Cited by 283Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyAnimal Nutrition and Health

The DOI matches its Crossref record; 359 of the 359 references it cites were found in a registry or an index.

Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques

Open access

Hristov, A.N., Bannink, A., Crompton, L.A., Huhtanen, P., Kreuzer, M., McGee, M., Nozière, P., Reynolds, C.K., Bayat, A.R., Yáñez-Ruiz, D.R., Dijkstra, J., Kebreab, E., Schwarm, A., Shingfield, K.J., Yu, Z.·2019·Journal of Dairy Science

DOI 10.3168/jds.2018-15829

Open this paper in CoChat — free account

Abstract

Nitrogen is a component of essential nutrients critical for the productivity of ruminants. If excreted in excess, N is also an important environmental pollutant contributing to acid deposition, eutrophication, human respiratory problems, and climate change. The complex microbial metabolic activity in the rumen and the effect on subsequent processes in the intestines and body tissues make the study of N metabolism in ruminants challenging compared with nonruminants. Therefore, using accurate and precise measurement techniques is imperative for obtaining reliable experimental results on N utilization by ruminants and evaluating the environmental impacts of N emission mitigation techniques. Changeover design experiments are as suitable as continuous ones for studying protein metabolism in ruminant animals, except when changes in body weight or carryover effects due to treatment are expected. Adaptation following a dietary change should be allowed for at least 2 (preferably 3) wk, and extended adaptation periods may be required if body pools can temporarily supply the nutrients studied. Dietary protein degradability in the rumen and intestines are feed characteristics determining the primary AA available to the host animal. They can be estimated using in situ, in vitro, or in vivo techniques with each having inherent advantages and disadvantages. Accurate, precise, and inexpensive laboratory assays for feed protein availability are still needed. Techniques used for direct determination of rumen microbial protein synthesis are laborious and expensive, and data variability can be unacceptably large; indirect approaches have not shown the level of accuracy required for widespread adoption. Techniques for studying postruminal digestion and absorption of nitrogenous compounds, urea recycling, and mammary AA metabolism are also laborious, expensive (especially the methods that use isotopes), and results can be variable, especially the methods based on measurements of digesta or blood flow. Volatile loss of N from feces and particularly urine can be substantial during collection, processing, and analysis of excreta, compromising the accuracy of measurements of total-tract N digestion and body N balance. In studying ruminant N metabolism, nutritionists should consider the longer term fate of manure N as well. Various techniques used to determine the effects of animal nutrition on total N, ammonia- or nitrous oxide-emitting potentials, as well as plant fertilizer value, of manure are available. Overall, methods to study ruminant N metabolism have been developed over 150 yr of animal nutrition research, but many of them are laborious and impractical for application on a large number of animals. The increasing environmental concerns associated with livestock production systems necessitate more accurate and reliable methods to determine manure N emissions in the context of feed composition and ruminant N metabolism.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.3168/jds.2018-15829

Cites

359 of 359 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Nutritional ecology of the ruminant
Soest, P. J. V. (1995) · Choice Reviews Online · doi:10.5860/choice.32-4505 · Cited by 7,714
The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage
Ørskov, E. R., & McDonald, I. (1979) · The Journal of Agricultural Science · doi:10.1017/s0021859600063048 · Cited by 4,794
A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability
Sniffen, C. J., O'Connor, J. D., Soest, P. J. V., Fox, D. G., & Russell, J. B. (1992) · Journal of Animal Science · doi:10.2527/1992.70113562x · Cited by 3,506
Nitrogen losses from the soil/plant system: a review
Cameron, K. C., Di, H. J., & Moir, J. L. (2013) · Annals of Applied Biology · doi:10.1111/aab.12014 · Cited by 1,425
The Continuous Culture of Bacteria; a Theoretical and Experimental Study
Herbert, D. E., Elsworth, R., & Telling, R. C. (1956) · Journal of General Microbiology · doi:10.1099/00221287-14-3-601 · Cited by 1,024
Digestion and metabolism in the ruminant
Ziołecki, A. (1976) · Livestock Production Science · doi:10.1016/0301-6226(76)90025-7 · Cited by 998
Microbial Protein Synthesis and Flows of Nitrogen Fractions to the Duodenum of Dairy Cows
Clark, J. H., Klusmeyer, T. H., & Cameron, M. R. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77992-2 · Cited by 831
A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS
Zinn, R. A., & Owens, F. N. (1986) · Canadian Journal of Animal Science · doi:10.4141/cjas86-017 · Cited by 821
Rumen Microbial Population Dynamics during Adaptation to a High-Grain Diet
Fernando, S. C., Purvis, H. T., Najar, F. Z., Sukharnikov, L. O., Krehbiel, C. R., Nagaraja, T. G., Roe, B. A., & DeSilva, U. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00388-10 · Cited by 797
Factors affecting odd- and branched-chain fatty acids in milk: A review
Vlaeminck, B., Fievez, V., Cabrita, A. R. J., Fonseca, A. J. M., & Dewhurst, R. J. (2006) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2006.06.017 · Cited by 750

Flagged first, then by citations

Show all 359 references

Research works printed but not linked: 19

1992, Nutritive requirements of ruminant animals: Protein. Technical Committee on Responses to Nutrients, Report No. 9. Nutrition Abstracts and Reviews, Series B, 62, 787
Brandt, M., K. Rohr, and P. Lebzien. 1980. Determination of microbial protein synthesized in the rumen by means of 15N. Pages 496–503 in Protein Metabolism and Nutrition. H. J. Oslage and K. Rohr, ed. EAAP Publication No. 23, Rome, Italy.
Broderick, G. A. 1982. Estimation of protein degradation using in situ and in vitro methods. Pages 72–80 in Proceedings of an International Symposium on Protein Requirements for Cattle. F. N. Owens, ed. Oklahoma State University, Stillwater, OK.
Calsamiglia, 1994, Effect of various incubation times on in situ estimation of ruminal crude protein degradation., J. Anim. Sci., 72, 171
Grant, 2015, Time required for adaptation of behavior, feed intake, and dietary digestibility in cattle., J. Dairy Sci., 98, 312
Hristov, 2001, In vitro rates of bacterial incorporation of nitrogen fractions from 15N-labeled whole-crop barley ensiled at two dry matter contents., J. Anim. Sci., 79, 360
Hvelplund, T., J. Andrieu, M. R. Weisbjerg, and M. Vermorel. 1995. Prediction of the energy and protein value of forages for ruminants. Pages 205–227 in Recent developments in the nutrition of herbivores. M. Journet, E. Grenet, M-H. Farce, M. Theriez, and C. Demarquilly, ed. INRA Editions, Paris, France.
Hvelplund, 1994, True intestinal digestibility of protein estimated with sheep on intragastric infusion and with the mobile bag technique., Proc. Soc. Nutr. Phys., 3, 64
Hvelplund, 1992, Estimation of the true digestibility of rumen undegraded protein in the small intestine of ruminants by the mobile bag technique., Acta Agric. Scand. A. Anim. Sci., 42, 34
Lapierre, H., D. R. Ouellet, L. Doepel, G. Holtrop, and G. E. Lobley. 2008. Histidine, lysine and methionine: From metabolism to balanced dairy rations. Pages 19–36 in Proc. 44th Eastern Nutrition Conference of the Animal Nutrition Association of Canada (ANAC), University of Guelph, Guelph, ON, Canada. University of Guelph, Guelph, ON, Canada.
Madsen, 1985, The basis for the proposed Nordic protein evaluation system for ruminants. The AAT-PBV system., Acta Agric. Scand., 25, 9
Mosier, A. R. 1989. Chamber and isotope techniques. Pages 175–187 in Exchange of Trace Gases between Terrestrial Ecosystems and the Atmosphere. M. O. Andreae and D. S. Schimel, ed. John Wiley & Sons, New York, NY.
Poncet, C., B. Michalet-Doreau, T. McAllister, and D. Remond. 1995. Dietary compounds escaping rumen digestion. Pages 167–204 in Recent Developments in the Nutrition of Herbivores. M. Journet, E. Grenet, M.-H. Farce, M. Theriez, and C. Demarquilly, ed. INRA Editions, Paris, France.
Rajczyk, 1995, Postural effects on mammary blood flow and nutrient uptake., Proc. Nutr. Soc. Aust., 19, 119
Reynolds, 2001, Effects of intake and lactation on absorption and metabolism of leucine and phenylalanine by splanchnic tissues of dairy cows., J. Dairy Sci., 84, 362
Reynolds, C. K., D. J. Humphries, S. B. Cammell, J. Benson, J. D. Sutton, and D. E. Beever. 1998. Effects of abomasal wheat starch infusion on splanchnic metabolism and energy balance of lactating dairy cows. Page 39 in Energy Metabolism of Farm Animals. K. J. McCracken, E. F. Unsworth, and A. R. G. Wylie, ed. Proc. the 14th Symposium on Energy Metabolism. CAB International, Wallingford, UK.
Ross, D. A., M. Gutierrez-Botero, and M. E. Van Amburgh. 2013. Development of an in vitro intestinal digestibility assay for ruminant feeds. Pages 190–202 in Proc. Cornell Nutrition Conference, Cornell University, Ithaca, NY.
Steingaß, 2013, Proteinbewertung beim Wiederkäuer – Grundlagen, analytische Entwicklungen und Perspektiven., Übers. Tierernähr., 41, 51
Vérité, R., and J. L. Peyraud. 1989. Protein: The PDI system. Pages 33–48 in Ruminant Nutrition. R. Jarrige, ed. Recommended Allowances and Feed Tables. INRA, France.

Sorted by citations

A comprehensive assessment of the method for producing biochar, its characterization, stability, and potential applications in regenerative economic sustainability – A review
Amalina, F., Razak, A. S. A., Krishnan, S., Zularisam, A. W., & Nasrullah, M. (2022) · Cleaner Materials · doi:10.1016/j.clema.2022.100045 · Cited by 255
Improving nitrogen use efficiency by manipulating nitrate remobilization in plants
Chen, K., Chen, H., Tseng, C.-S., & Tsay, Y. (2020) · Nature Plants · doi:10.1038/s41477-020-00758-0 · Cited by 197
Urea transport and hydrolysis in the rumen: A review
Hailemariam, S., Zhao, S., He, Y., & Wang, J. (2021) · Animal nutrition · doi:10.1016/j.aninu.2021.07.002 · Cited by 100
Invited review: Advances in nutrition and feed additives to mitigate enteric methane emissions
Hristov, A. N. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24440 · Cited by 95
Self-citation
Agricultural Waste: Challenges and Solutions, a Review
Lackner, M. K., & Besharati, M. (2025) · Waste · doi:10.3390/waste3020018 · Cited by 116
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review
Liang, J., Zhang, R., Chang, J., Chen, L., Nabi, M., Zhang, H., Zhang, G., & Zhang, P. (2024) · Biotechnology Advances · doi:10.1016/j.biotechadv.2024.108308 · Cited by 84
Sustainability of the Dairy Industry: Emissions and Mitigation Opportunities
Peterson, C. B., & Mitloehner, F. M. (2021) · Frontiers in Animal Science · doi:10.3389/fanim.2021.760310 · Cited by 73
Recent advancement and applications of biochar technology as a multifunctional component towards sustainable environment
Amalina, F., Krishnan, S., Zularisam, A. W., & Nasrullah, M. (2023) · Environmental Development · doi:10.1016/j.envdev.2023.100819 · Cited by 62
A Meta-Analysis of Essential Oils Use for Beef Cattle Feed: Rumen Fermentation, Blood Metabolites, Meat Quality, Performance and, Environmental and Economic Impact
Orzuna‐Orzuna, J. F., Dorantes-Iturbide, G., Lara-Bueno, A., Miranda‐Romero, L. A., Martínez, G. D. M., & Santiago-Figueroa, I. (2022) · Fermentation · doi:10.3390/fermentation8060254 · Cited by 61
Feed additives for methane mitigation: Recommendations for testing enteric methane-mitigating feed additives in ruminant studies
Hristov, A. N., Bannink, A., Battelli, M., Belanche, A., Sanz, M. C. C., Fernández-Turren, G., García, F. J. G., Jonker, A., Kenny, D. A., Lind, V., et al. (2024) · Journal of Dairy Science · doi:10.3168/jds.2024-25050 · Cited by 67
Self-citation
Insects as alternative feed for ruminants: comparison of protein evaluation methods
Toral, P. G., Hervás, G., González-Rosales, M. G., Mendoza, A. G., Jimenez, L. E. R., & Frutos, P. (2022) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-021-00671-2 · Cited by 49
Prediction of nitrogen excretion from data on dairy cows fed a wide range of diets compiled in an intercontinental database: A meta-analysis
Bougouin, A., Hristov, A. N., Dijkstra, J., Aguerre, M. J., Ahvenjärvi, S. A., Arndt, C., Bannink, A., Bayat, A. R., Benchaar, C., Boland, T. M., et al. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-20885 · Cited by 48
Self-citation
Proxy Measures and Novel Strategies for Estimating Nitrogen Utilisation Efficiency in Dairy Cattle
Lavery, A., & Ferris, C. P. (2021) · Animals · doi:10.3390/ani11020343 · Cited by 46
Updating analysis of nitrogen balance experiments in dairy cows
Spanghero, M., & Kowalski, Z. M. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19656 · Cited by 46
Differences between Holstein dairy cows in renal clearance rate of urea affect milk urea concentration and the relationship between milk urea and urinary nitrogen excretion
Müller, C. B. M., Görs, S., Derno, M., Tuchscherer, A., Wimmers, K., Zeyner, A., & Kuhla, B. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.143198 · Cited by 44
Unraveling the pros and cons of various in vitro methodologies for ruminant nutrition: a review
Vinyard, J. R., & Faciola, A. P. (2022) · Translational Animal Science · doi:10.1093/tas/txac130 · Cited by 43
Integrating Omics Technologies for a Comprehensive Understanding of the Microbiome and Its Impact on Cattle Production
Kaur, H., Kaur, G., Gupta, T., Mittal, D., & Ali, S. A. (2023) · Biology · doi:10.3390/biology12091200 · Cited by 47
Effects of dietary rumen–protected Lys levels on rumen fermentation and bacterial community composition in Holstein heifers
Kong, F., Gao, Y., Tang, M., Fu, T., Diao, Q., Bi, Y., & Tu, Y. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10684-y · Cited by 41
Nutritional Value of the Larvae of the Black Soldier Fly (Hermetia illucens) and the House Fly (Musca domestica) as a Food Alternative for Farm Animals—A Systematic Review
da Silva, W. C., da Silva, É. B. R., da Silva, J. A. R., Martorano, L. G., Belo, T. S., Sousa, C. E. L., Júnior, R. N. C. C., Andrade, R. L., Santos, A. G. D. S., de Carvalho, K. C., et al. (2024) · Insects · doi:10.3390/insects15080619 · Cited by 43
Meta-analysis and sustainability of feeding slow-release urea in dairy production
Salami, S. A., Moran, C. A., Warren, H. E., & Taylor-Pickard, J. A. (2021) · PLoS ONE · doi:10.1371/journal.pone.0246922 · Cited by 36
Rumen microbiome associates with postpartum ketosis development in dairy cows: a prospective nested case–control study
Kong, F., Wang, S., Zhang, Y., Li, C., Dai, D., Guo, C., Wang, Y., Cao, Z., Yang, H., Bi, Y., et al. (2025) · Microbiome · doi:10.1186/s40168-025-02072-3 · Cited by 39
Effect of Cereals and Legumes Processing on In Situ Rumen Protein Degradability: A Review
Iommelli, P., Zicarelli, F., Musco, N., Sarubbi, F., Grossi, M., Lotito, D., Lombardi, P., Infascelli, F., & Tudisco, R. (2022) · Fermentation · doi:10.3390/fermentation8080363 · Cited by 32
Production performance of Holstein cows at 4 stages of lactation fed 4 dietary crude protein concentrations
Letelier, P., Zanton, G. I., & Wattiaux, M. A. (2022) · Journal of Dairy Science · doi:10.3168/jds.2022-22146 · Cited by 27
Manure Valorization Using Black Soldier Fly Larvae: A Review of Current Systems, Production Characteristics, Utilized Feed Substrates, and Bioconversion and Nitrogen Conversion Efficiencies
Grassauer, F., Ferdous, J., & Pelletier, N. L. (2023) · Sustainability · doi:10.3390/su151612177 · Cited by 30
Whole-lactation feed intake, milk yield, and energy balance of Holstein and Swedish Red dairy cows fed grass-clover silage and 2 levels of byproduct-based concentrate
Karlsson, J., Lindberg, M., Åkerlind, M., & Holtenius, K. (2020) · Journal of Dairy Science · doi:10.3168/jds.2020-18204 · Cited by 27
Nutrición animal: Texto de formación universitaria
Huanca, B. R. (2023) · Instituto Universitario de Innovación Ciencia y Tecnología Inudi Perú eBooks · doi:10.35622/inudi.b.090 · Cited by 26
Predicting ruminally undegraded and microbial protein flows from the rumen
Hanigan, M. D., de Souza, V. C., Martineau, R., Daley, V. L., & Kononoff, P. J. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19672 · Cited by 27
Altering Dietary Soluble Protein Levels With Decreasing Crude Protein May Be a Potential Strategy to Improve Nitrogen Efficiency in Hu Sheep Based on Rumen Microbiome and Metabolomics
Zhang, Z., Shahzad, K., Shen, S., Dai, R., Lu, Y., Lu, Z., Li, C., Chen, Y., Qi, R., Gao, P., et al. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2021.815358 · Cited by 24
Enhancing Metabolic Efficiency through Optimizing Metabolizable Protein Profile in a Time Progressive Manner with Weaned Goats as a Model: Involvement of Gut Microbiota
Wu, J., Zhang, X., Wang, M., Zhou, C., Jiao, J., & Tan, Z. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02545-21 · Cited by 22
Effect of Tannin Inclusion on the Enhancement of Rumen Undegradable Protein of Different Protein Sources
Loregian, K. E., Pereira, D. A. B., Rigon, F., Magnani, E., Marcondes, M. I., Baumel, E. A., Branco, R. H., Benedeti, P. D. B., & de Paula, E. M. (2023) · Ruminants · doi:10.3390/ruminants3040034 · Cited by 22
Reducing Ruminal Ammonia Production With Improvement in Feed Utilization Efficiency and Performance of Murrah Buffalo (Bubalus bubalis) Through Dietary Supplementation of Plant-Based Feed Additive Blend
Chanu, Y. M., Paul, S. S., Dey, A., & Dahiya, S. S. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.00464 · Cited by 23
Scenario Analysis of Livestock Carrying Capacity Risk in Farmland from the Perspective of Planting and Breeding Balance in Northeast China
Zhao, Z., Deng, X., Zhang, F., Li, Z., Shi, W., Sun, Z., & Zhang, X. (2022) · Land · doi:10.3390/land11030362 · Cited by 21
Effect of rumen-protected lysine supplementation of diets based on corn protein fed to lactating dairy cows
Lobos, N. E., Wattiaux, M. A., & Broderick, G. A. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19835 · Cited by 21
Rumen Degradability of Barley, Oats, Sorghum, Triticale, and Wheat In Situ and the Effect of Pelleting
Pan, L., Huang, K. H., Middlebrook, T., Zhang, D., Bryden, W. L., & Li, X. (2021) · Agriculture · doi:10.3390/agriculture11070647 · Cited by 20
Microbial insights into ruminal fiber degradation and feed efficiency of Hu sheep
Xu, H., Wang, G., Gao, Q., Liu, Z., Jia, J., Xu, Y., Chen, Z., Li, B., & Li, C. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1561336 · Cited by 21
Empirical Evaluation and Prediction of Protein Requirements for Maintenance and Growth of 18–24 Months Old Thai Swamp Buffaloes
Paengkoum, S., Tatsapong, P., Taethaisong, N., Thongpea, S., Purba, R. A. P., & Paengkoum, P. (2021) · Animals · doi:10.3390/ani11051405 · Cited by 17
Digestive and metabolic efficiency of energy and nitrogen during lactation and the dry period in dairy cows
Daniel, J., Sanz-Fernandez, M. V., Nichols, K., Doelman, J., & Martín‐Tereso, J. (2022) · Journal of Dairy Science · doi:10.3168/jds.2022-22142 · Cited by 18
The Flourishing Camel Milk Market and Concerns about Animal Welfare and Legislation
Smits, M. G., Joosten, H. M. L. J., Faye, B., & Burger, P. A. (2022) · Animals · doi:10.3390/ani13010047 · Cited by 21
Strategies for Healthier Meat Foods: An Overview
Espinales, C., Baldeón, M., Bravo, C., Toledo, H. D. L. G., Carballo, J., Romero-Peña, M., & Cáceres, P. J. (2024) · Preventive Nutrition and Food Science · doi:10.3746/pnf.2024.29.1.18 · Cited by 18
The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport
Liebe, F., Liebe, H., Kaessmeyer, S., Sponder, G., & Stumpff, F. (2020) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-020-02393-2 · Cited by 20
Reducing crude protein content in the diet of lactating dairy cows improved nitrogen-use-efficiency and reduced N excretion in urine, whilst having no obvious effects on the rumen microbiome
Zeleke, A. W., Dimonaco, N. J., Lawther, K., Lavery, A., Ferris, C. P., Moorby, J. M., & Huws, S. (2025) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-025-01240-7 · Cited by 23
Metabolizable Protein: 2. Requirements for Maintenance in Growing Saanen Goats
Souza, A. P., Vargas, J. A. C., Fernandes, M. H. M. R., Almeida, A. K., de Resende, K. T., & Teixeira, I. A. M. D. A. (2021) · Frontiers in Veterinary Science · doi:10.3389/fvets.2021.650203 · Cited by 17
Defatted silkworm pupae meal as an alternative protein source for cattle
Rashmi, K. M., Chandrasekharaiah, M., Soren, N. M., Prasad, K., David, C. G., Thirupathaiah, Y., & Shivaprasad, V. (2022) · Tropical Animal Health and Production · doi:10.1007/s11250-022-03323-3 · Cited by 17
Intake, digestibility, nitrogen balance and performance in lamb fed spineless cactus silage associated with forages adapted to the semiarid environment Spineless cactus silages in diets for lambs
Silva, T. S., de Araújo, G. G. L., Santos, E. M., de Oliveira, J. S., Godoi, P. F. A., Góis, G. C., Perazzo, A. F., Ribeiro, O. L., Turco, S. H. N., & Campos, F. S. (2023) · Livestock Science · doi:10.1016/j.livsci.2023.105168 · Cited by 17
Effect of nitrate supplementation, dietary protein supply, and genetic yield index on performance, methane emission, and nitrogen efficiency in dairy cows
Wang, W., Lund, P., Larsen, M., & Weisbjerg, M. R. (2023) · Journal of Dairy Science · doi:10.3168/jds.2022-22906 · Cited by 19
Research updates and progress on nutritional significance of the amides I and II, alpha-helix and beta-sheet ratios, microbial protein synthesis, and steam pressure toasting condition with globar and synchrotron molecular microspectroscopic techniques with chemometrics
Rodríguez‐Espinosa, M. E., Guevara-Oquendo, V. H., He, J., Zhang, W., & Yu, P. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2274442 · Cited by 17
Graduate Student Literature Review: Concepts and challenges of amino acid supply and nitrogen metabolism in dairy cattle
de Oliveira, M. H., Costa, C., & Fernandes, T. (2025) · Journal of Dairy Science · doi:10.3168/jds.2024-26136 · Cited by 23
Sericea lespedeza (Lespedeza juncea var. sericea) for sustainable small ruminant production: Feed, helminth suppressant and meat preservation capabilities
Mahachi, L. N., Chikwanha, O. C., Katiyatiya, C. L. F., Marufu, M. C., Aremu, A. O., & Mapiye, C. (2020) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2020.114688 · Cited by 16
Essential oil from Arnica montana on feedlot performance, ingestive behavior, carcass characteristics, rumen morphometrics characteristics and meat fatty acids profile of lambs
Dias, P. C. G., dos Santos, I. J., da Silva, A. L. A., de Assis, R. G., Vicente, A. C. S., Carlis, M. S. D. P., Soares, L. C. B., Comelli, J. H., Biava, J. S., de Araújo, R. C., et al. (2023) · Small Ruminant Research · doi:10.1016/j.smallrumres.2023.106920 · Cited by 17
The Effects of Short-Time Delayed Sealing on Fermentation, Aerobic Stability and Chemical Composition on Maize Silages
de Melo, N. N., Carvalho-Estrada, P. D. A., Tavares, Q. G., Pereira, L. D. M., Delai Vigne, G. L., Camargo Rezende, D. M. L., & Schmidt, P. (2023) · Agronomy · doi:10.3390/agronomy13010223 · Cited by 15
Platforms to accelerate biomanufacturing of enzyme and probiotic animal feed supplements: discovery considerations and manufacturing implications
Speight, R. E., Navone, L., Gebbie, L. K., Blinco, J.-A., & Bryden, W. L. (2022) · Animal Production Science · doi:10.1071/an21342 · Cited by 15
Effects of calcium ammonium nitrate fed to dairy cows on nutrient intake and digestibility, milk quality, microbial protein synthesis, and ruminal fermentation parameters
Almeida, K. V., dos Santos, G. T., Daniel, J. L. P., de Osorio, J. A. C., Yamada, K. L. G., SIPPERT, M. R., Cabral, J. F., Marchi, F. E. D., de Araújo, R. C., & Vyas, D. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21124 · Cited by 18
Review: Problems in determining metabolisable protein value of dairy cow diets and the impact on protein feeding
Huhtanen, P. J., & Ahvenjärvi, S. A. (2022) · animal · doi:10.1016/j.animal.2022.100539 · Cited by 15
Self-citation
Dietary supplementation with live or autolyzed yeast: Effects on performance, nutrient digestibility, and ruminal fermentation in dairy cows
Takiya, C. S., Chesini, R. G., de Freitas, A. C., Grigoletto, N. T. S., Vieira, D. J. C., Poletti, G., Martins, N. P., Sbaralho, O. P., Roth, N., Acedo, T. S., et al. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24194 · Cited by 17
Effects of tanniferous sainfoin and Acacia mearnsii extract on urinary N excretion and ammonia volatilization from the slurry of dairy cows
Lazzari, G., Münger, A., Heimo, D., Seifert, J., Camarinha‐Silva, A., Borda-Molina, D., Zähner, M., Schrade, S., Kreuzer, M., & Dohme‐Meier, F. (2023) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2023.115577 · Cited by 15
Self-citation
Impact of oil type and savory plant on nutrient digestibility and rumen fermentation, milk yield, and milk fatty acid profile in dairy cows
Golbotteh, M. M., Malecky, M., Aliarabi, H., & Zamani, P. (2024) · Scientific Reports · doi:10.1038/s41598-024-73138-9 · Cited by 15
Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose
Ungerfeld, E. M., Aedo, M. F., Muñoz, C., Urrutia, N. L., Martínez, E. D., & Saldivia, M. (2020) · Microorganisms · doi:10.3390/microorganisms8060799 · Cited by 13
Increased Nitrogen Retention and Reduced Methane Emissions of Beef Cattle Grazing Legume vs. Grass Irrigated Pastures in the Mountain West USA
MacAdam, J. W., Pitcher, L. R., Bolletta, A. I., Guevara, R. D., Beauchemin, K. A., Dai, X., & Villalba, J. J. (2022) · Agronomy · doi:10.3390/agronomy12020304 · Cited by 14
Benzoic acid supplementation improves the growth performance, nutrient digestibility and nitrogen metabolism of weaned lambs
Zhang, W., Sun, S., Zhang, Y., Zhang, Y., Wang, J., Liu, Z., & Kailun, Y. (2024) · Frontiers in Veterinary Science · doi:10.3389/fvets.2024.1351394 · Cited by 12
Synthesis and characterization of sewage sludge biochar toward adsorptive removal of pollutants for sustainability: a review
Soni, N. K., Yadav, K., Shrivastav, P., & Bhale, P. V. (2025) · Journal of Thermal Analysis and Calorimetry · doi:10.1007/s10973-025-14065-3 · Cited by 12
Ruminant meat production and quality enhancement, nematode suppression and greenhouse gas emission mitigation: A sustainable paradigm for valorisation of Acacia leaves
Uushona, T., Chikwanha, O. C., Katiyatiya, C. L. F., Tayengwa, T., Strydom, P. E., & Mapiye, C. (2021) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2021.115187 · Cited by 13
Licuri oil improves feedlot performance and modifies ruminal fauna of Santa Inês ewes
Cavalcanti, C. P. L., Macedo, T. J. S., Góis, G. C., Menezes, V. G., do Monte, A. P. O., da Silva, A. D., da Silva, D. J. M., da Silva, E. O., de Araújo, G. G. L., Rodrigues, R. T. D. S., et al. (2022) · Livestock Science · doi:10.1016/j.livsci.2022.105093 · Cited by 12
Genetic analysis of milk urea concentration and its genetic relationship with selected traits of interest in dairy cows
Chen, Y., Atashi, H., Vanderick, S., Mota, R. R., Soyeurt, H., Hammami, H., & Gengler, N. (2021) · Journal of Dairy Science · doi:10.3168/jds.2021-20659 · Cited by 12
Slow-Release Urea Technologies: Effects on Nitrogen Utilization and Small Ruminant Performance
Bezerra, L. R., Mazza, P., de Lucena, K. H. D. O. S., Amorim, M. A. F., de Moura, J. F. P., de Oliveira, J. P. F., Oliveira, R. L., Barbosa, A. M., Fonseca, M. A., & Filho, J. M. P. (2025) · Ruminants · doi:10.3390/ruminants5040050 · Cited by 12
Effects of plant extracts from Stryphnodendron adstringens (mart.) coville, Lafoensia pacari a. st.‐hil, copaifera spp., and Pterodon emarginatus Vogel on in vitro rumen fermentation
Lemos, B. J. M., de Souza, F. M., Arnhold, E., da Conceição, E. C., Couto, V. R. M., & Fernandes, J. J. D. R. (2021) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.13502 · Cited by 11
Greenhouse gas emissions and crop-specific emission factors of eight upland crops based on a six-year field experiment in the North China Plain
Yang, X., Li, S., Du, T., Kang, S., Siddique, K. H. M., & Butterbach‐Bahl, K. (2024) · Sustainable Production and Consumption · doi:10.1016/j.spc.2024.08.013 · Cited by 11
Changing the grazing session from morning to afternoon or including tannins in the diet was effective in decreasing the urinary nitrogen of dairy cows fed a total mixed ration and herbage
Pozo, C. A., Kozloski, G. V., Cuffia, M., Repetto, J. L., & Cajarville, C. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21149 · Cited by 13
OsbZIP1 regulates phosphorus uptake and nitrogen utilization, contributing to improved yield
Tanaka, N., Yoshida, S., Islam, M. S., Yamazaki, K., Fujiwara, T., & Ohmori, Y. (2024) · The Plant Journal · doi:10.1111/tpj.16598 · Cited by 11
Effects of graded levels of dietary pomegranate peel on methane and nitrogen losses, and metabolic and health indicators in dairy cows
Niu, P., Kreuzer, M., Liesegang, A., Kunz, C., Schwarm, A., & Giller, K. (2023) · Journal of Dairy Science · doi:10.3168/jds.2022-23141 · Cited by 10
Self-citation
Meta-analysis of the effects of essential oils on consumption, performance, and ruminal fermentation of beef cattle
Ferreira, F. D. J., FERNANDES, L. D., Júnior, A. R. L., Rosado, G. L., & Bento, C. B. P. (2024) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2024.115956 · Cited by 14
Rumen-protected methionine and lysine supplementation to the low protein diet improves animal growth through modulating colonic microbiome in lambs
Luo, Z., Ou, H., Tan, Z., & Jiao, J. (2025) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-025-01183-z · Cited by 14
Single-variant genome-wide association study and regional heritability mapping of protein efficiency and performance traits in Large White pigs
Ewaoluwagbemiga, E. O., Lloret‐Villas, A., Nosková, A., Pausch, H., & Kasper, C. (2025) · Genetics Selection Evolution · doi:10.1186/s12711-025-00993-z · Cited by 10
Effects of protein sources and inclusion levels on nitrogen metabolism and urea kinetics of Nellore feedlot steers fed concentrate-based diets
de Souza, V. C., Messana, J. D., Batista, E. D., Alves, K. L. G. C., Titgemeyer, E. C., Pires, A. V., Ferraz, M. V. D. C., da Silva, L. G., Negrão, J. A., Costa, V. E., et al. (2021) · Journal of Animal Science · doi:10.1093/jas/skab185 · Cited by 12
Effects of faba bean, blue lupin and rapeseed meal supplementation on nitrogen digestion and utilization of dairy cows fed grass silage-based diets
Kuoppala, K., Jaakkola, S., Garry, B., Ahvenjärvi, S. A., & Rinne, M. (2021) · animal · doi:10.1016/j.animal.2021.100300 · Cited by 10
Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
Pereira, T. C. D. J., Pereira, M. L. A., de Carvalho, G. G. P., Silva, H. G. D. O., dos Santos, A. B., Pina, D. D. S., & Sousa, L. B. (2022) · Animals · doi:10.3390/ani12091195 · Cited by 9
Essential oil from Arnica montana alters the protein metabolism in lambs fed with high-concentrate diets
Dias, P. C. G., dos Santos, I. J., Gasparina, J. M., Bandoria, N. D. A., Soares, L. C. B., da Silva, A. L. A., de Assis, R. G., Polizel, D. M., Biava, J. S., Pires, A. V., et al. (2022) · Small Ruminant Research · doi:10.1016/j.smallrumres.2022.106894 · Cited by 9
Quantifying the Contributing Potential of European Grasslands to Food Protein and Organic Manure in a Circular Food System
Pfeifer, C., Winterberg, R., & Leiber, F. (2025) · Grass and Forage Science · doi:10.1111/gfs.12713 · Cited by 9
Host-specific microbiome-rumination interactions shape methane-yield phenotypes in dairy cattle
Castaneda, A., Indugu, N., Lenker, K., Narayan, K. S., Rassler, S., Bender, J. S., Baker, L. D., Purandare, O., Chai, D., Zhao, X., et al. (2025) · mSphere · doi:10.1128/msphere.00090-25 · Cited by 9
A multiparametric approach to assessing residual pain experienced by dairy cows undergoing digestive tract surgery under multimodal analgesia
Durand, D., Fauré, M., Lamberton, P., Lemosquet, S., & Roches, A. D. B. D. (2021) · animal · doi:10.1016/j.animal.2021.100338 · Cited by 9
Ability of three dairy feed evaluation systems to predict postruminal outflows of nitrogenous compounds in dairy cows: A meta-analysis
Martineau, R., Ouellet, D. R., Pellerin, D., Firkins, J. L., Hanigan, M. D., White, R. R., LaPierre, P. A., Amburgh, M. E. V., & Lapierre, H. (2023) · Journal of Dairy Science · doi:10.3168/jds.2022-23215 · Cited by 9
Effect of rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation
Rosmalia, A., Astriani, A., Sahroni, W. P., Permana, I. G., & Despal, D. (2022) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/951/1/012013 · Cited by 10
Physical effectiveness of corn silage fractions stratified with the Penn State Particle Separator for lactating dairy cows
Filho, F. A. P., Bragatto, J. M., Parra, C. S., Silva, S. M. S., Roco, P. J., Ferraretto, L. F., Pereira, M. N., & Daniel, J. L. P. (2023) · Journal of Dairy Science · doi:10.3168/jds.2022-23063 · Cited by 9
Phytochemical Composition and Effects of Aqueous Extracts from Moringa oleifera Leaves on In Vitro Ruminal Fermentation Parameters
de Oliveira, I. S. T., Fernandes, T., Santos, A. R. D., Aquino, C. G., Brítez, G. D. V., & de Vargas, F. M. (2025) · Ruminants · doi:10.3390/ruminants5010004 · Cited by 8
Invited review: Perspectives on nitrogen in dairy cattle nutrition
Hristov, A. N., Huhtanen, P. J., van Duinkerken, G., & Pacheco, D. (2026) · Journal of Dairy Science · doi:10.3168/jds.2025-27276 · Cited by 8
Self-citation
Effect of wilting and lactic acid bacteria inoculant on in situ and in vitro determined protein value of grass silages
Rupp, C., Westreicher‐Kristen, E., & Susenbeth, A. (2021) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2021.115115 · Cited by 8
Dry malt extract from barley partially replacing ground corn in diets of dairy cows: Nutrient digestibility, ruminal fermentation, and milk composition
Bugoni, M., Takiya, C. S., Grigoletto, N. T. S., Nunes, A. T., Vittorazzi, P. C., Chesini, R. G., da Silva, G. G., de Alcantara, L. B., Rennó, L. N., & Rennó, F. P. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21682 · Cited by 8
No size‐dependent net particle retention in the hindgut of horses
Schwarm, A., Clauß, M., Ortmann, S., & Jensen, R. B. (2022) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.13757 · Cited by 8
Self-citation
Feed intake, digestibility, and energy contents in growing bull fed pineapple crop waste silage in different planes of nutrition
de Mello, B. L. B., Fernandes, A. M., de Oliveira, T. S., Leonel, F. D. P., Glória, L. S., & Silva, R. S. T. (2021) · Tropical Animal Health and Production · doi:10.1007/s11250-021-02640-3 · Cited by 8
Digestibility of starch, ruminal protozoa count, and blood parameters of Dorper lambs with differences in residual feed intake
Ramos, E. J. N., Matos, J. C., Ferreira, B. J. M., Rodrigues, J. D. P. S., Félix, W. P., Menezes, D. R., Góis, G. C., Rodrigues, R. T. D. S., & Queiróz, M. A. Á. (2021) · Small Ruminant Research · doi:10.1016/j.smallrumres.2021.106415 · Cited by 8
Analysis of crude protein utilisation in ruminant rations: supplementation of limiting amino acids and their effect on the environment – an updated review
Sajid, Q. U. A., Wilk, M., & Asghar, M. U. (2023) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/166576/2023 · Cited by 7
Deciphering Hyperammonia-Producing Bacteria (HAB) in the Rumen of Water Buffaloes (Bubalus bubalis) and Their Inhibition through Plant Extracts and Essential Oils
Chanu, Y. M., Paul, S. S., Dey, A., & Jerome, A. (2024) · Microorganisms · doi:10.3390/microorganisms12102040 · Cited by 7
Enhancing nutrient efficiency through optimizing protein levels in lambs: Involvement of gastrointestinal microbiota
Luo, Z., Ou, H., McSweeney, C. S., Tan, Z., & Jiao, J. (2024) · Animal nutrition · doi:10.1016/j.aninu.2024.09.006 · Cited by 9
Leveraging Gastrointestinal Microbiota‒Host Co-oscillation Patterns for Improving Nitrogen Utilization Efficiency in Ruminants: A Review
Jiao, J., Zhao, Y., & Tan, Z. (2025) · Journal of Nutrition · doi:10.1016/j.tjnut.2025.04.001 · Cited by 8
Rumen-Protected Methionine Supplementation in the Diet Improved the Production Performance of Dairy Goats by Optimizing the Amino Acid Profile and Lipid Metabolism and Modulating the Colonic Microbiome
Jiang, X., Wang, J., Zhang, Y., Li, J., Liu, H., Wu, S., & Yao, J. (2025) · Animals · doi:10.3390/ani15233386 · Cited by 7
Metabolomics and amino acid profiling of plasma reveals the metabolite profiles associated with nitrogen utilisation efficiency in primiparous dairy cows
Li, H., La, S. K., Zhang, L., Li, S., Yu, Z., Ao, L. M., Gao, T., & Huang, H. T. (2024) · animal · doi:10.1016/j.animal.2024.101202 · Cited by 7
Self-citation
Body weight and sex effects on digesta mean retention time in growing Saanen goats
Gindri, M., Leite, R. F., Härter, C. J., da Silva, S. P., St-Pierre, N. R., Fernandes, M. H. M. R., Berchielli, T. T., & Teixeira, I. A. M. D. A. (2020) · Translational Animal Science · doi:10.1093/tas/txaa028 · Cited by 7
Legumes for animal nutrition and dietary energy
Solomon, J. K. Q. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85797-0.00026-4 · Cited by 7
In vitro protein fractionation methods for ruminant feeds
Tunkala, B. Z., DiGiacomo, K., Alvarez-Hess, P. S., Dunshea, F. R., & Leury, B. J. (2023) · animal · doi:10.1016/j.animal.2023.101027 · Cited by 7
Nutrient digestibility and balance studies
Mesgaran, S. D., Kuhla, B., Baumont, R., Cantalapiedra-Hijar, G., Nozière, P., Lund, P., Humphries, D. J., & Dijkstra, J. (2020) · doi:10.5680/mcpb007 · Cited by 7
Self-citation
Evaluation of Molly model predictions of ruminal fermentation, nutrient digestion, and performance by dairy cows consuming ryegrass-based diets
Morales, A. G., Vibart, R. E., Li, M., Jonker, A., Pacheco, D., & Hanigan, M. D. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19740 · Cited by 7

Authors: Hristov, A. N., Bannink, A., Crompton, L. A., Huhtanen, P., Kreuzer, M., McGee, M., Nozière, P., Reynolds, C. K., Bayat, A. R., Yáñez-Ruiz, D. R., Dijkstra, J., Kebreab, E., Schwarm, A., Shingfield, K. J., & Yu, Z.
Affiliations: Pennsylvania State University (US)
Wageningen University & Research (NL)
University of Reading (GB)
Swedish University of Agricultural Sciences (SE)
ETH Zurich (CH)
Teagasc - The Irish Agriculture and Food Development Authority (IE)
Université Clermont Auvergne (FR)
Natural Resources Institute Finland (FI)
University of California, Davis (US)
Aberystwyth University (GB)
The Ohio State University (US)
ORCID: Hristov, A.N.
Bannink, A.
Crompton, L.A.
Huhtanen, P.
Kreuzer, M.
Nozière, P.
Reynolds, C.K.
Bayat, A.R.
Dijkstra, J.
Kebreab, E.
Schwarm, A.
Yu, Z.
Venue: Journal of Dairy Science
Publisher: American Dairy Science Association
Published: 2019-07
Volume: 102
Issue: 7
Pages: 5811-5852
ISSN: 0022-0302 (ISSN-L)
DOI: 10.3168/jds.2018-15829
OpenAlex: W2942130108
Cited by: 283
References: 359
Citations by year: 2026: 46 · 2025: 50 · 2024: 59 · 2023: 28 · 2022: 46 · 2021: 31 · 2020: 21 · 2019: 2
FWCI: 3.16
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (am, 348 d)
Locations: Journal of Dairy Science (acceptedVersion)
PubMed (publishedVersion)
T-Stór (Teagasc) (acceptedVersion, cc-by-nc-sa)
HAL (submittedVersion)
CentAUR (University of Reading) (submittedVersion)
Arrow@dit (Dublin Institute of Technology) (submittedVersion, cc-by-nc-sa)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by-nc-nd)
Jukuri (Natural Resources Institute Finland (Luke)) (submittedVersion)
Socio-Environmental Systems Modeling (submittedVersion, cc-by-nc-nd)
Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Funders: U.S. Department of Agriculture
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (MIT01-GLOBALNET-EEZ)
Pennsylvania State University
Ministerie van Landbouw, Natuur en Voedselkwaliteit
National Institute of Food and Agriculture (2014-67003-21979, NH00616-R)
Topics: Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Health
Keywords: rumen microbial protein synthesis, urea recycling, ammonia emissions, nitrous oxide emissions, environmental impact of livestock, nitrogen use efficiency
MeSH terms: Animals, Animal Feed, Animal Husbandry, Animal Nutritional Physiological Phenomena, Nitrogen, Ruminants, Animal Nutrition Sciences

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks