Opsonic Activity of Bovine Serum and Mammary Secretion After Escherichia coli J5 Vaccination
Open accessAbstract
Six pairs of cows were used to determine the effects of immunization with an Escherichia coli (O111:B4) J5 bacterin on in vitro opsonization of a smooth heterologous strain of E. coli. One cow in each pair was either immunized with the vaccine or sham-immunized at drying off, 30 d after drying off, and at calving. Opsonizing bacteria with serum collected from vaccinated cows 21 d after calving resulted in higher mean number of intracellular bacteria per phagocytosing neutrophil than opsonizing bacteria with serum collected from control cows. Phagocytic parameters using serum collected at drying off and calving did not differ between treatment groups. A trend for enhanced opsonic activity of colostrum from vaccinates was noted. Enhanced opsonization by serum from vaccinated cows coincided with higher serum IgM titer to E. coli J5 whole cell antigen compared with controls. Serum IgG titers to E. coli J5 did not differ between groups. Colostrum IgG titers to E. coli J5 were greater at calving in vaccinated than in control cows. Colostrum and milk collected 21 d after calving from vaccinated cows had higher IgM titers to E. coli J5 than did mammary secretions from control cows. Numbers of intracellular bacteria per phagocytizing neutrophil were correlated positively with IgM titers to E. coli J5 in both serum and colostrum.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Enhanced opsonization of wild strains of coliform bacteria by serum and whey of vaccinated cows was correlated with elevated IgM titers.
“Enhanced opsonization of wild strains of coliform bacteria by serum and whey of vaccinated cows was correlated with elevated IgM titers [30].”
From Coliform mastitis (2003) · cites this paper as [30]
The Serum section reports increased opsonization with serum collected 21 days after calving and a correlation with IgM titers. The Discussion reports an IgM-related trend toward increased opsonization with colostrum, but mammary secretions collected at other times did not show increased opsonic activity. The study tested one heterologous E. coli strain, so the claim overgeneralizes to wild coliform strains and to whey without the timing and uncertainty qualifiers. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Environmental Mastitis: Cause, Prevalence, Prevention
Smith, K. L., Todhunter, D. A., & Schoenberger, P. S. (1985) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(85)80993-0 · Cited by 475 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 2
- Carlson, G. P., and J. J. Kaneko. 1973. Isolation of leukocytes from bovine peripheral blood. Proc. Soc. Exp. BioI. Med. 142:853.
- Ziegler, E. J., C. J. Fisher, C. L. Sprung, R. C. Straube, J. C. Sadoff, G. E. Foulke, C. H. Wortel, M. P. Fink, R. P. Dellinger, N.N.H. Teng. r. E. Allen, H. J. Berger, G. L. Knatterud, A. F. LoBuglio. C. R. Smith, and the HA-IA Sepsis Study Group. 1991. Treatment of gram-negative bacteremia and septic shock with HA-IA human monoclonal antibody against endotoxin. New England J. Med. 324:429. Journal of Daily Science Vol. 75, No. I, 1992
Cited by
Sorted by citations
Hogan, J., & Larry Smith, K. (2003) · Veterinary Research · doi:10.1051/vetres:2003022 · Cited by 245 · see citing works
Self-citationField trial to determine efficacy of an Escherichia coli J5 mastitis vaccine
Hogan, J. S., Smith, K. L., Todhunter, D. A., & Schoenberger, P. S. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77741-8 · Cited by 96 · see citing works
Self-citationEfficacy of an Escherichia coli J5 mastitis vaccine in an experimental challenge trial.
Hogan, J. S., Weiss, W. P., Todhunter, D. A., Smith, K. L., & Schoenberger, P. S. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77777-7 · Cited by 86 · see citing works
Self-citation
- Authors
- Hogan, J. S., Todhunter, D. A., Tomita, G. M., Smith, K. L., & Schoenberger, P. S.
- Affiliations
- The Ohio State University (US)
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 1992-01
- Volume
- 75
- Issue
- 1
- Pages
- 72-77
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2092736846
- Cited by
- 22
- References
- 1
- Citations by year
- 2023: 1 · 2021: 1 · 2020: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ohio State University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Health Sciences › Veterinary › Small Animals › Animal health and immunology
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- opsonization, bovine serum, colostrum, mammary secretion, IgM, IgG, neutrophil phagocytosis, intracellular bacteria, dairy cattle, heterologous strains, dry-off, calving
- MeSH terms
- Animals, Antibodies, Bacterial, Bacterial Vaccines, Cattle, Colostrum, Escherichia coli, Escherichia coli Infections, Female, Immunoglobulin G, Immunoglobulin M, Mammary Glands, Animal, Mastitis, Bovine, Neutrophils, Opsonin Proteins, Phagocytosis, Vaccination, Escherichia coli Vaccines
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
