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Cited by 15Gynecological conditions and treatmentsPeriodontal Regeneration and TreatmentsTissue Engineering and Regenerative Medicine

Platelet-rich fibrin improves repair and regeneration of damaged endometrium in rats

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Mao, Lele, Wang, XiaoXue, Sun, Yu, Yang, Mukun, Chen, Xing, Cui, Lei, Bai, Wenpei·2023·Frontiers in Endocrinology
DOI 10.3389/fendo.2023.1154958PMC PMC10443704
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Abstract

Purpose: Intrauterine adhesion (IUA) is the most common cause of uterine infertility. This study aims to evaluate whether platelet-rich fibrin (PRF) treatment can stimulate damaged endometrium regeneration in rats. Methods: First, hematoxylin and eosin (HE) staining, scanning and transmission electron microscopy, and ELISAs were used to evaluate the microstructure of PRF. Then, mechanical damage was used to establish an IUA rat model. A total of 40 SD female rats were randomized to three groups: PRF transplantation group, IUA group, and sham group. Rats were sacrificed at 3, 7, and 14 days and uteruses were obtained for further analysis. Finally, functional and histological recovery of the damaged endometrium was analyzed by pregnancy test, HE staining, Masson’s staining, and immunohistochemistry. Results: PRF has two distinct zones, platelets and fibrin zone. Long and narrow fibrin fibers interconnected with each other and formed a three-dimensional, flexible, and elastic structure; platelet aggregates were trapped in fibrin fibers, and each platelet is associated with several fibrin fibers. PRF exudates promoted endometrial stromal cell proliferation and migration in vitro. PRF transplantation was beneficial for maintaining uterine structure, promoting endometrial luminal epithelium and endometrial gland regeneration, and decreasing fibrotic areas in vivo. Conclusion: Intrauterine administration of PRF was demonstrated to be effective in preventing IUA and stimulating damaged endometrium regeneration in rats. This study not only provided a promising method for its potential in endometrial regeneration in women who suffer from uterine infertility but also may prevent IUA after intrauterine surgery in clinical cases.

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  • PRF-treated rats showed a significant increase in fertility, with pregnancy rates increasing from 16.7% in the untreated IUA group to 75% in the PRF-treated group.

    “In addition to other improvements, the rats treated with PRF also showed a significant increase in fertility, as evidenced by the increase in pregnancy rates from 16.7% in the untreated IUA group to 75% in the PRF-treated group.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [68]

    The Results (Evaluation of regenerative endometrium, Figure 6G) report a higher pregnancy rate in PRF-treated rats than in untreated IUA rats, and the figure caption includes significance markers. The reported rates, however, are 44.44% (4/9) and 10% (1/10), not 75% and 16.7%. (LLM, conf=0.89)

    Experimental — accuracy not yet measured

  • PRF is a valuable autologous therapeutic approach for the prevention of iatrogenic uterine perforation (IUA) and the subsequent restoration of endometrial tissue integrity.

    “Further immunohistochemical analysis confirmed the enhanced proliferation and epithelial recovery observed, thus bolstering the promise of platelet-rich fibrin (PRF) as a valuable autologous therapeutic approach for the prevention of iatrogenic uterine perforation (IUA) and the subsequent restoration of endometrial tissue integrity, as evidenced by the findings presented in the study (Figure 3(D1–”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [68]

  • In a 2023 rat model of intrauterine adhesion, platelet-rich fibrin (PRF) transplantation resulted in a significant improvement in endometrial regeneration while concurrently decreasing the fibrotic area from approximately 25% to approximately 6%.

    “A 2023 study conducted by Mao et al. using a rat model of intrauterine adhesion demonstrated that platelet-rich fibrin (PRF) transplantation resulted in a significant improvement in endometrial regeneration while concurrently decreasing the fibrotic area from approximately 25% to approximately 6%.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [68]

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Cited by

Sorted by citations

Authors
Mao, L., Wang, X., Sun, Y., Yang, M., Chen, X., Cui, L., & Bai, W.
Affiliations
  • Peking University (CN)
  • Capital Medical University (CN)
Corresponding author
  • Cui, Lei
  • Bai, Wenpei
ORCID
Venue
Frontiers in Endocrinology
Publisher
Frontiers Media SA
Published
2023-08-08
Volume
14
Pages
1154958-1154958
ISSN
1664-2392 (ISSN-L)
DOI
10.3389/fendo.2023.1154958
PMC
PMC10443704
OpenAlex
W4385666073
Cited by
15
References
2
Citations by year
2026: 5 · 2025: 5 · 2024: 4 · 2023: 1
FWCI
4.25
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Endocrinology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Natural Science Foundation of Beijing Municipality
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
platelet-rich fibrin, intrauterine adhesions, endometrial regeneration, endometrial repair, uterine factor infertility, endometrial fibrosis, platelet aggregates, rat model
MeSH terms
Platelet-Rich Fibrin, Animals, Endometrium, Eosine Yellowish-(YS), Female, Fibrin, Infertility, Pregnancy, Regeneration, Rats

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