Adhesions of extracellular surface-layer associated proteins in Lactobacillus M5-L and Q8-L
Open accessAbstract
Surface-layer associated proteins (SLAP) that envelop Lactobacillus paracasei ssp. paracasei M5-L and Lactobacillus casei Q8-L cell surfaces are involved in the adherence of these strain to the human intestinal cell line HT-29. To further elucidate some of the properties of these proteins, we assessed the yields and expressions of SLAP under different incubation conditions. An efficient and selective extraction of SLAP was obtained when cells of Lactobacillus were treated with 5 M LiCl at 37°C in aerobic conditions. The SLAP of Lactobacillus M5-L and Q8-L in cell extracts were visualized by SDS-PAGE and identified by Western blotting with sulfo-N-hydroxysuccinimide-biotin-labeled HT-29 cells as adhesion proteins. Atomic force microscopy contact imaging revealed that Lactobacillus strains M5-L and Q8-L normally display a smooth, homogeneous surface, whereas the surfaces of M5-L and Q8-L treated with 5 M LiCl were rough and more heterogeneous. Analysis of adhesion forces revealed that the initial adhesion forces of 1.41 and 1.28 nN obtained for normal Lactobacillus M5-L and Q8-L strains, respectively, decreased to 0.70 and 0.48 nN, respectively, following 5 M LiCl treatment. Finally, the dominant 45-kDa protein bands of Lactobacillus Q8-L and Lactobacillus M5-L were identified as elongation factor Tu and surface antigen, respectively, by liquid chromatography-tandem mass spectrometry.
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EF-Tu and GAPDH have been reported to be involved in mediating bacterial adhesion.
“Among them, EF-Tu and GAPDH were reported to be involved in mediating bacterial adhesion (Zhang et al., 2016; Wang et al., 2018)”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Zhang et al., 2016
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“Among them, EF-Tu and GAPDH were reported to be involved in mediating bacterial adhesion (Zhang et al., 2016; Wang et al., 2018), but their mediation of the interaction between bacteria and shrimp intestinal protein has not been studied.”
Background
- Authors
- Zhang, Y., Xiang, X., Lu, Q., Zhang, L., Ma, F., & Wang, L.
- Affiliations
- Harbin Institute of Technology (CN)
- Corresponding author
- Zhang, Lanwei
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2016-02
- Volume
- 99
- Issue
- 2
- Pages
- 1011-1018
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2218437816
- Cited by
- 21
- References
- 30
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2022: 2 · 2021: 4 · 2020: 1 · 2018: 6 · 2017: 2
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31301515)
- China Postdoctoral Science Foundation (2012M510093, 2013T60382)
- Natural Science Foundation of Heilongjiang Province (C201433)
- Fundamental Research Funds for the Central Universities (HIT.NSRIF.2012082)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus casei, bacterial adhesion, HT-29 cells, intestinal epithelial cells, adhesion force, atomic force microscopy, elongation factor Tu, surface antigens, probiotic bacteria, cell surface
- MeSH terms
- Animals, Bacterial Adhesion, Bacterial Proteins, Biotin, Electrophoresis, Polyacrylamide Gel, Humans, Intestines, Lactobacillus, Membrane Glycoproteins, Succinimides, Blotting, Western, Gene Expression Regulation, Bacterial, HT29 Cells
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