CoChat
Sign Up
Cited by 84Tuberculosis Research and EpidemiologyMicroRNA in disease regulationImmune Response and Inflammation

Actin-binding protein regulation by microRNAs as a novel microbial strategy to modulate phagocytosis by host cells: the case of N-Wasp and miR-142-3p

Open access
Bettencourt, Paulo, Marion, Sabrina, Pires, David, Santos, Leonor F., Lastrucci, Claire, Carmo, Nuno, Blake, Jonathon, Benes, Vladimir +4 more·2013·Frontiers in Cellular and Infection Microbiology
DOI 10.3389/fcimb.2013.00019PMC PMC3672780
Open this paper in CoChat — free accountOpen PDF

Abstract

Mycobacterium tuberculosis (Mtb) is a successful intracellular pathogen that thrives in macrophages (Mφs). There is a need to better understand how Mtb alters cellular processes like phagolysosome biogenesis, a classical determinant of its pathogenesis. A central feature of this bacteria's strategy is the manipulation of Mφ actin. Here, we examined the role of microRNAs (miRNAs) as a potential mechanism in the regulation of actin-mediated events leading to phagocytosis in the context of mycobacteria infection. Given that non-virulent Mycobacterium smegmatis also controls actin filament assembly to prolong its intracellular survival inside host cells, we performed a global transcriptomic analysis to assess the modulation of miRNAs upon M. smegmatis infection of the murine Mφ cell line, J774A.1. This approach identified miR-142-3p as a key candidate to be involved in the regulation of actin dynamics required in phagocytosis. We unequivocally demonstrate that miR-142-3p targets N-Wasp, an actin-binding protein required during microbial challenge. A gain-of-function approach for miR-142-3p revealed a down-regulation of N-Wasp expression accompanied by a decrease of mycobacteria intake, while a loss-of-function approach yielded the reciprocal increase of the phagocytosis process. Equally important, we show Mtb induces the early expression of miR-142-3p and partially down-regulates N-Wasp protein levels in both the murine J774A.1 cell line and primary human Mφs. As proof of principle, the partial siRNA-mediated knock down of N-Wasp resulted in a decrease of Mtb intake by human Mφs, reflected in lower levels of colony-forming units (CFU) counts over time. We therefore propose the modulation of miRNAs as a novel strategy in mycobacterial infection to control factors involved in actin filament assembly and other early events of phagolysosome biogenesis.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3389/fcimb.2013.00019

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • miR-142-3p targets N-wasp, an actin-binding protein regulating actin dynamics during bacterial uptake.

    “miR-142–3p targets N-wasp, an actin-binding protein regulating actin dynamics during bacterial uptake [145,198].”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [145]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 71

  1. Andreansky S. Liu H. Turner S. McCullers J. A. Lang R. Rutschman R. (2005). WASP- mice exhibit defective immune responses to influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis BCG. Exp. Hematol. 33, 443– 451 10.1016/j.exphem.2004.12.006 15781335 · DOI
  2. Anes E. Kühnel M. P. Bos E. Moniz-Pereira J. Habermann A. Griffiths G. (2003). Selected lipids activate phagosome actin assembly and maturation resulting in killing of pathogenic mycobacteria. Nat. Cell Biol. 5, 793– 802 10.1038/ncb1036 12942085 · DOI
  3. Anes E. Peyron P. Staali L. Jordao L. Gutierrez M. G. Kress H. (2006). Dynamic life and death interactions between Mycobacterium smegmatis and J774 macrophages. Cell. Microbiol. 8, 939– 960 10.1111/j.1462-5822.2005.00675.x 16681836 · DOI
  4. Bellon M. Lepelletier Y. Hermine O. Nicot C. (2009). Deregulation of microRNA involved in hematopoiesis and the immune response in HTLV-I adult T-cell leukemia. Blood 113, 4914– 4917 10.1182/blood-2008-11-189845 19246560 PMC2686141 · DOI
  5. Betel D. Wilson M. Gabow A. Marks D. S. Sander C. (2008). The microRNA.org resource: targets and expression. Nucleic Acids Res. 36, D149– D153 10.1093/nar/gkm995 18158296 PMC2238905 · DOI
  6. Bissels U. Wild S. Tomiuk S. Hafner M. Scheel H. Mihailovic A. (2011). Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells. Stem Cells 29, 847– 857 10.1002/stem.627 21394831 PMC3116150 · DOI
  7. Boissonneault V. Plante I. Rivest S. Provost P. (2009). MicroRNA-298 and microRNA-328 regulate expression of mouse beta-amyloid precursor protein-converting enzyme 1. J. Biol. Chem. 284, 1971– 1981 10.1074/jbc.M807530200 18986979 PMC2908704 · DOI
  8. Careccia S. Mainardi S. Pelosi A. Gurtner A. Diverio D. Riccioni R. (2009). A restricted signature of miRNAs distinguishes APL blasts from normal promyelocytes. Oncogene 28, 4034– 4040 10.1038/onc.2009.255 19749800 · DOI
  9. Caron E. Crepin V. F. Simpson N. Knutton S. Garmendia J. Frankel G. (2006). Subversion of actin dynamics by EPEC and EHEC. Curr. Opin. Microbiol. 9, 40– 45 10.1016/j.mib.2005.12.008 16406772 · DOI
  10. Castandet J. Prost J. F. Peyron P. Astarie-Dequeker C. Anes E. Cozzone A. J. (2005). Tyrosine phosphatase MptpA of Mycobacterium tuberculosis inhibits phagocytosis and increases actin polymerization in macrophages. Res. Microbiol. 156, 1005– 1013 10.1016/j.resmic.2005.05.013 16085396 · DOI
  11. Cullen B. R. (2011). Viruses and microRNAs: RISCy interactions with serious consequences. Genes Dev. 25, 1881– 1894 10.1101/gad.17352611 21896651 PMC3185961 · DOI
  12. Dart A. E. Donnelly S. K. Holden D. W. Way M. Caron E. (2012). Nck and Cdc42 co-operate to recruit N-WASP to promote FcγR-mediated phagocytosis. J. Cell. Sci. 125, 2825– 2830 10.1242/jcs.106583 22454526 · DOI
  13. Deretic V. Vergne I. Chua J. Master S. Singh S. B. Fazio J. A. (2004). Endosomal membrane traffic: convergence point targeted by Mycobacterium tuberculosis and HIV. Cell. Microbiol. 6, 999– 1009 10.1111/j.1462-5822.2004.00449.x 15469429 · DOI
  14. Eulalio A. Schulte L. Vogel J. (2012b). The mammalian microRNA response to bacterial infections. RNA Biol. 9, 742– 750 10.4161/rna.20018 22664920 · DOI
  15. Frischknecht F. Moreau V. Röttger S. Gonfloni S. Reckmann I. Superti-Furga G. (1999). Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling. Nature 401, 926– 929 10.1038/44860 10553910 · DOI
  16. Fu Y. Yi Z. Wu X. Li J. Xu F. (2011). Circulating microRNAs in patients with active pulmonary tuberculosis. J. Clin. Microbiol. 49, 4246– 4251 10.1128/JCM.05459-11 21998423 PMC3232949 · DOI
  17. Gouin E. Gantelet H. Egile C. Lasa I. Ohayon H. Villiers V. (1999). A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J. Cell. Sci. 112(Pt 11), 1697– 1708 10318762
  18. Gutierrez M. G. Gonzalez A. P. Anes E. Griffiths G. (2009). Role of lipids in killing mycobacteria by macrophages: evidence for NF-kappaB-dependent and -independent killing induced by different lipids. Cell. Microbiol. 11, 406– 420 10.1111/j.1462-5822.2008.01263.x 19016780 · DOI
  19. Hakimi M. A. Cannella D. (2011). Apicomplexan parasites and subversion of the host cell microRNA pathway. Trends Parasitol. 27, 481– 486 10.1016/j.pt.2011.07.001 21840260 · DOI
  20. Huang B. Zhao J. Lei Z. Shen S. Li D. Shen G. X. (2009). miR-142-3p restricts cAMP production in CD4+CD25− T cells and CD4+CD25+ TREG cells by targeting AC9 mRNA. EMBO Rep. 10, 180– 185 10.1038/embor.2008.224 19098714 PMC2637310 · DOI
  21. Impey S. Davare M. Lasiek A. Fortin D. Ando H. Varlamova O. (2010). An activity-induced microRNA controls dendritic spine formation by regulating Rac1-PAK signaling. Mol. Cell. Neurosci. 43, 146– 156 10.1016/j.mcn.2009.10.005 19850129 PMC2818337 · DOI · Paper page
  22. Jordao L. Bleck C. K. Mayorga L. Griffiths G. Anes E. (2008a). On the killing of mycobacteria by macrophages. Cell. Microbiol. 10, 529– 548 10.1111/j.1462-5822.2007.01067.x 17986264 · DOI
  23. Jordao L. Lengeling A. Bordat Y. Boudou F. Gicquel B. Neyrolles O. (2008b). Effects of omega-3 and -6 fatty acids on Mycobacterium tuberculosis in macrophages and in mice. Microbes Infect. 10, 1379– 1386 10.1016/j.micinf.2008.08.004 18771745 · DOI
  24. Kalamidas S. A. Kuehnel M. P. Peyron P. Rybin V. Rauch S. Kotoulas O. B. (2006). cAMP synthesis and degradation by phagosomes regulate actin assembly and fusion events: consequences for mycobacteria. J. Cell. Sci. 119, 3686– 3694 10.1242/jcs.03091 16931599 · DOI
  25. Kalman D. Weiner O. D. Goosney D. L. Sedat J. W. Finlay B. B. Abo A. (1999). Enteropathogenic, E. coli acts through WASP and Arp2/3 complex to form actin pedestals. Nat. Cell Biol. 1, 389– 391 10.1038/14087 10559969 PMC2828053 · DOI
  26. Kano M. Seki N. Kikkawa N. Fujimura L. Hoshino I. Akutsu Y. (2010). miR-145, miR-133a and miR-133b: tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int. J. Cancer 127, 2804– 2814 10.1002/ijc.25284 21351259 · DOI
  27. Kjeken R. Egeberg M. Habermann A. Kuehnel M. Peyron P. Floetenmeyer M. (2004). Fusion between phagosomes, early and late endosomes: a role for actin in fusion between late, but not early endocytic organelles. Mol. Biol. Cell 15, 345– 358 10.1091/mbc.E03-05-0334 14617814 PMC307552 · DOI
  28. Kozomara A. Griffiths-Jones S. (2011). miRBase: integrating microRNA annotation and deep-sequencing data. Nucleic Acids Res. 39, D152– D157 10.1093/nar/gkq1027 21037258 PMC3013655 · DOI
  29. Krek A. Grün D. Poy M. N. Wolf R. Rosenberg L. Epstein E. J. (2005). Combinatorial microRNA target predictions. Nat. Genet. 37, 495– 500 10.1038/ng1536 15806104 · DOI · Paper page
  30. Kuehnel M. P. Rybin V. Anand P. K. Anes E. Griffiths G. (2009). Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen. J. Cell. Sci. 122, 499– 504 10.1242/jcs.034199 19174471 · DOI
  31. Kühnel M. Mayorga L. S. Dandekar T. Thakar J. Schwarz R. Anes E. (2008). Modelling phagosomal lipid networks that regulate actin assembly. BMC Syst. Biol. 2: 107 10.1186/1752-0509-2-107 19061496 PMC2628873 · DOI
  32. Lefèvre L. Lugo-Villarino G. Meunier E. Valentin A. Olagnier D. Authier H. (2013). The C-type Lectin Receptors Dectin-1, MR, and SIGNR3 Contribute Both Positively and Negatively to the Macrophage Response to Leishmania infantum. Immunity 38, 1038– 1049 10.1016/j.immuni.2013.04.010 23684988 · DOI
  33. Lewis B. P. Burge C. B. Bartel D. P. (2005). Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120, 15– 20 10.1016/j.cell.2004.12.035 15652477 · DOI · Paper page
  34. Liu H. Cao Y. D. Ye W. X. Sun Y. Y. (2010). Effect of microRNA-206 on cytoskeleton remodelling by downregulating Cdc42 in MDA-MB-231 cells. Tumori 96, 751– 755 21302623
  35. Ma F. Xu S. Liu X. Zhang Q. Xu X. Liu M. (2011). The microRNA miR-29 controls innate and adaptive immune responses to intracellular bacterial infection by targeting interferon-γ. Nat. Immunol. 12, 861– 869 10.1038/ni.2073 21785411 · DOI
  36. Maragkakis M. Alexiou P. Papadopoulos G. L. Reczko M. Dalamagas T. Giannopoulos G. (2009a). Accurate microRNA target prediction correlates with protein repression levels. BMC Bioinformatics 10: 295 10.1186/1471-2105-10-295 19765283 PMC2752464 · DOI
  37. Maragkakis M. Reczko M. Simossis V. A. Alexiou P. Papadopoulos G. L. Dalamagas T. (2009b). DIANA-microT web server: elucidating microRNA functions through target prediction. Nucleic Acids Res. 37, W273– W276 10.1093/nar/gkp292 19406924 PMC2703977 · DOI
  38. McGee K. Zettl M. Way M. Fällman M. (2001). A role for N-WASP in invasin-promoted internalisation. FEBS Lett. 509, 59– 65 10.1016/S0014-5793(01)03139-8 11734206 · DOI
  39. O'Connell R. M. Rao D. S. Baltimore D. (2012). microRNA regulation of inflammatory responses. Annu. Rev. Immunol. 30, 295– 312 10.1146/annurev-immunol-020711-075013 22224773 · DOI
  40. Park H. Cox D. (2009). Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP. Mol. Biol. Cell 20, 4500– 4508 10.1091/mbc.E09-03-0230 19741094 PMC2770938 · DOI
  41. Park S. Y. Lee J. H. Ha M. Nam J. W. Kim V. N. (2009). miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Nat. Struct. Mol. Biol. 16, 23– 29 10.1038/nsmb.1533 19079265 · DOI
  42. Qi Y. Cui L. Ge Y. Shi Z. Zhao K. Guo X. (2012). Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection. BMC Infect. Dis. 12: 384 10.1186/1471-2334-12-384 23272999 PMC3568404 · DOI
  43. Saeed A. I. Bhagabati N. K. Braisted J. C. Liang W. Sharov V. Howe E. A. (2006). TM4 microarray software suite. Methods Enzymol. 411, 134– 193 10.1016/S0076-6879(06)11009-5 16939790 · DOI
  44. Sharbati J. Lewin A. Kutz-Lohroff B. Kamal E. Einspanier R. Sharbati S. (2011). Integrated microRNA-mRNA-analysis of human monocyte derived macrophages upon Mycobacterium avium subsp. hominissuis infection. PLoS ONE 6: e20258 10.1371/journal.pone.0020258 21629653 PMC3101234 · DOI
  45. Singh Y. Kaul V. Mehra A. Chatterjee S. Tousif S. Dwivedi V. P. (2013). Mycobacterium tuberculosis controls microRNA-99b (miR-99b) expression in infected murine dendritic cells to modulate host immunity. J. Biol. Chem. 288, 5056– 5061 10.1074/jbc.C112.439778 23233675 PMC3576108 · DOI
  46. Sossey-Alaoui K. Bialkowska K. Plow E. F. (2009). The miR200 family of microRNAs regulates WAVE3-dependent cancer cell invasion. J. Biol. Chem. 284, 33019– 33029 10.1074/jbc.M109.034553 19801681 PMC2785142 · DOI
  47. Spinelli S. V. Diaz A. D'Attilio L. Marchesini M. M. Bogue C. Bay M. L. (2013). Altered microRNA expression levels in mononuclear cells of patients with pulmonary and pleural tuberculosis and their relation with components of the immune response. Mol. Immunol. 53, 265– 269 10.1016/j.molimm.2012.08.008 22964481 · DOI
  48. Stamm L. M. Morisaki J. H. Gao L. Y. Jeng R. L. McDonald K. L. Roth R. (2003). Mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility. J. Exp. Med. 198, 1361– 1368 10.1084/jem.20031072 14597736 PMC2194249 · DOI
  49. Stamm L. M. Pak M. A. Morisaki J. H. Snapper S. B. Rottner K. Lommel S. (2005). Role of the WASP family proteins for Mycobacterium marinum actin tail formation. Proc. Natl. Acad. Sci. U.S.A. 102, 14837– 14842 10.1073/pnas.0504663102 16199520 PMC1239894 · DOI
  50. Sun Y. Varambally S. Maher C. A. Cao Q. Chockley P. Toubai T. (2011). Targeting of microRNA-142-3p in dendritic cells regulates endotoxin-induced mortality. Blood 117, 6172– 6183 10.1182/blood-2010-12-325647 21474672 PMC3122940 · DOI
  51. Suzuki T. Miki H. Takenawa T. Sasakawa C. (1998). Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri. EMBO J. 17, 2767– 2776 10.1093/emboj/17.10.2767 9582270 PMC1170617 · DOI
  52. Tailleux L. Neyrolles O. Honoré-Bouakline S. Perret E. Sanchez F. Abastado J. P. (2003). Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells. J. Immunol. 170, 1939– 1948 12574362
  53. Treede I. Braun A. Sparla R. Kühnel M. Giese T. Turner J. R. (2007). Anti-inflammatory effects of phosphatidylcholine. J. Biol. Chem. 282, 27155– 27164 10.1074/jbc.M704408200 17636253 PMC2693065 · DOI
  54. Tusher V. G. Tibshirani R. Chu G. (2001). Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. U.S.A. 98, 5116– 5121 10.1073/pnas.091062498 11309499 PMC33173 · DOI · Paper page
  55. Unsworth K. E. Way M. McNiven M. Machesky L. Holden D. W. (2004). Analysis of the mechanisms of Salmonella-induced actin assembly during invasion of host cells and intracellular replication. Cell. Microbiol. 6, 1041– 1055 10.1111/j.1462-5822.2004.00417.x 15469433 · DOI
  56. Voinnet O. (2011). Micro-balancing innate immunity to Salmonella. EMBO J. 30, 1877– 1879 10.1038/emboj.2011.134 21593727 PMC3098492 · DOI
  57. Wang C. Peyron P. Mestre O. Kaplan G. Van Soolingen D. Gao Q. (2010). Innate immune response to Mycobacterium tuberculosis Beijing and other genotypes. PLoS ONE 5: e13594 10.1371/journal.pone.0013594 21049036 PMC2963601 · DOI
  58. Wang C. Yang S. Sun G. Tang X. Lu S. Neyrolles O. (2011). Comparative miRNA expression profiles in individuals with latent and active tuberculosis. PLoS ONE 6: e25832 10.1371/journal.pone.0025832 22003408 PMC3189221 · DOI
  59. Wang H. Garzon R. Sun H. Ladner K. J. Singh R. Dahlman J. (2008). NF-kappaB-YY1-miR-29 regulatory circuitry in skeletal myogenesis and rhabdomyosarcoma. Cancer Cell 14, 369– 381 10.1016/j.ccr.2008.10.006 18977326 PMC3829205 · DOI
  60. Wang X. (2008). miRDB: a microRNA target prediction and functional annotation database with a wiki interface. RNA 14, 1012– 1017 10.1261/rna.965408 18426918 PMC2390791 · DOI
  61. Wang X. El Naqa I. M. (2008). Prediction of both conserved and nonconserved microRNA targets in animals. Bioinformatics 24, 325– 332 10.1093/bioinformatics/btm595 18048393 · DOI
  62. Wang X. S. Gong J. N. Yu J. Wang F. Zhang X. H. Yin X. L. (2012). MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119, 4992– 5004 10.1182/blood-2011-10-385716 22493297 · DOI
  63. Williams A. E. (2008). Functional aspects of animal microRNAs. Cell. Mol. Life Sci. 65, 545– 562 10.1007/s00018-007-7355-9 17965831 PMC11131689 · DOI
  64. Wu L. Cai C. Wang X. Liu M. Li X. Tang H. (2011). MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells. FEBS Lett. 585, 1322– 1330 10.1016/j.febslet.2011.03.067 21482222 · DOI
  65. Wu Z. Lu H. Sheng J. Li L. (2012). Inductive microRNA-21 impairs anti-mycobacterial responses by targeting IL-12 and Bcl-2. FEBS Lett. 586, 2459– 2467 10.1016/j.febslet.2012.06.004 22710123 · DOI
  66. Wu H. Ye C. Ramirez D. Manjunath N. (2009). Alternative processing of primary microRNA transcripts by Drosha generates 5' end variation of mature microRNA. PLoS ONE 4: e7566 10.1371/journal.pone.0007566 19859542 PMC2762519 · DOI
  67. Xin M. Small E. M. Sutherland L. B. Qi X. McAnally J. Plato C. F. (2009). MicroRNAs miR-143 and miR-145 modulate cytoskeletal dynamics and responsiveness of smooth muscle cells to injury. Genes Dev. 23, 2166– 2178 10.1101/gad.1842409 19720868 PMC2751981 · DOI
  68. Xiong Y. Fang J. H. Yun J. P. Yang J. Zhang Y. Jia W. H. (2010). Effects of microRNA-29 on apoptosis, tumorigenicity, and prognosis of hepatocellular carcinoma. Hepatology 51, 836– 845 10.1002/hep.23380 20041405 · DOI
  69. Yi Z. Fu Y. Ji R. Li R. Guan Z. (2012). Altered microRNA signatures in sputum of patients with active pulmonary tuberculosis. PLoS ONE 7: e43184 10.1371/journal.pone.0043184 22900099 PMC3416796 · DOI
  70. Yu J. Peng H. Ruan Q. Fatima A. Getsios S. Lavker R. M. (2010). MicroRNA-205 promotes keratinocyte migration via the lipid phosphatase SHIP2. FASEB J. 24, 3950– 3959 10.1096/fj.10-157404 20530248 PMC2996908 · DOI
  71. Zhu S. Si M. L. Wu H. Mo Y. Y. (2007). MicroRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1). J. Biol. Chem. 282, 14328– 14336 10.1074/jbc.M611393200 17363372 · DOI · Paper page

Cited by

Sorted by citations

Authors
Bettencourt, P., Marion, S., Pires, D., Santos, L. F., Lastrucci, C., Carmo, N., Blake, J., Benes, V., Griffiths, G., Neyrolles, O., Lugo-Villarino, G., & Anes, E.
Affiliations
  • University of Lisbon (PT)
  • Centre National de la Recherche Scientifique (FR)
  • European Molecular Biology Laboratory (DE)
  • University of Oslo (NO)
Corresponding author
Anes, Elsa
ORCID
Venue
Frontiers in Cellular and Infection Microbiology
Publisher
Frontiers Media SA
Published
2013
Volume
3
Pages
19-19
ISSN
2235-2988 (ISSN-L)
DOI
10.3389/fcimb.2013.00019
PMC
PMC3672780
OpenAlex
W2027751052
Cited by
84
References
4
Citations by year
2026: 5 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 8 · 2021: 15 · 2020: 10 · 2019: 7 · 2018: 8 · 2017: 7 · 2016: 6 · 2015: 2 · 2014: 4 · 2013: 2
FWCI
3.40
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Cellular and Infection Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • London School of Hygiene and Tropical Medicine
  • Fondation pour la Recherche Médicale
  • Centre National de la Recherche Scientifique
  • Fundação para a Ciência e a Tecnologia (PTDC/BIA-BCM/102123/2008)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
Mycobacterium tuberculosis, Mycobacterium smegmatis, microRNA, miR-142-3p, N-WASP, actin-binding proteins, phagocytosis, macrophages, phagolysosome biogenesis, actin filament assembly, host-pathogen interaction, bacterial virulence, J774A.1 macrophages
MeSH terms
Animals, Cells, Cultured, Humans, Mycobacterium tuberculosis, Phagocytosis, Mycobacterium smegmatis, Gene Expression Profiling, MicroRNAs, Wiskott-Aldrich Syndrome Protein, Neuronal, Mice, Host-Pathogen Interactions

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review