular pH regulation, rumen epithelial cells, rumen fermentation, rumen acidosis
MeSH terms
Animals, Cells, Cultured, Diet, Epithelial Cells, Epithelium, Fatty Acids, Volatile, Fermentation, Gene Expression Regulation, Goats, Hydrogen-Ion Concentration, RNA, Messenger, Rumen, Chloride-Bicarbonate Antiporters

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