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Cited by 25Wastewater Treatment and Nitrogen RemovalMicrobial Community Ecology and PhysiologySoil Carbon and Nitrogen Dynamics

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Physiological and Metagenomic Characterizations of the Synergistic Relationships between Ammonia- and Nitrite-Oxidizing Bacteria in Freshwater Nitrification

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Cai, Mingwei, Ng, Siu-Kin, Lim, Chee Kent, Lu, Hongyuan, Jia, Yangyang, Lee, Patrick K. H.·2018·Frontiers in Microbiology
DOI 10.3389/fmicb.2018.00280PMC PMC5835065
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Abstract

Nitrification plays a crucial role in global nitrogen cycling and treatment processes. However, the relationships between the nitrifier guilds of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) are still poorly understood, especially in freshwater habitats. This study examined the physiological interactions between the AOB and NOB present in a freshwater aquarium biofilter by culturing them, either together or separately, in a synthetic medium. Metagenomic and 16S rRNA gene sequencing revealed the presence and the draft genomes of Nitrosomonas-like AOB as well as Nitrobacter-like NOB in the cultures, including the first draft genome of Nitrobacter vulgaris. The nitrifiers exhibited different growth rates with different ammonium (NH4+) or nitrite concentrations (50 to 1,500 μM) and the growth rates were elevated under a high bicarbonate (HCO3-) concentration. The half-saturation constant (Ks for NH4+), the maximum growth rate (μmax), and the lag duration indicated a strong dependence on the synergistic relationships between the two guilds. Overall, the ecophysiological and metagenomic results in this study provided insights into the phylogeny of the key nitrifying players in a freshwater biofilter and showed that interactions between the two nitrifying guilds in a microbial community enhanced nitrification.

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Metadata

Authors
Cai, M., Ng, S.-K., Lim, C. K., Lu, H., Jia, Y., & Lee, P. K. H.
Affiliations
City University of Hong Kong (HK)
Corresponding author
Lee, Patrick K. H.
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2018-02-27
Volume
9
Pages
280-280
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2018.00280
PMC
PMC5835065
OpenAlex
W2793300115
Cited by
25
References
84
Citations by year
2026: 2 · 2025: 5 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 1
FWCI
0.82
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • CityU Scholars (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Research Grants Council, University Grants Committee (116413)
Topics
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
Keywords
nitrification, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, Nitrosomonas, Nitrobacter, nitrogen cycling

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