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Cited by 162Essential Oils and Antimicrobial ActivitySalmonella and Campylobacter epidemiologyListeria monocytogenes in Food Safety

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Antibacterial effect of trans-cinnamaldehyde, eugenol, carvacrol, and thymol on Salmonella Enteritidis and Campylobacter jejuni in chicken cecal contents in vitro

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Kollanoor Johny, A., Darre, M.J., Donoghue, A.M., Donoghue, D.J., Venkitanarayanan, K.·2010·Journal of Applied Poultry Research
DOI 10.3382/japr.2010-00181
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Abstract

Salmonellaenterica serovar Enteritidis and Campylobacter jejuni are 2 major foodborne pathogens transmitted through poultry products. These pathogens colonize the chicken cecum, leading to contamination of carcasses during slaughter and subsequent processing operations. We investigated the antimicrobial efficacy of 4 GRAS (generally recognized as safe) status plant-derived molecules, namely, trans-cinnamaldehyde, eugenol, carvacrol, and thymol, against Salmonella Enteritidis and C. jejuni in chicken cecal contents in vitro. The plant molecules were added at different concentrations (ranging from 10 to 75 mM for Salmonella Enteritidis and 10 to 30 mM for C. jejuni) to autoclaved chicken cecal contents inoculated with approximately 7.0 log10 cfu/mL of Salmonella Enteritidis or approximately 5.0 log10 cfu/mL of C. jejuni. The pathogen populations in the cecal contents after 15 s, 8 h, and 24 h of incubation at 40°C were determined. Duplicate samples of treatments and the control were included, and the study was replicated 3 times. Campylobacter jejuni was more sensitive to all the molecules than Salmonella Enteritidis (P ≤ 0.05). All molecules were highly bactericidal, with the lowest concentration of trans-cinnamaldehyde (10 mM) reducing (P ≤ 0.05) Salmonella Enteritidis populations by approximately 6.0 log10 cfu/mL after 8 h and >8.0 log10 cfu/mL after 24 h of incubation. Trans-cinnamaldehyde at 25 mM eliminated detectable (P ≤ 0.05) Salmonella Enteritidis counts by 8 h of incubation. In addition, trans-cinnamaldehyde at all tested concentrations (10, 20, and 30 mM) reduced C. jejuni counts to <1.0 log10 cfu/mL after 8 and 24 h of incubation. Carvacrol and eugenol decreased (P ≤ 0.05) Salmonella Enteritidis and C. jejuni counts to <1.0 log10 cfu/mL at 50 and 75 mM and 20 and 30 mM, respectively. Although not as effective as other molecules, thymol also reduced the counts of both pathogens. We conclude that the aforementioned molecules could potentially be used to reduce Salmonella Enteritidis and C. jejuni in chicken ceca. Follow-up in vivo studies are planned to evaluate these possibilities.

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References 30 · Cited by 162

Research works printed but not linked: 27

  1. Heres, 2004, Effect of acidified feed on susceptibility of broiler chickens to intestinal infection by Campylobacter and Salmonella, Vet. Microbiol., 99, 259, 10.1016/j.vetmic.2003.12.008 · DOI
  2. White, 1997, Strategies to control Salmonella and Campylobacter in raw poultry products, Rev. Sci. Tech. Off. Int. Epiz., 16, 525, 10.20506/rst.16.2.1046 · DOI
  3. Centers for Disease Control and Prevention, 2005, Preliminary FoodNet data on the incidence of infection with pathogens commonly transmitted through food—10 sites, United States, 2004, Morb. Mort. Wkly. Rep., 54, 352
  4. Mead, 1999, Food-related illness and death in United States, Emerg. Infect. Dis., 5, 607, 10.3201/eid0505.990502 · DOI
  5. Stern, 2008, Salmonella species and Campylobacter jejuni. Cecal colonization model in broilers, Poult. Sci., 87, 2399, 10.3382/ps.2008-00140 · DOI
  6. Gantois, 2009, Mechanisms of egg contamination by Salmonella Enteritidis, FEMS Microbiol. Rev., 33, 718, 10.1111/j.1574-6976.2008.00161.x · DOI
  7. Okamura, 2001, Differences in abilities to colonize reproductive organs and to contaminate eggs in intravaginally inoculated hens and in vitro adherents to vaginal explants between Salmonella Enteritidis and other Salmonella serovars, Avian Dis., 45, 962, 10.2307/1592875 · DOI
  8. Salamci, 2007, Chemical compositions, antimicrobial and herbicidal effects of essential oils isolated from Turkish Tanacetum aucheranum and Tanacetum chiliophyllum var. chiliophyllum., Biochem. Syst. Ecol., 35, 569, 10.1016/j.bse.2007.03.012 · DOI
  9. Friedman, 2002, Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica., J. Food Prot., 65, 1545, 10.4315/0362-028X-65.10.1545 · DOI
  10. Kollanoor Johny, 2008, Antibacterial effect of trans-cinnamaldehyde on Salmonella Enteritidis and Campylobacter jejuni in chicken drinking water, J. Appl. Poult. Res., 17, 490, 10.3382/japr.2008-00051 · DOI
  11. Chun, 2005, Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori., Process Biochem., 40, 809, 10.1016/j.procbio.2004.02.018 · DOI
  12. Suhr, 2003, Antifungal activity of essential oils evaluated by two different application techniques against rye bread spoilage fungi, J. Appl. Microbiol., 94, 665, 10.1046/j.1365-2672.2003.01896.x · DOI
  13. Ananda Baskaran, 2009, Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro., J. Dairy Sci., 92, 1423, 10.3168/jds.2008-1384 · DOI
  14. Line, 2001, Development of selective differential agar for isolation and enumeration of Campylobacter spp, J. Food Prot., 64, 1711, 10.4315/0362-028X-64.11.1711 · DOI
  15. Vasudevan, 2005, In vitro inactivation of Salmonella Enteritidis in chicken cecal contents by caprylic acid, J. Appl. Poult. Res., 14, 122, 10.1093/japr/14.1.122 · DOI
  16. Djefal, 2007, Validation and substantiation of 25 kGy as sterilization dose for lyophilized human amnion membrane, Cell Tissue Bank., 8, 9, 10.1007/s10561-006-9016-x · DOI
  17. Kollanoor Johny, 2009, Prophylactic supplementation of caprylic acid in feed reduces Salmonella Enteritidis colonization in commercial broiler chicks, J. Food Prot., 72, 722, 10.4315/0362-028X-72.4.722 · DOI
  18. Solis de los Santos, 2008, Caprylic acid in feed reduces enteric Campylobacter jejuni colonization in ten-day-old broiler chickens, Poult. Sci., 87, 800, 10.3382/ps.2007-00280 · DOI
  19. Solis de los Santos, 2009, The natural feed additive caprylic acid decreases Campylobacter jejuni colonization in market-aged broiler chickens, Poult. Sci., 88, 61, 10.3382/ps.2008-00228 · DOI
  20. Corrier, 1990, Effect of dietary lactose on Salmonella colonization of market-age broiler chickens, Avian Dis., 34, 668, 10.2307/1591262 · DOI
  21. Stavric, 1992, Defined cultures and prospects, Int. J. Food Microbiol., 15, 245, 10.1016/0168-1605(92)90056-9 · DOI
  22. Solomon, 1999, Campylobacter jejuni: A bacterial paradox, J. Food Saf., 19, 121, 10.1111/j.1745-4565.1999.tb00239.x · DOI
  23. Si, 2006, Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria, J. Appl. Microbiol., 100, 296, 10.1111/j.1365-2672.2005.02789.x · DOI
  24. Ohno, 2003, Antimicrobial activity of essential oils against Helicobacter pylori., Helicobacter, 8, 207, 10.1046/j.1523-5378.2003.00146.x · DOI
  25. Domadia, 2007, Inhibition of bacterial cell division protein FtsZ by cinnamaldehyde, Biochem. Pharmacol., 832, 831, 10.1016/j.bcp.2007.06.029 · DOI · Paper page
  26. Sikkema, 1994, Interactions of cyclic hydrocarbons with biological membranes, J. Biol. Chem., 269, 8022, 10.1016/S0021-9258(17)37154-5 · DOI
  27. Ultee, 1999, Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus., Appl. Environ. Microbiol., 65, 4606, 10.1128/AEM.65.10.4606-4610.1999 · DOI · Paper page

Metadata

Authors
Kollanoor Johny, A., Darre, M. J., Donoghue, A. M., Donoghue, D. J., & Venkitanarayanan, K.
Affiliations
  • University of Connecticut (US)
  • Agricultural Research Service (US)
  • University of Arkansas at Fayetteville (US)
Corresponding author
Venkitanarayanan, K.
ORCID
Venue
Journal of Applied Poultry Research
Publisher
Elsevier BV
Published
2010-09
Volume
19
Issue
3
Pages
237-244
ISSN
1056-6171 (ISSN-L)
DOI
10.3382/japr.2010-00181
OpenAlex
W2144832779
Cited by
162
References
30
Citations by year
2026: 2 · 2025: 5 · 2024: 7 · 2023: 14 · 2022: 12 · 2021: 20 · 2020: 15 · 2019: 14 · 2018: 13 · 2017: 8 · 2016: 8 · 2015: 10 · 2014: 15 · 2013: 9 · 2012: 7
FWCI
9.32
Type
journal article
Language
en
Open access
Licence
Locations
The Journal of Applied Poultry Research (publishedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
Keywords
trans-cinnamaldehyde, eugenol, carvacrol, thymol, Salmonella Enteritidis, Campylobacter jejuni, antimicrobial efficacy, foodborne pathogens, poultry carcass contamination, plant-derived antimicrobials, GRAS compounds, bactericidal activity, poultry feed additives, food safety

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CoChat. (2026). Citation check: Antibacterial effect of trans-cinnamaldehyde, eugenol, carvacrol, and thymol on Salmonella Enteritidis and Campylobacter jejuni in chicken cecal contents in vitro. https://app.cochat.ai/papers/10.3382/japr.2010-00181
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Kollanoor Johny, A., Darre, M. J., Donoghue, A. M., Donoghue, D. J., & Venkitanarayanan, K. (2010). Antibacterial effect of trans-cinnamaldehyde, eugenol, carvacrol, and thymol on Salmonella Enteritidis and Campylobacter jejuni in chicken cecal contents in vitro. Journal of Applied Poultry Research. https://doi.org/10.3382/japr.2010-00181