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Cited by 20Wound Healing and TreatmentsHydrogels: synthesis, properties, applicationsAntimicrobial agents and applications

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Carboxymethyl chitosan nanoparticle-modulated cationic hydrogels doped with copper ions for combating bacteria and facilitating wound healing

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Li, Yaqin, Lu, Jianping, Shi, Jingru, Zhang, Lingjiao, Mu, Haibo, Cui, Tong·2024·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2024.1429771PMC PMC11449867
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Abstract

The simultaneous administration of antibacterial treatment and acceleration of tissue regeneration are crucial for the effective healing of infected wounds. In this work, we developed a facile hydrogel (PCC hydrogel) through coordination and hydrogen interactions by polymerizing acrylamide monomers in the presence of carboxymethyl chitosan nanoparticles and copper ions. The prepared PCC hydrogel demonstrated effective bacterial capture from wound exudation and exhibited a potent bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa . Furthermore, slow release of copper ions from the hydrogel facilitated wound healing by promoting cell migration, collagen deposition and angiogenesis. Additionally, the PCC hydrogel possessed excellent biocompatibility and hemostatic properties. The practical effectiveness of PCC hydrogel in addressing bacterial infections and facilitating wound healing was verified using a mouse model of MRSA-induced wound infections. Overall, this work presents a simple yet efficient multifunctional hydrogel platform that integrates antibacterial activity, promotion of wound healing, and hemostasis for managing bacteria-associated wounds.

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Crossref · 10.3389/fbioe.2024.1429771

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SCHEME 1 Schematics for the preparation of PCC hydrogel, and its envisioned potential in bacterial capture, eradication and facilitation of wound healing.
SCHEME 1 Schematics for the preparation of PCC hydrogel, and its envisioned potential in bacterial capture, eradication and facilitation of wound healing.CC BY · PubMed Central
FIGURE 1 Characterization of PCC hydrogels. TEM image (A), DLS (B) and Zeta potential (C) of CMCS NPs. The rheological properties of PC (D) and PCC (E) hydrogels with various content of CMCS NPs by performing an oscillatory frequency sweep at a strain of 0.1%. (F) The SEM images of PAM, PC and PCC hydrogels. (G) The swelling ratio of the PC and PCC hydrogels. (n = 3). (H) XPS spectra of PCC hydrogel included a comprehensive survey of all elements and high-revolution spectra of Cu 2p, C 1s, N 1s. (I) EDS elemental mapping of Cu, C and N in PCC hydrogel. (J) FTIR spectra of PCC hydrogel and mixture of components in PCC hydrogel. The compressive stress-strain curves (K) and stretch change (L) of PCC hydrogel with various content of CMCS NPs. (M) The original, compression and recovery of PCC hydrogel.
FIGURE 1 Characterization of PCC hydrogels. TEM image (A), DLS (B) and Zeta potential (C) of CMCS NPs. The rheological properties of PC (D) and PCC (E) hydrogels with various content of CMCS NPs by performing an oscillatory frequency sweep at a strain of 0.1%. (F) The SEM images of PAM, PC and PCC hydrogels. (G) The swelling ratio of the PC and PCC hydrogels. (n = 3). (H) XPS spectra of PCC hydrogel included a comprehensive survey of all elements and high-revolution spectra of Cu 2p, C 1s, N 1s. (I) EDS elemental mapping of Cu, C and N in PCC hydrogel. (J) FTIR spectra of PCC hydrogel and mixture of components in PCC hydrogel. The compressive stress-strain curves (K) and stretch change (L) of PCC hydrogel with various content of CMCS NPs. (M) The original, compression and recovery of PCC hydrogel.CC BY · PubMed Central
FIGURE 2 Bacterial capture efficiency. OD600 (A) and relative SEM images (B) of bacteria suspensions incubated with PC or PC hydrogels for 10 min. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001.
FIGURE 2 Bacterial capture efficiency. OD600 (A) and relative SEM images (B) of bacteria suspensions incubated with PC or PC hydrogels for 10 min. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001.CC BY · PubMed Central
FIGURE 3 Antibacterial activity in vitro. Represent images of agar plates (A) and relative viability (B) of MRSA and PA following treatment with PC and PCC hydrogels. Photographs (C) and corresponding area percent (D) of inhibition zone. PCC treatment in MRSA or PA was normalized to 100% of inhibition zone. Fluorescence images (E) and SEM images (F) of bacteria treated with hydrogels. PI (red) indicated the dead bacteria and DAPI (blue) showed all bacterial cells. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, ***p < 0.001. ns, no significant difference.
FIGURE 3 Antibacterial activity in vitro. Represent images of agar plates (A) and relative viability (B) of MRSA and PA following treatment with PC and PCC hydrogels. Photographs (C) and corresponding area percent (D) of inhibition zone. PCC treatment in MRSA or PA was normalized to 100% of inhibition zone. Fluorescence images (E) and SEM images (F) of bacteria treated with hydrogels. PI (red) indicated the dead bacteria and DAPI (blue) showed all bacterial cells. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, ***p < 0.001. ns, no significant difference.CC BY · PubMed Central
FIGURE 4 (A) Cell viability of L929 cells following different treatment: PBS, Phenol and PCC extract medium diluted for different times. (B) Live/dead staining images of L929 cells after treated with PBS, PCC hydrogel and phenol. (C) Hemocompatibility of hydrogels. Data are presented as means and standard derivation (n = 3). (D) The release profile of Cu2+ from PCC hydrogel. (E) The effects of PC and PCC hydrogels on L929 cell migration. (F) Migration area. Data are presented as means and standard derivation (n = 6). Statistical significance is indicated by **p < 0.01, p < 0.001. ns, no significant difference.
FIGURE 4 (A) Cell viability of L929 cells following different treatment: PBS, Phenol and PCC extract medium diluted for different times. (B) Live/dead staining images of L929 cells after treated with PBS, PCC hydrogel and phenol. (C) Hemocompatibility of hydrogels. Data are presented as means and standard derivation (n = 3). (D) The release profile of Cu2+ from PCC hydrogel. (E) The effects of PC and PCC hydrogels on L929 cell migration. (F) Migration area. Data are presented as means and standard derivation (n = 6). Statistical significance is indicated by **p < 0.01, p < 0.001. ns, no significant difference.CC BY · PubMed Central
FIGURE 5 Hemostatic capacity of the hydrogels. (A) The hemostatic model was schematically depicted through the amputation of mice tails. The hemostatic time (B) and blood loss (C) of the model mice following treatment by gauze, PC and PCC hydrogels, and the group without any treatment was applied as a blank control. (D) BCI. (E) The represent images of different samples in whole blood clotting test. Left: blood cells incubated with water (blank), gauze, PC and PCC hydrogels. Right: the unattached blood cells dispersed by water following previous incubation. (F) The SEM images of hemocytes adhere to different samples. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001. ns indicated no significant difference.
FIGURE 5 Hemostatic capacity of the hydrogels. (A) The hemostatic model was schematically depicted through the amputation of mice tails. The hemostatic time (B) and blood loss (C) of the model mice following treatment by gauze, PC and PCC hydrogels, and the group without any treatment was applied as a blank control. (D) BCI. (E) The represent images of different samples in whole blood clotting test. Left: blood cells incubated with water (blank), gauze, PC and PCC hydrogels. Right: the unattached blood cells dispersed by water following previous incubation. (F) The SEM images of hemocytes adhere to different samples. Data are presented as means and standard derivation (n = 3). Statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001. ns indicated no significant difference.CC BY · PubMed Central
FIGURE 6 The therapeutic efficacy of hydrogel in MRSA-infected wound mice model. (A) Representative wound images. (B) The wound closure rates. (C) The H&E and Masson’s trichrome staining, and CD31/VEGF immunohistochemical staining of wound tissues following 7 days treatment. Scale bar: 100 µm. (D) The H&E staining of the major organs following 7 days treatment. Scale bar: 100 µm. Data are presented as means and standard derivation (n = 6). Statistical significance is indicated by *p < 0.05, **p < 0.01.
FIGURE 6 The therapeutic efficacy of hydrogel in MRSA-infected wound mice model. (A) Representative wound images. (B) The wound closure rates. (C) The H&E and Masson’s trichrome staining, and CD31/VEGF immunohistochemical staining of wound tissues following 7 days treatment. Scale bar: 100 µm. (D) The H&E staining of the major organs following 7 days treatment. Scale bar: 100 µm. Data are presented as means and standard derivation (n = 6). Statistical significance is indicated by *p < 0.05, **p < 0.01.CC BY · PubMed Central

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Metadata

Authors
Li, Y., Lu, J., Shi, J., Zhang, L., Mu, H., & Cui, T.
Affiliations
  • Northwest A&F University (CN)
  • Karamay Central Hospital (CN)
Corresponding author
  • Mu, Haibo
  • Cui, Tong
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2024-09-20
Volume
12
Pages
1429771-1429771
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2024.1429771
PMC
PMC11449867
OpenAlex
W4402693689
Cited by
19
References
42
Citations by year
2026: 13 · 2025: 6
FWCI
3.02
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Natural Science Foundation of Xinjiang
Topics
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
  • Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
Keywords
cationic hydrogels, copper doping, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, antibacterial wound dressing, infected wound healing, hemostasis, angiogenesis, collagen deposition, cell migration

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