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Cited by 1Plant-Microbe Interactions and ImmunityAgriculture, Plant Science, Crop ManagementGrowth and nutrition in plants

Influence of Azospirillum brasilense on micro-propagated sugarcane seedlings

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Santos, Tarciana Silva dos, Ootani, Márcio Akio, Andrade, Fernanda Helena Nascimento de, Oliveira, Ronaldo Adriano de Oliveira, Guerra, Yrlania Lira, Lima, Pauliana Gomes, Romero, Melissa Alexandra, Melo, James Correia de·2023·Research, Society and Development
DOI 10.33448/rsd-v12i4.41354
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Abstract

Sugarcane is an essential crop in the Brazilian economy. The adoption of techniques in the acclimatization of plants in vitro contributes to success in the field. The present work aims to evaluate the effect of Azospirillum brasilense on the acclimatization of sugarcane seedlings. The experiment was conducted with six treatments and twelve replications. There were two control treatments without inoculation, one with N fertilization and one without, and four with A. brasilense (2.0x108 CFU) at different doses (19.92mL + N fertilization, 9.84mL, 19.92mL, and 39.84mL). Evaluations were seedling height (SH), stem diameter (SD), number of shoots per tube (NS), number of leaves (NL), fresh aerial weight (AFM), root fresh weight (RFM), total chlorophyll (TC), foliar nitrogen (FN) and visual microscopic study of the roots. The results showed that the recommended dose of A. brasilense + N fertilization increased by 36% in seedling height, 29% in stem diameter, 85% in aerial fresh mass, 21% in total chlorophyll, 142% in foliar N, and also increased root hairs and the presence of microbial biofilm. Therefore, the bacterium A. brasilense can be used in association with nitrogen fertilizers or alone, where it obtained great benefits in the increase of biomass.

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Authors
Santos, T. S. D., Ootani, M. A., Andrade, F. H. N. D., Oliveira, R. A. D. O., Guerra, Y. L., Lima, P. G., Romero, M. A., & Melo, J. C. D.
Affiliations
  • Centro de Tecnologias Estratégicas do Nordeste (BR)
  • Universidade Federal do Tocantins (BR)
ORCID
Venue
Research, Society and Development
Publisher
Research, Society and Development
Published
2023-04-18
Volume
12
Issue
4
Pages
e26312441354
ISSN
2525-3409 (ISSN-L)
DOI
10.33448/rsd-v12i4.41354
OpenAlex
W4366586897
Cited by
1
References
0
Citations by year
2025: 1
FWCI
0.16
Type
journal article
Language
en
Open access
Licence
Locations
  • Research Society and Development (publishedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (88887)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Centro de Tecnologias Estratégicas do Nordeste
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
Keywords
Azospirillum brasilense, sugarcane, Saccharum, micropropagation, acclimatization, plant growth-promoting bacteria, nitrogen fertilization, inoculum dose, seedling growth, chlorophyll content, leaf nitrogen content, root hairs, biofilm, biomass

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