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Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community
Open accessAbstract
Different inclusion rates of oregano essential oil (OEO) were investigated for their effects on ruminal in vitro fermentation parameters, total gas, methane production, and bacterial communities. Treatments were (1) control, 0 mg/L of OEO (CON); 13 mg/L (OEO1); 52 mg/L (OEO2); 91 mg/L (OEO3); and 130 mg/L (OEO4), each incubated with 150 mL of buffered rumen fluid and 1,200 mg of substrate for 24 h using the Ankom in vitro gas production system (Ankom Technology Corp., Fairport, NY). Treatment responses were statistically analyzed using polynomial contrasts. Digestibility of DM, NDF, and ADF increased quadratically with increasing OEO inclusion rates. Digestibility of DM and NDF were highest for OEO2, whereas ADF digestibility was highest for OEO3, compared with CON, with the remaining treatments being intermediate and similar. Ammonia nitrogen concentrations decreased from CON at a quadratic rate with increasing OEO inclusion rates, and OEO2 had the lowest concentration compared with the other groups. Total VFA, acetate, propionate, butyrate, valerate, and isovalerate concentrations linearly decreased with increasing OEO inclusion rates. Total gas production levels by CON and OEO4 were greater than those of OEO1, OEO2, and OEO3 in a quadratic response, and methane production linearly decreased from CON, compared with OEO4, at a decreasing rate with OEO inclusion rates. As determined by 16S rRNA sequencing, the α biodiversity of ruminal bacteria was similar among OEO inclusion rates. Increasing OEO inclusion rates linearly increased the relative abundance of Prevotella and Dialister bacteria. Several bacteria demonstrated different polynomial responses, whereas several bacteria were similar among increasing OEO inclusion rates. These results suggested that OEO supplementation can modify ruminal fermentation to alter VFA concentrations and reduce methane emissions by extensively altering the ruminal bacterial community, suggesting an optimal feeding rate for future animal studies of approximately 52 mg/L for mature ruminants.
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“Recently, Zhou et al. (239) suggested that oregano EO (52 mg/L) in mature ruminants can modify ruminal fermentation and mitigate in vitro CH4 production through mediating ruminal bacteria (Prevotella and Dialister).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [239]
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Role of Secondary Plant Metabolites on Enteric Methane Mitigation in Ruminants
Kú-Vera, J. C., Jiménez‐Ocampo, R., Valencia-Salazar, S. S., Montoya-Flores, M. D., Molina-Botero, I. C., Arango, J., Gómez, C., Aguilar-Pérez, C. F., & Solorio‐Sánchez, F. J. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.00584 · Cited by 300
Hassan, F.-U., Arshad, M. A., Ebeid, H. M., Rehman, M. S.-U., Khan, M. S., Shahid, S., & Yang, C. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.575801 · Cited by 186
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Arshad, M. A., Hassan, F., Rehman, M. S.-U., Huws, S., Cheng, Y., & Din, A. U. (2021) · Animal nutrition · doi:10.1016/j.aninu.2021.03.004 · Cited by 151
Metadata
- Authors
- Zhou, R., Wu, J., Lang, X., Liu, L., Casper, D. P., Wang, C., Zhang, L., & Wei, S.
- Affiliations
- Gansu Agricultural University (CN)
- Gansu Academy of Agricultural Sciences (CN)
- Corresponding author
- Wu, Jianping
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2020-03
- Volume
- 103
- Issue
- 3
- Pages
- 2303-2314
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2999305564
- Cited by
- 130
- References
- 84
- Citations by year
- 2026: 23 · 2025: 18 · 2024: 24 · 2023: 22 · 2022: 16 · 2021: 19 · 2020: 8
- FWCI
- 20.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (CARS-39, 31460592)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- oregano essential oil, rumen fermentation, methane mitigation, volatile fatty acids, in vitro gas production, forage digestibility, ammonia nitrogen, Prevotella, Dialister, ruminant feed additives, dairy cattle
- MeSH terms
- Gastrointestinal Microbiome, Ammonia, Animals, Animal Feed, Bacteria, Cattle, Diet, Digestion, Female, Fermentation, Male, Methane, Nitrogen, Oils, Volatile, Plant Oils, RNA, Ribosomal, 16S, Rumen, Sheep, Origanum
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