Cited by 276Bacterial Genetics and BiotechnologyMicrotubule and mitosis dynamicsAntimicrobial agents and applications

Inhibition of bacterial cell division protein FtsZ by cinnamaldehyde

Domadia, Prerna, Swarup, Sanjay, Bhunia, Anirban, Sivaraman, J., Dasgupta, Debjani·2007·Biochemical Pharmacology

Abstract

Cinnamaldehyde is a natural product from spices that inhibits cell separation in Bacillus cereus. Cell division is regulated by FtsZ, a prokaryotic homolog of tubulin. FtsZ assembles into the Z-ring at the site of cell division. Here, we report the effect of cinnamaldehyde on FtsZ and hence on the cell division apparatus. Cinnamaldehyde decreases the in vitro assembly reaction and bundling of FtsZ. It is found that cinnamaldehyde perturbs the Z-ring morphology in vivo and reduces the frequency of the Z ring per unit cell length of Escherichia coli. In addition, GTP dependent FtsZ polymerization is inhibited by cinnamaldehyde. Cinnamaldehyde inhibits the rate of GTP hydrolysis and binds FtsZ with an affinity constant of 1.0+/-0.2 microM(-1). Isothermal titration calorimetry reveals that binding of cinnamaldehyde to FtsZ is driven by favorable enthalpic interactions. Further, we map the cinnamaldehyde binding region of FtsZ, using the saturation transfer difference-nuclear magnetic resonance and an in silico docking model. Both predict the cinnamaldehyde binding pocket at the C terminal region involving the T7 loop of FtsZ. Our results show that cinnamaldehyde binds FtsZ, perturbs the cytokinetic Z-ring formation and inhibits its assembly dynamics. This suggests that cinnamaldehyde, a small molecule of plant origin, is a potential lead compound that can be developed as an anti-FtsZ agent towards drug design.

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Crossref · 10.1016/j.bcp.2007.06.029

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55 of 55 references verified0 citation errors · 0 not checkable

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  • Cinnamaldehyde is capable of inhibiting cell separation in B. cereus.

    “Cinnamaldehyde, for example, is capable of inhibiting cell separation in B. cereus.”

    From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [79]

  • GTP-dependent FtsZ polymerisation is inhibited by cinnamaldehyde.

    “In addition, GTP-dependent FtsZ polymerisation is inhibited by cinnamaldehyde [79].”

    From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [79]

  • Cinnamaldehyde can decrease the in vitro assembly reaction and bundling of FtsZ.

    “cinnamaldehyde can decrease the in vitro assembly reaction and bundling of FtsZ.”

    From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [79]

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Authors
Domadia, P., Swarup, S., Bhunia, A., Sivaraman, J., & Dasgupta, D.
Affiliations
  • National University of Singapore (SG)
  • Nanyang Technological University (SG)
Corresponding author
  • Sivaraman, J.
  • Dasgupta, Debjani
Venue
Biochemical Pharmacology
Publisher
Elsevier BV
Published
2007-09
Volume
74
Issue
6
Pages
831-840
ISSN
0006-2952 (ISSN-L)
OpenAlex
W2029961182
Cited by
276
References
55
Citations by year
2026: 16 · 2025: 15 · 2024: 13 · 2023: 24 · 2022: 22 · 2021: 16 · 2020: 20 · 2019: 13 · 2018: 21 · 2017: 18 · 2016: 17 · 2015: 11 · 2014: 10 · 2013: 12 · 2012: 16
FWCI
4.11
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
Keywords
cinnamaldehyde, FtsZ, bacterial cell division, Z-ring, Escherichia coli, Bacillus cereus
MeSH terms
Acrolein, Anti-Bacterial Agents, Bacterial Proteins, Binding Sites, Cell Division, Cytoskeletal Proteins, Escherichia coli, Guanosine Triphosphate, Protein Binding, Recombinant Proteins

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