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Endotoxin Contamination in Nanomaterials Leads to the Misinterpretation of Immunosafety Results

Li, Yang, Fujita, Mayumi, Boraschi, Diana·2017·Frontiers in Immunology
DOI 10.3389/fimmu.2017.00472PMC PMC5420554
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Figure 1 Endotoxin contamination in nanoparticles (NPs) induces inflammatory effects. (A) Endotoxin contamination in different nanomaterials evaluated by Limulus amebocyte lysate assay. (B) Gold (Au) NPs were deliberately contaminated with 1 µg/ml lipopolysaccharide (LPS) for 1 h at room temperature and then thoroughly washed with endotoxin-free water to eliminate unbound LPS. Human primary monocytes were exposed to either endotoxin-free or endotoxin-coated Au NPs for 24 h. The production of interleukin (IL)-1β in the culture supernatants was measured by ELISA [data partially presented in the supporting material of Ref. (30)].
Figure 1 Endotoxin contamination in nanoparticles (NPs) induces inflammatory effects. (A) Endotoxin contamination in different nanomaterials evaluated by Limulus amebocyte lysate assay. (B) Gold (Au) NPs were deliberately contaminated with 1 µg/ml lipopolysaccharide (LPS) for 1 h at room temperature and then thoroughly washed with endotoxin-free water to eliminate unbound LPS. Human primary monocytes were exposed to either endotoxin-free or endotoxin-coated Au NPs for 24 h. The production of interleukin (IL)-1β in the culture supernatants was measured by ELISA [data partially presented in the supporting material of Ref. (30)].CC BY · PubMed Central

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Research works printed but not linked: 46

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Metadata

Authors
Li, Y., Fujita, M., & Boraschi, D.
Venue
Frontiers in Immunology
Publisher
Frontiers Media SA
Published
2017-05-08
Volume
8
ISSN
1664-3224
DOI
10.3389/fimmu.2017.00472
PMC
PMC5420554
Cited by
99
References
1

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