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Addressing Bias in Small RNA Library Preparation for Sequencing: A New Protocol Recovers MicroRNAs that Evade Capture by Current Methods

Baran-Gale, Jeanette, Kurtz, C. Lisa, Erdos, Michael R., Sison, Christina, Young, Alice, Fannin, Emily E., Chines, Peter S., Sethupathy, Praveen·2015·Frontiers in Genetics
DOI 10.3389/fgene.2015.00352PMC PMC4686641

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  • The main drivers of this difference were the changing methods of the Illumina sequencing library kits acting upon a small subset of microRNAs.

    “We determined that the main drivers of this difference were the changing methods of the Illumina sequencing library kits acting upon a small subset of microRNAs (Supplemental Fig. S10A; Baran-Gale et al. 2015).”

    From Toward the human cellular microRNAome (2017) · cites this paper as Baran-Gale et al. 2015

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  16. Poy M. N. Eliasson L. Krutzfeldt J. Kuwajima S. Ma X. MacDonald P. E. (2004). A pancreatic islet-specific microRNA regulates insulin secretion. Nature 432 226– 230. 10.1038/nature03076 15538371 · DOI · Paper page
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  27. Zhuang F. Fuchs R. T. Sun Z. Zheng Y. Robb G. B. (2012). Structural bias in T4 RNA ligase-mediated 3’-adapter ligation. Nucleic Acids Res. 40: e54 10.1093/nar/gkr1263 PMC3326334 22241775 · DOI

Cited by

Sorted by citations

Authors
Baran-Gale, J., Kurtz, C. L., Erdos, M. R., Sison, C., Young, A., Fannin, E. E., Chines, P. S., & Sethupathy, P.
Venue
Frontiers in Genetics
Publisher
Frontiers Media SA
Published
2015-12-22
Volume
6
ISSN
1664-8021
DOI
10.3389/fgene.2015.00352
PMC
PMC4686641
Cited by
98
References
2
Funders
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK091318)
  • National Human Genome Research Institute
  • University of North Carolina

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