Production of ergothioneine by Methylobacterium species
Open accessAbstract
Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize EGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 μg/100 ml culture (1200 μg/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.
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ERG is synthesised by a variety of microbes, especially fungi and actinobacteria.
“The chief organisms capable of its synthesis are fungi and certain yeasts(59,60), though actinobacteria and certain other micro-organisms(60–66), including the slime mould Physarum polycephalum (65), cyanobacteria(67–71) and methylotrophs(72) are also naturally capable of its production.”
From The biology of ergothioneine, an antioxidant nutraceutical (2020) · cites this paper as [72]
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Fahey, R. C. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.333 · Cited by 266 · see citing works
The evolutionary history of the genes involved in the biosynthesis of the antioxidant ergothioneine
Jones, G. W., Doyle, S., & Fitzpatrick, D. A. (2014) · Gene · doi:10.1016/j.gene.2014.07.065 · Cited by 79 · see citing works
Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses
Nakajima, S., Satoh, Y., Yanashima, K., Matsui, T., & Dairi, T. (2015) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2015.01.013 · Cited by 38 · see citing works
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The biology of ergothioneine, an antioxidant nutraceutical
Borodina, I., Kenny, L. C., McCarthy, C. M., Paramasivan, K., Pretorius, E., Roberts, T. J., van der Hoek, S. A., & Kell, D. B. (2020) · Nutrition Research Reviews · doi:10.1017/s0954422419000301 · Cited by 296 · see citing works
“though actinobacteria and certain other micro-organisms(60–66), including the slime mould Physarum polycephalum(65), cyanobacteria(67–71) and methylotrophs(72) are also naturally”
Background
- Authors
- Alamgir, K. M., Masuda, S., Fujitani, Y., Fukuda, F., & Tani, A.
- Affiliations
- Okayama University (JP)
- Corresponding author
- Tani, Akio
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2015-10-27
- Volume
- 6
- Pages
- 1185-1185
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC4621440
- OpenAlex
- W2172584831
- Cited by
- 100
- References
- 3
- Citations by year
- 2026: 14 · 2025: 21 · 2024: 16 · 2023: 5 · 2022: 13 · 2021: 8 · 2020: 7 · 2019: 4 · 2018: 7 · 2017: 4 · 2016: 1
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Japan Science and Technology Agency (#AS251Z00826N)
- Noda Institute for Scientific Research
- Advanced Low Carbon Technology Research and Development Program (ALCA)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- ergothioneine, Methylobacterium, ergothioneine biosynthesis, methylotrophy, phyllosphere, methanol fermentation, antioxidant amino acids, deletion mutants
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