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Cited by 31Cell Adhesion Molecules ResearchT-cell and B-cell ImmunologyProtein Kinase Regulation and GTPase Signaling

Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells

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Waldt, Natalie, Seifert, Anke, Demiray, Yunus Emre, Devroe, Eric, Turk, Benjamin E., Reichardt, Peter, Mix, Charlie, Reinhold, Annegret +5 more·2018·Frontiers in Immunology
DOI 10.3389/fimmu.2018.02852PMC PMC6290345
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Abstract

The integrin LFA-1 (CD11a/CD18) plays a critical role in the interaction of T cells with antigen presenting cells (APCs) to promote lymphocyte differentiation and proliferation. This integrin can be present either in a closed or in an open active conformation and its activation upon T-cell receptor (TCR) stimulation is a critical step to allow interaction with APCs. In this study we demonstrate that the serine/threonine kinase Ndr2 is critically involved in the initiation of TCR-mediated LFA-1 activation (open conformation) in T cells. Ndr2 itself becomes activated upon TCR stimulation and phosphorylates the intracellular integrin binding partner Filamin A (FLNa) at serine 2152. This phosphorylation promotes the dissociation of FLNa from LFA-1, allowing for a subsequent association of Talin and Kindlin-3 which both stabilize the open conformation of LFA-1. Our data suggest that Ndr2 activation is a crucial step to initiate TCR-mediated LFA-1 activation in T cells.

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  1. [1]Hogg N Patzak I Willenbrock F. The insider's guide to leukocyte integrin signalling and function. Nat Rev Immunol. (2011) 11: 416– 26. 10.1038/nri2986 21597477 · DOI
  2. [2]Kinashi T. Intracellular signalling controlling integrin activation in lymphocytes. Nat Rev Immunol. (2005) 5: 546– 59. 10.1038/nri1646 15965491 · DOI
  3. [3]Stewart MP McDowall A Hogg N. LFA-1-mediated adhesion is regulated by cytoskeletal restraint and by a Ca2+-dependent protease, calpain. J Cell Biol. (1998) 140: 699– 707. 10.1083/jcb.140.3.699 9456328 PMC2140165 · DOI
  4. [4]Abram CL Lowell CA. Leukocyte adhesion deficiency syndrome: a controversy solved. Immunol Cell Biol. (2009) 87: 440– 2. 10.1038/icb.2009.32 19417769 PMC4464832 · DOI
  5. [5]Takala H Nurminen E Nurmi SM Aatonen M Strandin T Takatalo M. β2 integrin phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding. Blood (2008) 112: 1853– 62. 10.1182/blood-2007-12-127795 18550856 · DOI
  6. [6]Ishihara S Nishikimi A Umemoto E Miyasaka M Saegusa M Katagiri K. Dual functions of Rap1 are crucial for T-cell homeostasis and prevention of spontaneous colitis. Nat Commun. (2015) 6: 8982. 10.1038/ncomms9982 26634692 PMC4686857 · DOI
  7. [7]Li YF Tang RH Puan KJ Law SK Tan SM. The cytosolic protein talin induces an intermediate affinity integrin alphaLbeta2. J Biol Chem. (2007) 282: 24310– 9. 10.1074/jbc.M701860200 17591777 · DOI
  8. [8]Simonson WT Franco SJ Huttenlocher A. Talin1 regulates TCR-mediated LFA-1 function. J Immunol. (2006) 177: 7707– 14. 10.4049/jimmunol.177.11.7707 17114441 · DOI
  9. [9]Wernimont SA Wiemer AJ Bennin DA Monkley SJ Ludwig T Critchley DR. Contact-dependent T cell activation and T cell stopping require talin1. J Immunol. (2011) 187: 6256– 67. 10.4049/jimmunol.1102028 22075696 PMC3237745 · DOI
  10. [10]Kondo N Ueda Y Kita T Ozawa M Tomiyama T Yasuda K. NDR1-dependent regulation of kindlin-3 controls high-affinity LFA-1 binding and immune synapse organization. Mol Cell Biol. (2017) 37: e00424– 16. 10.1128/MCB.00424-16 28137909 PMC5376635 · DOI
  11. [11]Manevich-Mendelson E Feigelson SW Pasvolsky R Aker M Grabovsky V Shulman Z Kilic Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood (2009) 114: 2344– 53. 10.1182/blood-2009-04-218636 19617577 · DOI
  12. [12]Margadant C Monsuur HN Norman JC Sonnenberg A Mechanisms of integrin activation and trafficking Curr Opin Cell Biol. (2011) 23: 607– 14. 10.1016/j.ceb.2011.08.005 21924601 · DOI
  13. [13]Gahmberg CG Fagerholm SC Nurmi SM Chavakis T Marchesan S Gronholm M. Regulation of integrin activity and signalling. Biochim Biophys Acta (2009) 1790: 431– 44. 10.1016/j.bbagen.2009.03.007 19289150 PMC2734279 · DOI
  14. [14]Kiema T Lad Y Jiang P Oxley CL Baldassarre M Wegener KL. The molecular basis of filamin binding to integrins and competition with talin. Mol Cell (2006) 21: 337– 47. 10.1016/j.molcel.2006.01.011 16455489 · DOI · Paper page
  15. [15]Bos JL de Rooij J Reedquist KA Rap1 signaling: adhering to new models. Nat Rev Mol Cell Biol. (2001) 2: 369– 77. 10.1038/35073073 11331911 · DOI
  16. [16]Katagiri K Maeda A Shimonaka M Kinashi T. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat Immunol. (2003) 4: 741– 8. 10.1038/ni950 12845325 · DOI
  17. [17]Lafuente EM van Puijenbroek AA Krause M Carman CV Freeman GJ Berezovskaya A. RIAM, an Ena/VASP and profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev Cell (2004) 7: 585– 95. 10.1016/j.devcel.2004.07.021 15469846 · DOI
  18. [18]Patsoukis N Bardhan K Weaver JD Sari D Torres-Gomez A Li L. The adaptor molecule RIAM integrates signaling events critical for integrin-mediated control of immune function and cancer progression. Sci Signal (2017) 10: eaam8298. 10.1126/scisignal.aam8298 28831022 · DOI
  19. [19]Witte A Degen J Baumgart K Waldt N Kuropka B Freund C Emerging roles of ADAP, SKAP55, and SKAP-HOM for integrin and NF-κB signaling in T cells. Clin Cell Immunol. (2012) S 12: 002 1– 8. 10.4172/2155-9899.S12-002 · DOI
  20. [20]Wang H Rudd CE. SKAP-55, SKAP-55-related and ADAP adaptors modulate integrin-mediated immune-cell adhesion. Trends Cell Biol. (2008) 18: 486– 93. 10.1016/j.tcb.2008.07.005 18760924 PMC3512129 · DOI
  21. [21]Griffiths EK Krawczyk C Kong YY Raab M Hyduk SJ Bouchard D. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science (2001) 293: 2260– 3. 10.1126/science.1063397 11567140 · DOI
  22. [22]Peterson EJ Woods ML Dmowski SA Derimanov G Jordan MS Wu JN. Coupling of the TCR to integrin activation by Slap-130/Fyb. Science (2001) 293: 2263– 5. 10.1126/science.1063486 11567141 · DOI
  23. [23]Wang H Moon EY Azouz A Wu X Smith A Schneider H. SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat Immunol. (2003) 4: 366– 74. 10.1038/ni913 12652296 · DOI
  24. [24]Menasche G Kliche S Chen EJ Stradal TE Schraven B Koretzky G. RIAM links the ADAP/SKAP-55 signaling module to Rap1, facilitating T-cell-receptor-mediated integrin activation. Mol Cell Biol. (2007) 27: 4070– 81. 10.1128/MCB.02011-06 17403904 PMC1900018 · DOI
  25. [25]Raab M Wang H Lu Y Smith X Wu Z Strebhardt K. T cell receptor “inside-out” pathway via signaling module SKAP1-RapL regulates T cell motility and interactions in lymph nodes. Immunity (2010) 32: 541– 56. 10.1016/j.immuni.2010.03.007 20346707 PMC3812847 · DOI
  26. [26]Lim D Lu Y Rudd CE. Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1. Immunol Lett. (2016) 172: 40– 46. 10.1016/j.imlet.2016.02.004 26905930 PMC4860717 · DOI
  27. [27]Ophir MJ Liu BC Bunnell SC. The N terminus of SKAP55 enables T cell adhesion to TCR and integrin ligands via distinct mechanisms. J Cell Biol. (2013) 203: 1021– 41. 10.1083/jcb.201305088 24368808 PMC3871428 · DOI
  28. [28]Raab M Smith X Matthess Y Strebhardt K Rudd CE. SKAP1 protein PH domain determines RapL membrane localization and Rap1 protein complex formation for T cell receptor (TCR) activation of LFA-1. J Biol Chem. (2011) 286: 29663– 70. 10.1074/jbc.M111.222661 21669874 PMC3191007 · DOI
  29. [29]Raab M Lu Y Kohler K Smith X Strebhardt K Rudd CE. LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation. Nat Commun. (2017) 8: 16001. 10.1038/ncomms16001 28699640 PMC5510181 · DOI
  30. [30]Avruch J Zhou D Fitamant J Bardeesy N Mou F Barrufet LR. Protein kinases of the Hippo pathway: regulation and substrates. Semin Cell Dev Biol. (2012) 23: 770– 84. 10.1016/j.semcdb.2012.07.002 22898666 PMC3489012 · DOI
  31. [31]Katagiri K Imamura M Kinashi T. Spatiotemporal regulation of the kinase Mst1 by binding protein RAPL is critical for lymphocyte polarity and adhesion. Nat Immunol. (2006) 7: 919– 28. 10.1038/ni1374 16892067 · DOI
  32. [32]Katagiri K Katakai T Ebisuno Y Ueda Y Okada T Kinashi T. Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes. EMBO J. (2009) 28: 1319– 31. 10.1038/emboj.2009.82 19339990 PMC2683056 · DOI
  33. [33]Zhou D Medoff BD Chen L Li L Zhang XF Praskova M. The Nore1B/Mst1 complex restrains antigen receptor-induced proliferation of naive T cells. Proc Natl Acad Sci USA (2008) 105: 20321– 6. 10.1073/pnas.0810773105 19073936 PMC2600581 · DOI
  34. [34]Hergovich A Stegert MR Schmitz D Hemmings BA. NDR kinases regulate essential cell processes from yeast to humans. Nat Rev Mol Cell Biol. (2006) 7: 253– 64. 10.1038/nrm1891 16607288 · DOI
  35. [35]Hergovich A. The roles of NDR protein kinases in hippo signalling. Genes (2016) 7: 1– 16. 10.3390/genes7050021 27213455 PMC4880841 · DOI
  36. [36]Tang F Gill J Ficht X Barthlott T Cornils H Schmitz-Rohmer D. The kinases NDR1/2 act downstream of the Hippo homolog MST1 to mediate both egress of thymocytes from the thymus and lymphocyte motility. Sci Signal (2015) 8: ra100. 10.1126/scisignal.aab2425 26443704 · DOI
  37. [37]Schmitz-Rohmer D Probst S Yang ZZ Laurent F Stadler MB Zuniga A. NDR kinases are essential for somitogenesis and cardiac looping during mouse embryonic development. PLoS ONE (2015) 10: e0136566. 10.1371/journal.pone.0136566 26305214 PMC4549247 · DOI
  38. [38]Cornils H Stegert MR Hergovich A Hynx D Schmitz D Dirnhofer S. Ablation of the kinase NDR1 predisposes mice to the development of T cell lymphoma. Sci Signal (2010) 3: ra47. 10.1126/scisignal.2000681 20551432 · DOI · Paper page
  39. [39]Demiray YE Rehberg K Kliche S Stork O. Ndr2 kinase controls neurite outgrowth and dendritic branching through α1 integrin expression. Front Mol Neurosci. (2018) 11: 66. 10.3389/fnmol.2018.00066 29559888 PMC5845635 · DOI · Paper page
  40. [40]Rehberg K Kliche S Madencioglu DA Thiere M Müller B Meineke BM. The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth. J Neurosci. (2014) 34: 5342– 54. 10.1523/JNEUROSCI.2728-13.2014 24719112 PMC6609001 · DOI · Paper page
  41. [41]Kliche S Breitling D Togni M Pusch R Heuer K Wang X. The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1. Mol Cell Biol. (2006) 26: 7130– 44. 10.1128/MCB.00331-06 16980616 PMC1592884 · DOI
  42. [42]Devroe E Erdjument-Bromage H Tempst P Silver PA. Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. (2004) 279: 24444– 5 10.1074/jbc.M401999200 15067004 · DOI
  43. [43]Muriel O Echarri A Hellriegel C Pavón DM Beccari L Del Pozo MA. Phosphorylated filamin A regulates actin-linked caveolae dynamics. J Cell Sci. (2011) 124: 2763– 76. 10.1242/jcs.080804 21807941 · DOI
  44. [44]Travis MA van der Flier A Kammerer RA Mould AP Sonnenberg A Humphries MJ. Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation. FEBS Lett. (2004) 569: 185– 90. 10.1016/j.febslet.2004.04.099 15225631 · DOI
  45. [45]Moser M Bauer M Schmid S Ruppert R Schmidt S Sixt M. Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells. Nat Med. (2009) 15: 300– 5. 10.1038/nm.1921 19234461 · DOI
  46. [47]Witte A Meineke B Sticht J Philipsen L Kuropka B Müller AJ. D120 and K152 within the PH Domain of T cell adapter SKAP55 regulate plasma membrane targeting of SKAP55 and LFA-1 affinity modulation in human T lymphocytes. Mol Cell Biol. (2017) 37: e00509– 16. 10.1128/MCB.00509-16 28052935 PMC5359422 · DOI
  47. [48]Parzmair GP Gereke M Haberkorn O Annemann M Podlasly L Kliche S. ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens. J Leukoc Biol. (2017) 101: 407– 19. 10.1189/jlb.1A0216-090RR 27605210 · DOI
  48. [49]Horn J Wang X Reichardt P Stradal TE Warnecke N Simeoni L. Src homology 2-domain containing leukocyte-specific phosphoprotein of 76 kDa is mandatory for TCR-mediated inside-out signaling, but dispensable for CXCR4-mediated LFA-1 activation, adhesion, and migration of T cells. J Immunol. (2009) 183: 5756– 67. 10.4049/jimmunol.0900649 19812192 · DOI
  49. [50]Wang X Simeoni L Lindquist JA Saez-Rodriguez J Ambach A Gilles ED. Dynamics of proximal signaling events after TCR/CD8-mediated induction of proliferation or apoptosis in mature CD8+ T cells. J Immunol. (2008) 180: 6703– 12. 10.4049/jimmunol.180.10.6703 18453590 · DOI
  50. [51]Kliche S Worbs T Wang X Degen J Patzak I Meineke B. CCR7-mediated LFA-1 functions in T cells are regulated by 2 independent ADAP/SKAP55 modules. Blood (2012) 119: 777– 85. 10.1182/blood-2011-06-362269 22117043 · DOI
  51. [52]Hutti JE Jarrell ET Chang JD Abbott DW Storz P Toker A. A rapid method for determining protein kinase phosphorylation specificity. Nat Methods (2004) 1: 27– 9. 10.1038/nmeth708 15782149 · DOI
  52. [53]Sato T Ishii J Ota Y Sasaki E Shibagaki Y Hattori S. Mammalian target of rapamycin (mTOR) complex 2 regulates filamin A-dependent focal adhesion dynamics and cell migration. Genes Cells (2016) 21: 579– 93. 10.1111/gtc.12366 27059097 · DOI
  53. [54]Suzuki JI Yamasaki S Wu J Koretzky GA Saito T. The actin cloud induced by LFA-1–mediated outside-in signals lowers the threshold for T-cell activation. Blood (2007) 109: 168– 75. 10.1182/blood-2005-12-020164 16973965 · DOI
  54. [55]Perez OD Mitchell D Jager GC South S Murriel C McBride J. Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1. Nat Immunol. (2003) 4: 1083– 92. 10.1038/ni984 14528303 · DOI
  55. [56]Stegert MR Hergovich A Tamaskovic R Bichsel SJ Hemmings B. Regulation of NDR protein kinase by hydrophobic motif phosphorylation mediated by the mammalian Ste20-like kinase MST3. Mol Cell Biol. (2005). 25: 11019– 29. 10.1128/MCB.25.24.11019-11029.2005 16314523 PMC1316964 · DOI · Paper page
  56. [57]Ravid D Chuderland D Landsman L Lavie Y Reich R Liscovitch M. Filamin A is a novel caveolin-1-dependent target in IGF-I-stimulated cancer cell migration. Exp Cell Res. (2008) 314: 2762– 73. 10.1016/j.yexcr.2008.06.004 18598695 · DOI
  57. [58]Woo MS Ohta Y Rabinovitz I Stossel TP Blenis J. Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site. Mol Cell Biol. (2004) 24: 3025– 35. 10.1128/MCB.24.7.3025-3035.2004 15024089 PMC371131 · DOI
  58. [59]Li L Lu L Chen F Filamin A phosphorylation by Akt promotes cell migration in response to arsenic. Oncotarget (2015) 20: 12009– 19. 10.18632/oncotarget.3617 PMC4494919 25944616 · DOI
  59. [60]Davies P Shizuta Y Olden K Gallo M Pastan I. Phosphorylation of filamin and other proteins in cultured fibroblasts. Biochem Biophys Res Commun. (1977) 74: 300– 7. 10.1016/0006-291X(77)91408-5 189763 · DOI
  60. [61]Wallach D Davies PJ Pastan I. Cyclic AMP-dependent phosphorylation of filamin in mammalian smooth muscle. J Biol Chem. (1978) 253: 4739– 45. 207708
  61. [62]Carroll RC Gerrard JM. Phosphorylation of platelet actin-binding protein during platelet activation. Blood (1982) 59: 466– 71. 7199358
  62. [63]Chen M Stracher A. In situ phosphorylation of platelet actin-binding protein by cAMP-dependent protein kinase stabilizes it against proteolysis by calpain. J Biol Chem (1989) 264: 14282– 9. 2547793
  63. [64]Wu MP Jay D Stracher A. Existence of multiple phosphorylated forms of human platelet actin binding protein. Cell Mol Biol Res. (1994) 40: 351– 7. 7866435
  64. [65]Pons M Izquierdo I Andreu-Carbo M Garrido G Planagumà J Muriel O. Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling. J Cell Sci (2017) 130: 490– 501. 10.1242/jcs.193821 27909248 · DOI
  65. [66]Feigelson SW Grabovsky V Manevich-Mendelson E Pasvolsky R Shulman Z. Kindlin-3 is required for the stabilization of TCR-stimulated LFA-1:ICAM-1 bonds critical for lymphocyte arrest and spreading on dendritic cells. Blood (2011) 117: 7042– 52. 10.1182/blood-2010-12-322859 21536861 · DOI
  66. [67]Morrison VL Uotila LM Llort Asens M Savinko T Fagerholm SC. Optimal T cell activation and B cell antibody responses in vivo require the interaction between leukocyte function-associated antigen-1 and kindlin-3. J Immunol. (2015) 195: 105– 15. 10.4049/jimmunol.1402741 25987740 · DOI
  67. [68]Vadlamudi RK Li F Adam L Nguyen D Ohta Y Stossel TP. Filamin is essential in actin cytoskeletal assembly mediated by p21-activated kinase 1. Nat Cell Biol. (2002) 4: 681– 90. 10.1038/ncb838 12198493 · DOI
  68. [69]Phee H Abraham RT Weiss A. Dynamic recruitment of PAK1 to the immunological synapse is mediated by PIX independently of SLP-76 and Vav1. Nat Immunol. (2005) 6: 608– 17. 10.1038/ni1199 15864311 · DOI
  69. [70]Missy K Hu B Schilling K Harenberg A Sakk V Kuchenbecker K. AlphaPIX Rho GTPase guanine nucleotide exchange factor regulates lymphocyte functions and antigen receptor signaling. Mol Cell Biol. (2008) 28: 3776– 89. 10.1128/MCB.00507-07 18378701 PMC2423308 · DOI
  70. [71]Ku GM Yablonski D Manser E Lim L Weiss A. A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT. EMBO J. (2001) 20: 457– 65. 10.1093/emboj/20.3.457 11157752 PMC133476 · DOI
  71. [72]Kim MJ Kim E Ryu SH Suh PG. The mechanism of phospholipase C-gamma1 regulation. Exp Mol Med. (2000) 32: 101– 9. 10.1038/emm.2000.18 11048639 · DOI
  72. [73]Katagiri K Shimonaka M Kinashi T. Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1. J Biol Chem. (2004) 279: 11875– 81. 10.1074/jbc.M310717200 14702343 · DOI

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Authors
Waldt, N., Seifert, A., Demiray, Y. E., Devroe, E., Turk, B. E., Reichardt, P., Mix, C., Reinhold, A., Freund, C., Müller, A. J., Schraven, B., Stork, O., & Kliche, S.
Affiliations
  • Otto-von-Guericke-Universität Magdeburg (DE)
  • The University of Texas MD Anderson Cancer Center (US)
  • Yale University (US)
  • Freie Universität Berlin (DE)
  • Helmholtz Centre for Infection Research (DE)
  • Center for Behavioral Brain Sciences (DE)
Corresponding author
  • Schraven, Burkhart
  • Stork, Oliver
  • Kliche, Stefanie
ORCID
Venue
Frontiers in Immunology
Publisher
Frontiers Media SA
Published
2018-12-04
Volume
9
Pages
2852-2852
ISSN
1664-3224 (ISSN-L)
DOI
10.3389/fimmu.2018.02852
PMC
PMC6290345
OpenAlex
W2902466408
Cited by
31
References
1
Citations by year
2026: 4 · 2025: 7 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 2
FWCI
2.88
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Immunology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • HZI (Helmholtz Centre for Infection Research) (submittedVersion, cc-by-nc-sa)
  • Refubium (Universitätsbibliothek der Freien Universität Berlin) (submittedVersion, cc-by)
  • Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver (cc-by)
Funders
Deutsche Forschungsgemeinschaft (CRC854/B10, and Z01, CRC854)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
filamin A, LFA-1, integrin activation, T cell receptor signaling, serine phosphorylation, talin, kindlin-3, integrin conformation, T cell-APC interaction
MeSH terms
Animals, Cells, Cultured, Cytoskeletal Proteins, Humans, Lymphocyte Activation, Membrane Proteins, Mutation, Neoplasm Proteins, Phosphorylation, Proteins, Receptors, Antigen, T-Cell, Serine, T-Lymphocytes, Lymphocyte Function-Associated Antigen-1, Talin, Protein Serine-Threonine Kinases, Mice, Knockout, CD18 Antigens, Jurkat Cells, Adaptor Proteins, Signal Transducing, Mice, HEK293 Cells, Primary Cell Culture, Healthy Volunteers, Filamins

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