Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle
Open accessAbstract
Objective To determine whether menstrual cycle stage or activity has an effect on the clonogenic activity of human endometrial epithelial and stromal cells.Design Clonal analysis of human endometrial epithelial and stromal cells derived from full-thickness endometrium.Setting University research laboratory.Patient(s) Twenty-six women of varying age and race undergoing hysterectomy for nonendometrial pathologies.Intervention(s) Full-thickness human endometrial tissue was dissociated into single cells. Epithelial and stromal cells were separated using magnetic beads, and cloning assays were performed in serum-containing or growth factor-supplemented serum-free medium.Main outcome measure(s) Clonogenic activity of epithelial and stromal cells.Result(s) Clonogenicity of epithelial and stromal cells did not vary significantly between proliferative, secretory, and inactive endometrium. However, epithelial and stromal cells did show a trend for greater numbers of clonogenic cells in secretory and proliferative endometrium respectively. A large variation between samples was observed, which may have masked any significant differences.Conclusion(s) We found that clonogenicity does not vary from the proliferative to secretory stage of the menstrual cycle, or between active, cycling and inactive endometrium for both epithelial and stromal cells. We have demonstrated for the first time that inactive endometrium contains clonogenic epithelial and stromal cells.
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Rare stromal and epithelial cells (1.25 and 0.22%, respectively) with high proliferative capacity were identified, providing the first evidence of endometrial stem cells.
“Rare stromal and epithelial cells (1.25 and 0.22%, respectively) with high proliferative capacity were identified, providing the first evidence of endometrial stem cells (Chan et al., 2004;Schwab et al., 2005).”
From Endometriosis and nuclear receptors (2019) · cites this paper as Schwab et al., 2005
The introductory passage labelled Abstract reports the stated percentages for clonogenic stromal and epithelial cells, but says only the relatively few cells forming large colonies had high proliferative potential; the percentages also include small colonies with low proliferative potential. The Discussion describes these cells as evidence supporting a putative endometrial stem-cell niche, not definitive identification of stem cells. (LLM, conf=0.85)
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Self-citation
- Authors
- Schwab, K. E., Chan, R. W. S., & Gargett, C. E.
- Affiliations
- Monash Medical Centre (AU)
- Corresponding author
- Gargett, Caroline Eve
- Venue
- Fertility and Sterility
- Publisher
- Elsevier BV
- Published
- 2005-10
- Volume
- 84
- Pages
- 1124-1130
- ISSN
- 0015-0282 (ISSN-L)
- OpenAlex
- W2106686929
- Cited by
- 249
- References
- 35
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 6 · 2023: 5 · 2022: 19 · 2021: 12 · 2020: 8 · 2019: 12 · 2018: 13 · 2017: 21 · 2016: 12 · 2015: 13 · 2014: 23 · 2013: 20 · 2012: 16
- FWCI
- 3.87
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Fertility and Sterility (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Monash University
- Medical Research Council
- National Health and Medical Research Council (124331, 284344)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- endometrial stem cells, endometrial epithelial cells, endometrial stromal cells, clonogenic activity, menstrual cycle, proliferative phase, luteal phase, endometrium, clonal analysis
- MeSH terms
- Adult, Cell Differentiation, Cells, Cultured, Clone Cells, Endometrium, Epithelial Cells, Female, Humans, Menstrual Cycle, Middle Aged, Stem Cells, Stromal Cells
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