High-Resolution Melting (HRM) Analysis Reveals Genotypic Differentiation of Venturia inaequalis Populations in Greece
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During the recent years, High Resolution Melting (HRM) analysis has been developed as a rapid and accurate tool in plant disease diagnostics, species identification and SNP genotyping. This approach has been applied to analyze the genetic diversity in several plant species with molecular markers, including single sequence repeats (SSR). However, no studies have been carried out to investigate the variation of SSR in plant pathogenic fungi by using the HRM technology. In this report, the genetic structure of Venturia inaequalis populations in Greece was investigated for the first time by using six microsatellite markers. The developed HRM protocol was able to generate genotype-specific melting curves, consistent with the haploid nature of the fungus. Compared to the more complexed genome of diploid plants, several limitations were avoided. The shape of the melting curves revealed differences between the genotypes in each SSR marker and showed that all the genotypes could be easily distinguished. The genetic analysis of apple scab populations revealed high genetic variation within the populations (96%), while only 4% of the total gene diversity was attributed to among-population variation. The isolates were grouped into three populations according to the principal coordinate analysis (PCoA).
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HRM generates sequence-related melting profiles and reveals the genotype at the level of a single nucleotide.
“It generates sequence-related melting profiles and reveals the genotype at the level of a single nucleotide (Chatzidimopoulos et al., 2019).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Chatzidimopoulos et al., 2019
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Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“It generates sequence-related melting profiles and reveals the genotype at the level of a single nucleotide (Chatzidimopoulos et al., 2019).”
- Authors
- Chatzidimopoulos, M., Ganopoulos, I., Moraitou-Daponta, E., Lioliopoulou, F., Ntantali, O., Panagiotaki, P., & Vellios, E. K.
- Affiliations
- University of Thessaly (GR)
- Corresponding author
- Chatzidimopoulos, Michael
- ORCID
- Venue
- Frontiers in Ecology and Evolution
- Publisher
- Frontiers Media SA
- Published
- 2019-12-13
- Volume
- 7
- ISSN
- 2296-701X (ISSN-L)
- OpenAlex
- W2997692950
- Cited by
- 19
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 7 · 2022: 4 · 2021: 1 · 2020: 2
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Ecology and Evolution (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- University of Thessaly Institutional Repository (University of Thessaly) (submittedVersion)
- Funders
- State Scholarships Foundation (2014-2020, NSRF 2014-2020)
- European Social Fund (20142020)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
- Keywords
- Venturia inaequalis, apple scab, high-resolution melting analysis, microsatellite markers, genetic diversity, population structure, plant pathogenic fungi, genotype differentiation, Greece
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